Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Genetic Engineering: Robotic Genetic Surgery

Deshpande, Kaivalya; Vyas, Arpita; Balakrishnan, Archana; Vyas, Dinesh

doi:10.1166/ajrs.2015.1023

Cited by 20 publications

(10 citation statements)

References 29 publications

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“… 5 The clustered regularly interspersed short palindromic repeats (CRISPR)/Cas system, especially CRISPR-associated protein 9 (CRISPR/Cas9), is a widely used prokaryotic nuclease-based target gene precise editing tool, which known as an effective alternative to ZFNs and TALENs. 5 , 141 , 142 CRISPR/Cas9, identified as the most popular genetic engineering technique, causes genome modifications by delivering to plant cells and expressing there. 129 , 142 , 143 In this method, the single guide RNA (sgRNA) directs the caspase to the target DNA sites and helps breeding low immunogenic epitopes containing plants with greater specificity.…”

Section: Methodsmentioning

confidence: 99%

The Gluten Gene: Unlocking the Understanding of Gluten Sensitivity and Intolerance

Asri¹,

Rostami-Nejad²,

Rostami³

2021

TACG

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Wheat flour is one of the most important food ingredients containing several essential nutrients including proteins. Gluten is one of the major protein components of wheat consisted of glutenin (encoded on chromosome 1) and gliadin (encoded on chromosome 1 and 6) and there are around hundred genes encoding it in wheat. Gluten proteins have the ability of eliciting the pathogenic immune responses and hypersensitivity reactions in susceptible individuals called “gluten-related disorders (GRDs)”, which include celiac disease (CD), wheat allergy (WA), and non-celiac gluten sensitivity (NCGS). Currently removing gluten from the diet is the only effective treatment for mentioned GRDs and studies for the appropriate and alternative therapeutic approaches are ongoing. Accordingly, several genetic studies have focused on breeding wheat with low immunological properties through gene editing methods. The present review considers genetic characteristics of gluten protein components, focusing on their role in the incidence of gluten-related diseases, and genetic modifications conducted to produce wheat with less immunological properties.

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Section: Methodsmentioning

confidence: 99%

The Gluten Gene: Unlocking the Understanding of Gluten Sensitivity and Intolerance

Asri¹,

Rostami-Nejad²,

Rostami³

2021

TACG

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“…The various RNA seq-based approaches to therapy of various diseases include gene therapy[41], natural antisense transcripts (NATs), antisense oligonucleotides (ASOs), and plasmid based therapy (Figure 2). …”

Section: Rna Sequencing For Treatment Of Colorectal Diseasesmentioning

confidence: 99%

“…Through specific targeting, RNA sequencing allows for the development of new therapeutic approaches to colorectal diseases. The various RNA seq-based approaches to therapy of various diseases include gene therapy[ 41 ], natural antisense transcripts (NATs), antisense oligonucleotides (ASOs), and plasmid based therapy (Figure 2 ).…”

Section: Rna Sequencing For Treatment Of Colorectal Diseasesmentioning

confidence: 99%

High throughput RNA sequencing utility for diagnosis and prognosis in colon diseases

Gao

Zhong

Patel

et al. 2017

WJG

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RNA sequencing is the use of high throughput next generation sequencing technology to survey, characterize, and quantify the transcriptome of a genome. RNA sequencing has been used to analyze the pathogenesis of several malignancies such melanoma, lung cancer, and colorectal cancer. RNA sequencing can identify differential expression of genes (DEG’s), mutated genes, fusion genes, and gene isoforms in disease states. RNA sequencing has been used in the investigation of several colorectal diseases such as colorectal cancer, inflammatory bowel disease (ulcerative colitis and Crohn’s disease), and irritable bowel syndrome.

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“…Prior to the discovery of CRISPR/Cas9 enzyme, RNA sequencing[7,8], modular protein recognition DNA such as zinc finger nuclease or TAL effector nuclease were used in epigenetic modification and disease modeling, which are complex and require very specific protein engineering and programming[13]. However, with the revolutionary CRISPR technology, researchers now may edit genomes in a much simpler and precise way as modeled by bacteria[14,15].…”

Section: Introductionmentioning

confidence: 99%

“…CRISPR/Cas9 was found in Streptococcus pyogenes, which uses this mechanism as defense against invading viruses[14]. Upon infection by a virus the bacteria stores the viral DNA sequence in between sections of regularly interspaced palindromic repeated segments that are closely associated with genes that encode CRISPR-associated proteins.…”

Section: Introductionmentioning

confidence: 99%

Harnessing the potential of gene editing technology using CRISPR in inflammatory bowel disease

Limanskiy

Vyas

Chaturvedi

et al. 2019

WJG

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The molecular scalpel of clustered regularly interspersed short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) technology may be sharp enough to begin cutting the genes implicated in inflammatory bowel disease (IBD) and consequently decrease the 6.3 billion dollar annual financial healthcare burden in the treatment of IBD. For the past few years CRISPR technology has drastically revolutionized DNA engineering and biomedical research field. We are beginning to see its application in gene manipulation of sickle cell disease, human immunodeficiency virus resistant embryologic twin gene modification and IBD genes such as Gatm (Glycine amidinotransferase, mitochondrial), nucleotide-binding oligomerization domain-containing protein 2, KRT12 and other genes implicated in adaptive immune convergence pathways have been subjected to gene editing, however there are very few publications. Furthermore, since Crohn’s disease and ulcerative colitis have shared disease susceptibility and share genetic gene profile, it is paramount and is more advantageous to use CRISPR technology to maximize impact. Although, currently CRISPR does have its limitations due to limited number of specific Cas enzymes, off-target activity, protospacer adjacent motifs and crossfire between different target sites. However, these limitations have given researchers further insight on how to augment and manipulate enzymes to enable precise gene excision and limit crossfire between target sites.

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Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Genetic Engineering: Robotic Genetic Surgery

Cited by 20 publications

References 29 publications

The Gluten Gene: Unlocking the Understanding of Gluten Sensitivity and Intolerance

The Gluten Gene: Unlocking the Understanding of Gluten Sensitivity and Intolerance

High throughput RNA sequencing utility for diagnosis and prognosis in colon diseases

Harnessing the potential of gene editing technology using CRISPR in inflammatory bowel disease

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