Clusters of circulating Neisseria gonorrhoeae strains and association with antimicrobial resistance in Shanghai

Liao, Mingmin; Bell, K; Gu, Wanyi; Yang, Yang; Eng, Nelson F.; Fu, Wenkai; Wu, Lei; Zhang, Chu-Guang; Chen, Yue; Jolly, Ann; Dillon, Jo-Anne R.

doi:10.1093/jac/dkm544

Cited by 20 publications

(27 citation statements)

References 52 publications

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“…The NG-MAST database, available online (www.ng-mast.net), allows public access for sequence submission and the assignment of sequence types either for porB or tbpB individually or for the assignment of strain types (STs) using a combination of the two loci (11). The objective of the present study was to compare NG-MAST with porB DNA sequence analysis (ϳ82% of the full-length porB gene) to identify circulating clusters of N. gonorrhoeae isolates in Shanghai, China, and to evaluate the correlation between self-reported sexual contacts and genotypes of N. gonorrhoeae isolates.The N. gonorrhoeae isolates (n ϭ 199) were collected from males with gonorrhea (n ϭ 157) and their positive female partners (n ϭ 42), as previously described (8,25). These isolates included 39 pairs and one triplet (one male with two female partners) from patients with self-reported sexual contacts.…”

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confidence: 99%

“…DNA was extracted from the isolates and used for PCR amplification of porB and tbpB. Amplicons were analyzed as previously described (8,11,25). porB DNA sequence analysis covered ϳ82% of the nucleotides encoding surface-exposed loops I to VII and interspace regions II to VII as described and analyzed previously (8,15,22), and porB DNA sequences were deposited in the GenBank database (8).…”

mentioning

confidence: 99%

“…Amplicons were analyzed as previously described (8,11,25). porB DNA sequence analysis covered ϳ82% of the nucleotides encoding surface-exposed loops I to VII and interspace regions II to VII as described and analyzed previously (8,15,22), and porB DNA sequences were deposited in the GenBank database (8). Each isolate was arbitrarily assigned a porB DNA sequence type (PST), and isolates were sorted into groups based on PSTs; groups also defined common clusters of isolates.…”

mentioning

confidence: 99%

“…DNA sequence analysis of various genes is currently the method of choice for distinguishing N. gonorrhoeae strains, as it provides unambiguous and reproducible information and high discriminatory power, and data can be stored or shared electronically, permitting reliable comparisons to be made between laboratories (10,11,23). porB DNA sequence analysis is now commonly used for studying the molecular epidemiology of N. gonorrhoeae (1,9,13,15,16,19,20,23) and involves the sequencing of either the entire gene or various regions of porB (8,(17)(18)(19). The N. gonorrhoeae multiantigen sequence typing (NG-MAST) methodology, which has been applied since 2004 (2,9,13,20,21), is based on limited DNA sequence analyses of two highly polymorphic loci, porB and tbpB (11).…”

mentioning

confidence: 99%

“…The N. gonorrhoeae isolates (n ϭ 199) were collected from males with gonorrhea (n ϭ 157) and their positive female partners (n ϭ 42), as previously described (8,25). These isolates included 39 pairs and one triplet (one male with two female partners) from patients with self-reported sexual contacts.…”

mentioning

confidence: 99%

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Comparison of Neisseria gonorrhoeae Multiantigen Sequence Typing and porB Sequence Analysis for Identification of Clusters of N. gonorrhoeae Isolates

