2013
DOI: 10.1302/2046-3758.212.2000216
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Co-administration of hyaluronic acid with local anaesthetics shows lower cytotoxicity than local anaesthetic treatment alone in bovine articular chondrocytes

Abstract: ObjectiveTo study the effect of hyaluronic acid (HA) on local anaesthetic chondrotoxicity in vitro.MethodsChondrocytes were harvested from bovine femoral condyle cartilage and isolated using collagenase-containing media. At 24 hours after seeding 15 000 cells per well onto a 96-well plate, chondrocytes were treated with media (DMEM/F12 + ITS), PBS, 1:1 lidocaine (2%):PBS, 1:1 bupivacaine (0.5%):PBS, 1:1 lidocaine (2%):HA, 1:1 bupivacaine (0. 5%):HA, or 1:1 HA:PBS for one hour. Following treatment, groups had c… Show more

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Cited by 14 publications
(13 citation statements)
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“…Similar to our previous study involving OA chondrocyte cultures, co-treatment with HA did not decrease chondrotoxicity caused by CAR (Euppayo et al, 2015), triamcinolone acetonide (Euppayo et al, 2016), dexamethasone (Siengdee et al, 2015) and prednisolone (Siengdee et al, 2015). However, some studies have shown that HA could reduce cytotoxicity on chondrocyte viability after cells were exposed to bupivacaine (Onur, Sitron & Dang, 2013), indomethacin (Hashizume & Mihara, 2009), dexamethasone (Hashizume & Mihara, 2009) and celecoxib (Hashizume & Mihara, 2009). These results might have been caused by the specific properties associated with the molecular weight of HA.…”
Section: Discussionsupporting
confidence: 84%
“…Similar to our previous study involving OA chondrocyte cultures, co-treatment with HA did not decrease chondrotoxicity caused by CAR (Euppayo et al, 2015), triamcinolone acetonide (Euppayo et al, 2016), dexamethasone (Siengdee et al, 2015) and prednisolone (Siengdee et al, 2015). However, some studies have shown that HA could reduce cytotoxicity on chondrocyte viability after cells were exposed to bupivacaine (Onur, Sitron & Dang, 2013), indomethacin (Hashizume & Mihara, 2009), dexamethasone (Hashizume & Mihara, 2009) and celecoxib (Hashizume & Mihara, 2009). These results might have been caused by the specific properties associated with the molecular weight of HA.…”
Section: Discussionsupporting
confidence: 84%
“…Hyaluronan is an essential component of the extracellular matrix and is involved in important physiological functions, such as cell-to-cell identification, cellular cohesion, growth regulation, and antioxidant capacity [14] . Its combination with LAs may also ameliorate apoptotic cell death and enhance cell survival in chondrocytes [15,16] . However, the precise mechanisms of hyaluronan actions, especially those associated with cytoprotection, have not been firmly established, despite its widespread use in outpatient clinics.…”
Section: Wwwchinapharcom Lee Yj Et Almentioning
confidence: 99%
“…7 Finally, the association of HA with local anesthetics reduced their cytotoxicity with bupivacaine but not lidocaine, suggesting different mechanisms of injury between the two compounds. 8 Esterification of steroids, producing crystals of various sizes, is another structural option that makes them water insoluble, slowly degraded by the synovial esterase enzymes, with a delayed absorption and a more prolonged local antiinflammatory effect. 9 The sodium-phosphate derivatives on the contrary, are clear, hydrophilic injectable fluids, with a short acting, but quick anti-inflammatory action.…”
Section: Resultsmentioning
confidence: 99%