CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to <i>fusarium oxysporum</i> infection in <i>Chrysanthemum morifolium</i>

Miao, Weihao; Xiao, Xiangyu; Wang, Yuean; Ge, Lijiao; Yang, Yanrong; Liu, Ye; Yuan, Ling; Guan, Zhiyong; Chen, Sumei; Fang, Weimin; Chen, Fadi; Zhao, Shuang

doi:10.1093/hr/uhad101

Cited by 12 publications

(9 citation statements)

References 58 publications

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“…Additionally, 13 AP2-EREBP and 14 WRKY TFs were detected specifically in JA-I, which may play important positive regulatory roles in mediating the JA signal pathway against A. alternata in chrysanthemum. Our recent research shows that CmWRKY6 negatively regulates the resistance to Fusarium oxysporum [ 39 ], and in the present study, WRKY6 was upregulated in both JA-I vs. JA and MOCK-I vs. MOCK groups, and the CmWRKY6 overexpression line had reduced susceptibility to black spot disease compared to the control. Differences in pathogenicity between these reports might be because F. oxysporum is a soilborne plant pathogen whose hypha penetrates plant roots rather than invading leaves like A. alternata .…”

Section: Discussionsupporting

confidence: 73%

“…To further verify the reliability of the results, we choose WRKY6 (evm.TU.scaffold_9505.8), which was induced by A. alternata , to generate overexpressed and silenced (RNA interference [RNAi]) CmWRKY6 chrysanthemum plants [ 39 ]. Inoculation assays demonstrated that compared with wild-type (WT), the CmWRKY6 overexpressing (OX-CmWRKY6) lines had enhanced resistance to black spot disease, with a lesion area that was reduced by 55%.…”

Section: Resultsmentioning

confidence: 99%

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Jasmonate signaling drives defense responses against Alternaria alternata in chrysanthemum

Zhang,

Miao,

Liu

et al. 2023

BMC Genomics

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Background Black spot disease caused by the necrotrophic fungus Alternaria spp. is one of the most devastating diseases affecting Chrysanthemum morifolium. There is currently no effective way to prevent chrysanthemum black spot. Results We revealed that pre-treatment of chrysanthemum leaves with the methy jasmonate (MeJA) significantly reduces their susceptibility to Alternaria alternata. To understand how MeJA treatment induces resistance, we monitored the dynamics of metabolites and the transcriptome in leaves after MeJA treatment following A. alternata infection. JA signaling affected the resistance of plants to pathogens through cell wall modification, Ca2+ regulation, reactive oxygen species (ROS) regulation, mitogen‐activated protein kinase cascade and hormonal signaling processes, and the accumulation of anti-fungal and anti-oxidant metabolites. Furthermore, the expression of genes associated with these functions was verified by reverse transcription quantitative PCR and transgenic assays. Conclusion Our findings indicate that MeJA pre-treatment could be a potential orchestrator of a broad-spectrum defense response that may help establish an ecologically friendly pest control strategy and offer a promising way of priming plants to induce defense responses against A. alternata.

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Section: Discussionsupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

Jasmonate signaling drives defense responses against Alternaria alternata in chrysanthemum

Zhang,

Miao,

Liu

et al. 2023

BMC Genomics

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“…tomato DC3000 (Fang et al, 2021). CmWRKY6‐1 negatively regulates resistance to Fusarium oxysporum , whereas CmWRKY15‐like positively regulates resistance to Fusarium oxysporum in chrysanthemums (Miao et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

A pattern for the early, middle, and late phase of tea chrysanthemum response to Fusarium oxysporum

