CMV infection of liver transplant recipients: comparison of antigenemia and molecular biology assays

Amorim, Marta; Cabeda, José Manuel; Seca, R.; Mendes, Ana Constança; Castro, Ana Paula Beltran Moschione; Amorim, José Manuel

doi:10.1186/1471-2334-1-2

Cited by 18 publications

(12 citation statements)

References 19 publications

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“…It represents the centroid of the latter. ment (transplant recipients) [Pellegrin et al, 2000;Amorim et al, 2001;Kulkarni et al, 2001;Hebart et al, 2002]. However, the present report is the first concurrent quantitative real-time detection of CMV DNA by TaqMan method, pp65 antigenemia, and pp67 RNA assay.…”

Section: Discussionmentioning

confidence: 79%

Assessment of CMV load in solid organ transplant recipients by pp65 antigenemia and real‐time quantitative DNA PCR assay: Correlation with pp67 RNA detection

Mengoli

Cusinato

Biasolo

et al. 2004

Journal of Medical Virology

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After bone marrow (BM) or solid-organ (SO) transplantation viremic Cytomegalovirus (CMV) infection is observed frequently. Quantitative assay of CMV in blood helps the management of this clinical condition. In the present report, 83 samples from 39 solid organ recipients, three CMV assays were compared simultaneously for the first time: the Nuclisens CMV pp67 assay (nucleic acid sequence-based amplification, NASBA), an "in-house" quantitative real-time PCR assay (TaqMan) for CMV DNA, and pp65 antigenemia. The relation between CMV DNA and pp65 antigenemia, the quantitative assays, was evaluated on a larger group including 251 blood samples from 118 solid organ recipients. Real-time PCR provided the best results; > or =130 CMV DNA copies/2 x 10(5) peripheral blood leukocytes (PBLs) predicted > or =1 pp65 antigen positive (Ag+) cell/2 x 10(5) PBLs. By taking pp65 antigenemia as the "gold standard," the sensitivity of CMV DNA quantitation and of the pp67 RNA assay were 0.95 and 0.20, respectively, while the corresponding specificity values were 0.50 and 0.93. When real-time PCR was considered as the "gold standard," the sensitivity and specificity of the pp65 antigenemia were 0.65 and 0.91, respectively. Among the three tests examined, the sensitivity of the pp67 RNA assay was the lowest. On the other hand, the pp67 RNA assay was highly specific and effective in pinpointing high viremia patients. The present report, by providing predictive values for all three diagnostic profiles, DNA load, antigenemia, and pp67RNA, is a contribution for validation of real-time PCR as a new standard for quantitative assessment of CMV viremia in clinical settings.

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Section: Discussionmentioning

confidence: 79%

Assessment of CMV load in solid organ transplant recipients by pp65 antigenemia and real‐time quantitative DNA PCR assay: Correlation with pp67 RNA detection

Mengoli

Cusinato

Biasolo

et al. 2004

Journal of Medical Virology

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“…In immunocompromised patients, especially after organ transplantation, rapid, sensitive and reliable detection of HCMV infection is necessary (Amorim et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

In situ detection of DNA and mRNA of human cytomegalovirus to distinguish different forms of viral infection in leukocytes.

Zawilińska¹,

Bulek²,

Kopec³

et al. 2006

Acta Biochim Pol

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In situ PCR and in situ reverse transcription PCR (RT-PCR) were applied to discriminate between latent and productive infection of human cytomegalovirus (HCMV) in leukocytes. We investigated 28 samples, in which viral pp65 antigen was detected only in the cytoplasm of leukocytes. Additionally we assayed 12 specimens lacking pp65 antigen. Using nested PCR (nPCR), viral DNA was detected in 27 samples. In six samples the results of nPCR were unreadable due to the presence of polymerase inhibitors. By application of in situ PCR, we were able to confirm the presence of viral DNA in the nucleus and/or cytoplasm. Productive infection was recognized in 20 samples in which transcripts for late viral genes were detected. Among the 20 samples negative by in situ RT-PCR, we recognized phagocytosis of viral particles in eight and the latent form of HCMV infection in five.

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“…It is not suitable for routine monitoring of patients on treatment [57]…why not???. It is a quick (4-6 hours), specific and extremely sensitive test but false positives may result from contamination during the test run.…”

Section: Polymerase Chain Reaction-pcrmentioning

confidence: 99%

“…It is fast, direct and sensitive, and is considered a quantitative technique for viral load [54,55]. CMV antigenemia is one of the earlier tests with positive results [17,25,[51][52][53][54][55][56][57][58] and becomes positive on average 9-18 days before establishment of the disease. It has been widely used for the early detection of active infection in organ transplant recipients [17,24,25,36,56].…”

Section: Immunofluorescence (If)mentioning

confidence: 99%

Cytomegalovirus Infection in Liver Transplantation

Sampaio¹,

Guardia²,

Milan³

et al. 2013

Manifestations of Cytomegalovirus Infection

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CMV infection of liver transplant recipients: comparison of antigenemia and molecular biology assays

Cited by 18 publications

References 19 publications

Assessment of CMV load in solid organ transplant recipients by pp65 antigenemia and real‐time quantitative DNA PCR assay: Correlation with pp67 RNA detection

Assessment of CMV load in solid organ transplant recipients by pp65 antigenemia and real‐time quantitative DNA PCR assay: Correlation with pp67 RNA detection

In situ detection of DNA and mRNA of human cytomegalovirus to distinguish different forms of viral infection in leukocytes.

Cytomegalovirus Infection in Liver Transplantation

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