Liao¹,

Helgeson

et al. 2009

J Clin Microbiol

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porB DNA sequence analysis and Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) methods were compared for their abilities to discriminate strains and to identify epidemiologically congruent pairs of N. gonorrhoeae. Both methods provided high-level discrimination of strains. NG-MAST further differentiated large porB-based clusters. However, considerations of cost suggest that porB DNA sequence analysis is a useful tool for preliminary molecular analysis of the epidemiology of N. gonorrhoeae.Molecular typing methods that differentiate Neisseria gonorrhoeae isolates, coupled with traditional epidemiological methods, have been used to identify circulating clusters of strains and transmission networks (3,24). DNA sequence analysis of various genes is currently the method of choice for distinguishing N. gonorrhoeae strains, as it provides unambiguous and reproducible information and high discriminatory power, and data can be stored or shared electronically, permitting reliable comparisons to be made between laboratories (10,11,23). porB DNA sequence analysis is now commonly used for studying the molecular epidemiology of N. gonorrhoeae (1,9,13,15,16,19,20,23) and involves the sequencing of either the entire gene or various regions of porB (8,(17)(18)(19). The N. gonorrhoeae multiantigen sequence typing (NG-MAST) methodology, which has been applied since 2004 (2, 9, 13, 20, 21), is based on limited DNA sequence analyses of two highly polymorphic loci, porB and tbpB (11). The NG-MAST database, available online (www.ng-mast.net), allows public access for sequence submission and the assignment of sequence types either for porB or tbpB individually or for the assignment of strain types (STs) using a combination of the two loci (11). The objective of the present study was to compare NG-MAST with porB DNA sequence analysis (ϳ82% of the full-length porB gene) to identify circulating clusters of N. gonorrhoeae isolates in Shanghai, China, and to evaluate the correlation between self-reported sexual contacts and genotypes of N. gonorrhoeae isolates.The N. gonorrhoeae isolates (n ϭ 199) were collected from males with gonorrhea (n ϭ 157) and their positive female partners (n ϭ 42), as previously described (8,25). These isolates included 39 pairs and one triplet (one male with two female partners) from patients with self-reported sexual contacts. The identification and growth of N. gonorrhoeae isolates were described previously (8, 25). DNA was extracted from the isolates and used for PCR amplification of porB and tbpB. Amplicons were analyzed as previously described (8,11,25). porB DNA sequence analysis covered ϳ82% of the nucleotides encoding surface-exposed loops I to VII and interspace regions II to VII as described and analyzed previously (8,15,22), and porB DNA sequences were deposited in the GenBank database (8). Each isolate was arbitrarily assigned a porB DNA sequence type (PST), and isolates were sorted into groups based on PSTs; groups also defined common clusters of isolates. For NG-MAST analysis, DNA seque...

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

mentioning

confidence: 99%

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Comparison of Neisseria gonorrhoeae Multiantigen Sequence Typing and porB Sequence Analysis for Identification of Clusters of N. gonorrhoeae Isolates

Liao¹,

Helgeson

et al. 2009

J Clin Microbiol

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Antibiograms and randomly amplified polymorphic DNA‐polymerase chain reactions (RAPD‐PCR) as epidemiological markers of gonorrhea

Lawung

Charoenwatanachokchai

Cherdtrakulkiat

et al. 2010

Clinical Laboratory Analysis

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The development of antimicrobial resistance of Neisseria gonorrhoeae arising from wide dissemination of resistant clones is a major global health problem. In this study, a total of 235 isolates of N. gonorrhoeae isolated from patients of Bangrak Hospital were tested for their antibiotic susceptibilities to penicillin, norfloxacin, ofloxacin, ciprofloxacin, spectinomycin, and ceftriaxone. Mutation (Ser-91) in the quinolone resistance determining regions of gyrA and random amplification of the polymorphic DNA polymerase chain reaction (RAPD-PCR) were examined from 145 isolates. Among these, 55 isolates were obtained during January-March 2000, 46 isolates during January-March 2002, and 44 isolates during October-December 2002. The occurrence of combination resistance between penicillin and quinolone was 20% in January-March 2000, which was increased to 57.8% during the period of October-December 2002 (P<0.0001). Mutation of Ser-91 in gyrA could be directly linked with the resistance or declining of susceptibility to ciprofloxacin. Using RAPD-PCR, we could classify the 145 isolates into 4 and 5 groups by primers D11344 (5'-AGTGAATTCGCGGTGAGATGCCA-3') and D8635 (5'-GAGCGGCCAAAGGGAGCA GAC-3'), respectively. Combination of the data obtained from these two primers produced 11 fingerprint groups. Our findings conclude that monitoring of the Ser-91 mutation of gyrA and RAPD-PCR methods are most useful for epidemiological screening.

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Sexual Networks and Sexually Transmitted Infections; “The Strength of Weak (Long Distance) Ties”

Jolly¹,

Wylie²

2012

The New Public Health and STD/HIV Prevention

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Clusters of circulating Neisseria gonorrhoeae strains and association with antimicrobial resistance in Shanghai

Cited by 20 publications

References 52 publications

Comparison of Neisseria gonorrhoeae Multiantigen Sequence Typing and porB Sequence Analysis for Identification of Clusters of N. gonorrhoeae Isolates

Comparison of Neisseria gonorrhoeae Multiantigen Sequence Typing and porB Sequence Analysis for Identification of Clusters of N. gonorrhoeae Isolates

Antibiograms and randomly amplified polymorphic DNA‐polymerase chain reactions (RAPD‐PCR) as epidemiological markers of gonorrhea

Sexual Networks and Sexually Transmitted Infections; “The Strength of Weak (Long Distance) Ties”

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