CMV induces HERV-K and HERV-W expression in kidney transplant recipients

Bergallo, Massimiliano; Galliano, Ilaria; Montanari, Paola; Gambarino, Stefano; Mareschi, Katia; Ferro, Francesca; Fagioli, Franca; Tovo, Pier-Angelo; Ravanini, Paolo

doi:10.1016/j.jcv.2015.04.018

Cited by 51 publications

(36 citation statements)

References 13 publications

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“…Our data suggest that CMV could facilitate HERV activation in vivo. We demonstrated that HERV-K and -W pol gene expression was significantly higher in CMV-infected kidney transplant recipients versus those not infected as previously reported by our groups [17]. Little is currently known about the induction of HERV-K expression, although CpG methylation status of the HERV promoter or their regulatory elements have been suggested to be crucial in the regulation of their activity [18,19].…”

Section: Discussionsupporting

confidence: 86%

Is HERV-K and HERV-W Expression Regulated by miR-155 in Kidney Transplant Patients with Human Cytomegalovirus Infection?

et al. 2018

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According to the latest update, 2,578 unique mature microRNAs (miRNAs) are currently annotated in the human genome and participate in the regulation of multiple events, such as cellular proliferation or apoptosis. A previous study analyzing global miRNA expression patterns in GH cells (high human endogenous retrovirus, HERV, K vs. low) showed that 2 miRNAs (miR-663 and miR-638) are differentially regulated and exhibit expression parallel to that of HERV-K. The aim of this study was to evaluate HERV-K and -W pol gene and miR-155 expression in kidney transplant recipients and the possible relationship between them. The comparison between kidney transplant patients negative for human cytomegalovirus (HCMV) infection and positive patients showed a significant difference in terms of miR-155 expression (p = 0.0111). We demonstrated that HERV-K and -W pol gene expression was significantly higher in CMV-infected kidney transplant recipients versus those not infected as previously reported by our groups. Our correlation data suggest that miR-155 are not directly involved in regulating the HERV notwithstanding that we together observed increased expression of HERV-K and -W and diminished expression of miR-155 in HCMV-infected human kidney transplant recipients.

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Section: Discussionsupporting

confidence: 86%

Is HERV-K and HERV-W Expression Regulated by miR-155 in Kidney Transplant Patients with Human Cytomegalovirus Infection?

et al. 2018

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“…The primers and probe target a highly conserved part of the 5′ untranslated region [26]. The primers for internal control, i.e., glyceraldehyde-3-phosphate dehydrogenase (GAPDH), have been previously published [30]. …”

Section: Methodsmentioning

confidence: 99%

“…The total volume of the reaction mixture was 50 μL. The RT reaction was carried out at 42°C for 15 min, followed by 99°C for 5 min and then 5°C for 5 min [30]. …”

Section: Methodsmentioning

confidence: 99%

Aichivirus in Children with Diarrhea in Northern Italy

Bergallo¹,

Galliano²,

Montanari³

et al. 2017

Intervirology

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Objective: Since its discovery, Aichivirus (AiV) A has been detected, with an incidence of 0.9–4.1%, primarily when studying outbreaks of diarrhea in children or young adults. In this paper, we report the first detection of AiV in Piedmont, Italy, in pediatric patients. Methods: A total of 159 fecal specimens (from 96 males and 63 females) previously screened for rotaviruses, adenoviruses, noroviruses, human parechoviruses, saliviruses, and sapoviruses were collected from infants and children with acute gastroenteritis. Results: The most commonly detected virus was norovirus GII (33.80%), fol lowed by rotavirus (21.30%), astrovirus (18.87%), boca virus (13.92%), sapovirus (10.90%), parechovirus (8%), norovirus GI (6.70%), adenovirus (1%), and salivirus (0.52%). Real-time polymerase chain reaction detected AiV A in 1 (0.62%) case subjects. AiV A was detected in monoinfection only in January. Conclusions: Our results indicate that AiV may be associated with a limited number of diarrhea cases in pediatric patients.

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“…To test the presence of contamination or the generation of nonspecific amplification products, control reactions lacking the template (“no template” controls) were included in each run. The presence of cellular GAPDH was analyzed as an internal control; the primer sequences for the housekeeping gene have been previously described [25]. …”

Section: Methodsmentioning

confidence: 99%

Combining Real-Time COLD- and MAMA-PCR TaqMan Techniques to Detect and Quantify R201 <b><i>GNAS</i></b> Mutations in the McCune-Albright Syndrome

et al. 2017

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Background/Aim: The McCune-Albright syndrome (MAS) is a potentially severe disorder hallmarked by fibrous bone dysplasia, café-au-lait skin spots, and endocrine hyperfunction. It is caused by postzygotic activating mutations at the R201 codon of the GNAS gene, leading to a state of somatic mosaicism. Our aim was to improve the mutation detection rate and to quantify the presence of R201 GNAS mutations in different DNA samples from MAS patients. Methods: Real-time COLD- and MAMA-PCR TaqMan techniques were combined to search for R201 mutations in the DNA of blood or affected tissues from 16 previously molecularly characterized MAS patients, from a further 84 subjects with MAS signs who were R201 negative at RFLP analysis, and from 36 controls. The ability of this new method to provide quantitative data was tested in the serial dilution of wild-type, R201H, or R201C cloned plasmid DNA samples; the mutant abundance was measured by spectrophotometry. Results: A linear correlation between the true and the relative mutant abundance was observed until 2.5%, indicating a reliable quantification of R201 mutations. The assay’s sensitivity was 0.05%, similar to that of previously described molecular methods. Conclusion: The real-time COLD-MAMA-PCR approach is a rapid, efficient, and inexpensive molecular technique for the identification of mutant alleles poorly represented in DNA samples.

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CMV induces HERV-K and HERV-W expression in kidney transplant recipients

Cited by 51 publications

References 13 publications

Is HERV-K and HERV-W Expression Regulated by miR-155 in Kidney Transplant Patients with Human Cytomegalovirus Infection?

Is HERV-K and HERV-W Expression Regulated by miR-155 in Kidney Transplant Patients with Human Cytomegalovirus Infection?

Aichivirus in Children with Diarrhea in Northern Italy

Combining Real-Time COLD- and MAMA-PCR TaqMan Techniques to Detect and Quantify R201 <b><i>GNAS</i></b> Mutations in the McCune-Albright Syndrome

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