Co- and distinct existence of Tris-NTA and biotin functionalities on individual and adjacent micropatterned surfaces generated by photo-destruction

Abstract

Micropatterned surfaces with Tris-NTA and biotin functionalities both in the same micropattern as well as individually in adjacent micropatterns are generated by UV light illumination through photo-masks. These surfaces are extremely useful for the immobilization of oligohistidine and biotin tagged multiple biomolecules/proteins.

Fluorescence fluctuation of an antigen–antibody complex: circular dichroism, FCS and smFRET of enhanced GFP and its antibody

“…The binding efficiency of anti-EGFP V H H-His 6 with its antigen (EGFP) was studied using our previously developed micropatterned surface chemistry. 57 The antigen, EGFP-Mal3 (without histidine tag), binds strongly and specifically to the antibody anti-EGFP V H H-His 6 immobilized on a Tris-NTA square shaped micropatterned surface as is indicated by the intense green colour (Fig. 1).…”