Li,

Wang,

Liu

et al. 2024

Physiologia Plantarum

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Chrysanthemum morifolium is cultivated worldwide and has high ornamental, tea, and medicinal value. With the increasing area of chrysanthemum cultivation and years of continuous cropping, Fusarium wilt disease frequently occurs in various production areas, seriously affecting the quality and yield and causing huge economic losses. However, the molecular response mechanism of Fusarium wilt infection remains unclear, which limits the molecular breeding process for disease resistance in chrysanthemums. In the present study, we analyzed the molecular response mechanisms of ‘Huangju,’ one of the tea chrysanthemum cultivars severely infested with Fusarium wilt in the field at the early, middle, and late phases of F. oxysporum infestation. ‘Huangju’ responded to the infestation mainly through galactose metabolism, plant‐pathogen interaction, auxin, abscisic acid, and ethylene signalling in the early phase; galactose metabolism, plant‐pathogen interaction, auxin, salicylic acid signal, and certain transcription factors (e.g., CmWRKY48) in the middle phase; and galactose metabolism in the late phase. Notably, the galactose metabolism was important in the early, middle, and late phases of ‘Huangju’ response to F. oxysporum. Meanwhile, the phytohormone auxin was involved in the early and middle responses. Furthermore, silencing of CmWRKY48 in ‘Huangju’ resulted in resistance to F. oxysporum. Our results revealed a new molecular pattern for chrysanthemum in response to Fusarium wilt in the early, middle, and late phases, providing a foundation for the molecular breeding of chrysanthemum for disease resistance.

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“…Transcription factors (TFs) families, such as WRKY and NAC, also contribute to immune responses [ 27 ]. For instance, CmWRKY6-1 and CmWRKY8-1 could enhance resistance to Fusarium oxysporum in chrysanthemum [ 28 , 29 ], and NACL-D1 improves the resistance to Fusarium wilt in wheat [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancing cowpea wilt resistance: insights from gene coexpression network analysis with exogenous melatonin treatment

Gan,

Tu,

Yang

et al. 2024

BMC Plant Biol

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Background Cowpea wilt is a harmful disease caused by Fusarium oxysporum, leading to substantial losses in cowpea production. Melatonin reportedly regulates plant immunity to pathogens; however the specific regulatory mechanism underlying the protective effect of melatonin pretreated of cowpea against Fusarium oxysporum remains known. Accordingly, the study sought to evaluate changes in the physiological and biochemical indices of cowpea following melatonin treated to facilitate Fusarium oxysporum resistance and elucidate the associated molecular mechanism using a weighted gene coexpression network. Results Treatment with 100 µM melatonin was effective in increasing cowpea resistance to Fusarium oxysporum. Glutathione peroxidase (GSH-PX), catalase (CAT), and salicylic acid (SA) levels were significantly upregulated, and hydrogen peroxide (H2O2) levels were significantly downregulated in melatonin treated samples in roots. Weighted gene coexpression network analysis of melatonin- and Fusarium oxysporum-treated samples identified six expression modules comprising 2266 genes; the number of genes per module ranged from 9 to 895. In particular, 17 redox genes and 32 transcription factors within the blue module formed a complex interconnected expression network. KEGG analysis revealed that the associated pathways were enriched in secondary metabolism, peroxisomes, phenylalanine metabolism, flavonoids, and flavonol biosynthesis. More specifically, genes involved in lignin synthesis, catalase, superoxide dismutase, and peroxidase were upregulated. Additionally, exogenous melatonin induced activation of transcription factors, such as WRKY and MYB. Conclusions The study elucidated changes in the expression of genes associated with the response of cowpea to Fusarium oxysporum under melatonin treated. Specifically, multiple defence mechanisms were initiated to improve cowpea resistance to Fusarium oxysporum.

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CmWRKY6–1–CmWRKY15-like transcriptional cascade negatively regulates the resistance to fusarium oxysporum infection in Chrysanthemum morifolium

Cited by 12 publications

References 58 publications

Jasmonate signaling drives defense responses against Alternaria alternata in chrysanthemum

Jasmonate signaling drives defense responses against Alternaria alternata in chrysanthemum

A pattern for the early, middle, and late phase of tea chrysanthemum response to Fusarium oxysporum

Enhancing cowpea wilt resistance: insights from gene coexpression network analysis with exogenous melatonin treatment

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