The Balkan region has an important geostrategic position in passenger and freight transport between Europe and Asia. This paper studies the development of railway transport on twelve different railway transport markets in the Balkan region. The methodology is based on multi-criteria assessment of the level of railway development. The approach presented in this paper could help railway companies to make decisions about railway transport services. The methodology includes three steps. In the first step, the quantitative and qualitative criteria for the evaluation of the social, economic, infrastructural and technological impact of the level of development of railway transport have been defined. In the second step, the weights of criteria have been determined using both objective and subjective approaches by applying the Shannon Entropy