The purpose of this research is to investigate the effect of land use, built environment and public transportation facilities’ locations on destinations of bike-sharing trips in an urban setting. Several methods have been applied to determine the relationship between predicting variables and trip destinations, such as ordinary least squares regression, spatial regression and geographically weighted regression. Additionally, a comparison between the proposed models, count models and random forest has been conducted. The data were collected in Budapest, Hungary. It has been found that touristic points of interest, and healthcare and educational points have a positive impact on bike-sharing destinations. Public transportation stops for buses, trains and trams attract bike-sharing users, which has a potential for the bike-and-ride system. Land use has different effects on bike-sharing trip destinations; mostly as a circular shape variation within the urban structure of the city, such as residential, industrial, commercial and educational zones. Other variables, such as road length and water areas, form as constraints to bike-sharing trip destinations. Geographically weighted and spatial regression performs better than count models and random forest. This study helps decision-makers in predicting the origin-destination matrix of bike-sharing trips based on the transportation network and land use.