2024
DOI: 10.1101/2024.09.20.614170
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Clustering of rRNA operons inE. coliis disrupted by σH

Khang Ho,
Rasika M. Harshey

Abstract: Chromosomal organization in E. coli as examined by Hi-C methodology indicates that long-range interactions are sparse. Yet, spatial co-localization or clustering of 6/7 ribosomal RNA (rrn) operons distributed over half the 4.6 Mbp genome has been captured by two other methodologies - fluorescence microscopy and Mu transposition. Our current understanding of the mechanism of clustering is limited to mapping essential cis elements. To identify trans elements, we resorted to perturbing the system by chemical and … Show more

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