Clustering subspecies of Aeromonas salmonicida using IS630typing

Studer, Nina Salouâ; Frey, Joachim; Bergh, Philippe Vanden

doi:10.1186/1471-2180-13-36

Cited by 21 publications

(14 citation statements)

References 47 publications

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“…More complex rearrangements involving other ISs are also suspected. IS 630 copies might also play a role in the genetic organization of the injectisome because they are frequently associated with T3SS loci in A. salmonicida (Studer et al ., ) (http://onlinelibrary.wiley.com/doi/10.1111/1751-7915.12091/suppinfo) but also in other pathogenic bacteria using such secretion systems (Haneda et al ., ; Correa et al ., ; Stavrinides et al ., ). The cluster of T3SS genes can also be integrated in the chromosome of some strains of certain species of Aeromonas .…”

Section: Genetic Organization Of the A Salmonicida T3ssmentioning

confidence: 99%

Aeromonas salmonicida subsp. salmonicida in the light of its type‐three secretion system

Bergh

Frey

2013

Microbial Biotechnology

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Aeromonas salmonicida subsp. salmonicida is an important pathogen in salmonid aquaculture and is responsible for the typical furunculosis. The type-three secretion system (T3SS) is a major virulence system. In this work, we review structure and function of this highly sophisticated nanosyringe in A. salmonicida. Based on the literature as well as personal experimental observations, we document the genetic (re)organization, expression regulation, anatomy, putative functional origin and roles in the infectious process of this T3SS. We propose a model of pathogenesis where A. salmonicida induces a temporary immunosuppression state in fish in order to acquire free access to host tissues. Finally, we highlight putative important therapeutic and vaccine strategies to prevent furunculosis of salmonid fish.

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Section: Genetic Organization Of the A Salmonicida T3ssmentioning

confidence: 99%

Aeromonas salmonicida subsp. salmonicida in the light of its type‐three secretion system

Bergh

Frey

2013

Microbial Biotechnology

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“…Furthermore, many plasmid variants display transpositions or IS-mediated recombinations when compared to their reference (IS AS 5 in many plasmids, IS Ec 9 in pSN254b) (Attéré et al, 2015; Najimi et al, 2009; Trudel et al, 2013; Vincent et al, 2014b). Given the high number of ISs in the genome of this bacterium (Studer, Frey & Vanden Bergh, 2013; Vincent et al, 2016b), we hypothesize that ISs play a role in plasmid reshaping (Tanaka, Frenette & Charette, 2013). …”

Section: Introductionmentioning

confidence: 99%

The mosaic architecture ofAeromonas salmonicidasubsp.salmonicidapAsa4 plasmid and its consequences on antibiotic resistance

Tanaka

Vincent

Trudel

et al. 2016

PeerJ

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Aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in salmonids, is an issue especially because many isolates of this bacterium display antibiotic resistances, which limit treatments against the disease. Recent results suggested the possible existence of alternative forms of pAsa4, a large plasmid found in A. salmonicida subsp. salmonicida and bearing multiple antibiotic resistance genes. The present study reveals the existence of two newly detected pAsa4 variants, pAsa4b and pAsa4c. We present the extensive characterization of the genomic architecture, the mobile genetic elements and the antimicrobial resistance genes of these plasmids in addition to the reference pAsa4 from the strain A449. The analysis showed differences between the three architectures with consequences on the content of resistance genes. The genomic plasticity of the three pAsa4 variants could be partially explained by the action of mobile genetic elements like insertion sequences. Eight additional isolates from Canada and Europe that bore similar antibiotic resistance patterns as pAsa4-bearing strains were genotyped and specific pAsa4 variants could be attributed to phenotypic profiles. pAsa4 and pAsa4c were found in Europe, while pAsa4b was found in Canada. In accordance with their content in conjugative transfer genes, only pAsa4b and pAsa4c can be transferred by conjugation in Escherichia coli. The plasticity of pAsa4 variants related to the acquisition of antibiotic resistance indicates that these plasmids may pose a threat in terms of the dissemination of antimicrobial-resistant A. salmonicida subsp. salmonicida bacteria.

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“…A low quantity of amoebae was able to create a phagocytic plaque on K. aerogenes (the positive control) while only a high quantity of D. discoideum cells was required to observe amoebal growth on JF2480 (Figure 3). Thus, bacterial virulence assays conducted on JF2480 revealed that this strain is virulent when facing D. discoideum even in absence of a complete T3SS compared to K. aerogenes bacterium known to be totally avirulent [47]. Other mesophilic A. salmonicida strains do not possess a complete T3SS.…”

Section: Resultsmentioning

confidence: 92%

A Mesophilic Aeromonas salmonicida Strain Isolated from an Unsuspected Host, the Migratory Bird Pied Avocet

et al. 2019

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Aeromonas salmonicida is a Gram-negative bacterium, known as a fish pathogen since its discovery. Although the species was initially considered psychrophilic, a mesophilic subspecies (pectinolytica) and many other mesophilic strains still not attributed to subspecies have been described in the last two decades. These mesophilic strains were sampled from various sources, including humans, and some of them are known to be pathogenic. In this study, we describe a strain, JF2480, which was isolated from the spleen, and also found the kidney and liver of a dead pied avocet (Recurvirostra avosetta), a type of migratory bird inhabiting aquatic environments. A core genome phylogenomic analysis suggests that JF2480 is taxonomically distant from other known A. salmonicida subspecies. The genome sequence confirms that the strain possesses key virulence genes that are present in the typical A. salmonicida psychrophilic subspecies, with the exception of the genes encoding the type three secretion system (T3SS). Bacterial virulence assays conducted on the surrogate host Dictyostelium discoideum amoeba confirmed that the strain is virulent despite the lack of T3SS. Bacterial growth curves showed that strain JF2480 grow well at 40 °C, the body temperature of the pied avocet, and even faster at 41 °C, compared to other mesophilic strains. Discovery of this strain further demonstrates the extent of the phylogenomic tree of this species. This study also suggests that A. salmonicida can infect a wider array of hosts than previously suspected and that we need to rethink the way we perceive A. salmonicida’s natural environment.

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Clustering subspecies of Aeromonas salmonicida using IS630typing

Cited by 21 publications

References 47 publications

Aeromonas salmonicida subsp. salmonicida in the light of its type‐three secretion system

Aeromonas salmonicida subsp. salmonicida in the light of its type‐three secretion system

The mosaic architecture ofAeromonas salmonicidasubsp.salmonicidapAsa4 plasmid and its consequences on antibiotic resistance

A Mesophilic Aeromonas salmonicida Strain Isolated from an Unsuspected Host, the Migratory Bird Pied Avocet

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