Cluster of ribosomal protein genes in Escherichia coli containing genes for proteins S6, S18, and L9.

Isono, Katsumi; Kitakawa, Madoka

doi:10.1073/pnas.75.12.6163

Cited by 26 publications

(9 citation statements)

References 19 publications

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“…2;Russell, 1972;Isono & Kitakawa, 1978); our data are consistently in agreement with the higher value. Cross 4 shows the expected low degree of cotransduction to valS.…”

Section: Results a N D Discussionsupporting

confidence: 94%

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Isolation of Escherichia coli Mutants (cpdB) Deficient in Periplasmic 2' :3' -Cyclic Phosphodiesterase and Genetic Mapping of the cpdB Locus

Beacham¹,

Garrett²

1980

Microbiology

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“…2;Russell, 1972;Isono & Kitakawa, 1978); our data are consistently in agreement with the higher value. Cross 4 shows the expected low degree of cotransduction to valS.…”

Section: Results a N D Discussionsupporting

confidence: 94%

“…Values are the percentage cotransduction; the arrows point to the selected marker. Values below the map are from: a, Russell (1972), and 6,Isono & Kitakawa (1978).…”

mentioning

confidence: 99%

Isolation of Escherichia coli Mutants (cpdB) Deficient in Periplasmic 2' :3' -Cyclic Phosphodiesterase and Genetic Mapping of the cpdB Locus

Beacham¹,

Garrett²

1980

Microbiology

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“…RsgA, RbfA and RimM play their roles in maturation of the 30S subunit at different timings: first RimM, then RbfA, and last RsgA [8], [20]. We also focused on the effects of deletion of rpsF encoding the ribosomal protein S6 [21], which is available from the Keio collection, and rrmJ , a gene for methyltransferase (RrmJ) that catalyzes the 2′- O -methylation of the ribose at U2552 of 23S rRNA [22].…”

Section: Resultsmentioning

confidence: 99%

Impairment of Ribosome Maturation or Function Confers Salt Resistance on Escherichia coli Cells

et al. 2013

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We found that loss of integrity of the ribosome by removal of a putative ribosome maturation factor or a ribosomal protein conferred salt tolerance on Escherichia coli cells. Some protein synthesis inhibitors including kasugamycin and chloramphenicol also had a similar effect, although kasugamycin affected neither 16S rRNA maturation nor subunit association into a 70S ribosome. Thus, salt tolerance is a common feature of cells in which maturation or function of the ribosome is impaired. In these cells, premature induction of an alternative sigma factor, σE, by salt stress was observed. These results suggest the existence of a yet-unknown stress response pathway mediated by the bacterial ribosome.

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“…However, in β-proteobactera and the remaining γ-proteobacteria they are separated by priB, and in Firmicutes and Actinobacteria they are separated by ssbA. In E. coli the operon contains rplI (encoding L9) (Isono and Kitakawa 1978), which occurs in isolation at a different …”

Section: Resultsmentioning

confidence: 99%

Bacterial RNA motif in the 5′ UTR of rpsF interacts with an S6:S18 complex

Deiorio-Haggar

Soo

et al. 2013

RNA

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Approximately half the transcripts encoding ribosomal proteins in Escherichia coli include a structured RNA motif that interacts with a specific ribosomal protein to inhibit gene expression, thus allowing stoichiometric production of ribosome components. However, many of these RNA structures are not widely distributed across bacterial phyla. It is increasingly common for RNA motifs associated with ribosomal protein genes to be identified using comparative genomic methods, yet these are rarely experimentally validated. In this work, we characterize one such motif that precedes operons containing rpsF and rpsR, which encode ribosomal proteins S6 and S18. This RNA structure is widely distributed across many phyla of bacteria despite differences within the downstream operon, and examples are present in both E. coli and Bacillus subtilis. We demonstrate a direct interaction between an example of the RNA from B. subtilis and an S6:S18 complex using in vitro binding assays, verify our predicted secondary structure, and identify a putative protein-binding site. The proposed binding site bears a strong resemblance to the S18 binding site within the 16S rRNA, suggesting molecular mimicry. This interaction is a valuable addition to the canon of ribosomal protein mRNA interactions. This work shows how experimental verification translates computational results into concrete knowledge of biological systems.

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Cluster of ribosomal protein genes in Escherichia coli containing genes for proteins S6, S18, and L9.

Cited by 26 publications

References 19 publications

Isolation of Escherichia coli Mutants (cpdB) Deficient in Periplasmic 2' :3' -Cyclic Phosphodiesterase and Genetic Mapping of the cpdB Locus

Isolation of Escherichia coli Mutants (cpdB) Deficient in Periplasmic 2' :3' -Cyclic Phosphodiesterase and Genetic Mapping of the cpdB Locus

Impairment of Ribosome Maturation or Function Confers Salt Resistance on Escherichia coli Cells

Bacterial RNA motif in the 5′ UTR of rpsF interacts with an S6:S18 complex

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