Co-circulation of influenza A(H1N1), A(H3N2), B(Yamagata) and B(Victoria) during the 2017−2018 influenza season in Zhejiang Province, China

Mao, Haiyan; Sun, Yi; Chen, Yin; Fang, Zhiguo; Zhao, Yu; Wang, Xinying; Ding, Zheyuan; Cheng, Wei; Zhang, Dan; Zhang, Yanjun; Jiang, Jianmin

doi:10.1017/s0950268820000412

Cited by 6 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the influenza A/H3N2, the previous reports found R277Q and D69N at the antigenic epitope C, N137K, and N187K at the antigenic epitope D and E78K/G at the antigenic epitope E of the HA [ 60 , 61 ]. Among these, four amino acid substitutions (N137K, N187K, I422V, and G500E) belong to clade 3C.2a.1 and are represented by A/Singapore/INFIMH-16-0019/2016(H3N2) virus [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Genome characterization and mutation analysis of human influenza A virus in Thailand

et al. 2022

View full text Add to dashboard Cite

The influenza A viruses have high mutation rates and cause a serious health problem worldwide. Therefore, this study focused on genome characterization of the viruses isolated from Thai patients based on the next-generation sequencing technology. The nasal swabs were collected from patients with influenza-like illness in Thailand during 2017-2018. Then, the influenza A viruses were detected by reverse transcription-quantitative polymerase chain reaction and isolated by MDCK cells. The viral genomes were amplified and sequenced by Illumina MiSeq platform. Whole genome sequences were used for characterization, phylogenetic construction, mutation analysis and nucleotide diversity of the viruses. The result revealed that 90 samples were positive for the viruses including 44 of A/H1N1 and 46 of A/H3N2. Among these, 43 samples were successfully isolated and then the viral genomes of 25 samples were completely amplified. Finally, 17 whole genomes of the viruses (A/H1N1, n=12 and A/H3N2, n=5) were successfully sequenced with an average of 232,578 mapped reads and 1,720 genome coverage per sample. Phylogenetic analysis demonstrated that the A/H1N1 viruses were distinguishable from the recommended vaccine strains. However, the A/H3N2 viruses from this study were closely related to the recommended vaccine strains. The nonsynonymous mutations were found in all genes of both viruses, especially in HA and NA genes. The nucleotide diversity analysis revealed negative selection in the PB1, PA, HA, and NA genes of the A/H1N1 viruses. High-throughput data in this study allow for genetic characterization of circulating influenza viruses which would be crucial for preparation against pandemic and epidemic outbreaks in the future.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome characterization and mutation analysis of human influenza A virus in Thailand

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The number of severe cases was also higher. [30][31][32] Therefore, there was an urgent need to study the pathogenicity and transmissibility of the major influenza viruses circulating in 2018.…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity and transmissibility assessment of two strains of human influenza virus isolated in China in 2018

et al. 2021

View full text Add to dashboard Cite

Objective Influenza season occurs every year in China, but its presentation was unusual in the period from December 2017 to early 2018. During this period, influenza activity was increasing across the country and was much greater than during the same period in previous years, with great harm to people’s health. Methods In this study, we isolated two human influenza virus strains—A/Hebei/F076/2018(H1N1) and B/Hebei/16275B/2018—from patients with severe influenza in Hebei, China, during the flu season in January 2018, and explored their genetic characteristics, pathogenicity, and transmissibility. Results A/Hebei/F076/2018(H1N1) belongs to the human-like H1N1 influenza virus lineage, whereas B/Hebei/16275B/2018 belongs to the Victoria lineage and is closely related to the World Health Organization reference strain B/Brisbane/60/2008. Pathogenicity tests revealed that A/Hebei/F076/2018(H1N1) replicated much more strongly in mice, with mice exhibiting 40% mortality, whereas B/Hebei/16275B/2018 was not lethal. Both viruses could be transmitted through direct contact and by the aerosol route between guinea pigs, but the H1N1 strain exhibited higher airborne transmissibility. Conclusions These results may contribute to the monitoring of influenza mutation and the prevention of an influenza outbreak.

show abstract

“…In the influenza A/H3N2, the previous reports found R277Q and D69N at the antigenic epitope C, N137K and N187K at the antigenic epitope D and E78K/G at the antigenic epitope E of the HA (141,142). Among these, four amino acid substitutions (N137K, N187K, I422V and G500E) belong to clade 3C.2a.1 and are represented by A/Singapore/INFIMH-16-0019/2016(H3N2) virus (143).…”

Section: Discussionmentioning

confidence: 98%

Analysis of influenza a genomes and bacterial microbiota in upper respiratory tracts of influenza patients

Rattanaburi

View full text Add to dashboard Cite

Seasonal influenza is a contagious respiratory illness caused by influenza viruses that infect the upper respiratory tract (URT) of humans and represent a major burden for public health. Furthermore, the URT is colonized by a diverse microbial community which is a key factor protective from pathogens and may directly or indirectly affect viral infection. Therefore, the first aim of this study focuses on influenza A genome characterization and mutation analysis based on NGS technology. The phylogenetic analysis based on the deduced amino acid sequences revealed that the recommended vaccine (A/H1N1) strain might be less effective, whereas the recommended vaccine (A/H3N2) was more effective against the circulating influenza viruses in Thailand during 2017-2018. In addition, several nonsynonymous mutations occurred across eight segmented genes of both viruses, particularly in HA and NA genes. Indeed, nucleotide diversity analysis was observed negative selection in the PB1, PA, HA, and NA genes of A/H1N1 viruses. Then, the second aim of this study is to compare the bacterial microbiota profile in the URT with influenza (Flu A or Flu B groups) and non-influenza (COVID-19 or Non-Flu & COVID-19) patients by 16S rDNA sequencing. The Shannon diversity for the influenza group was significantly lower than Non-Flu & COVID-19 group. The beta diversity revealed that microbial compositions were significantly different among groups. The relative abundance showed that the family of Enterobacteriaceae was increased the in influenza group, whereas Streptococcus, Prevotella, Veillonella, and Fusobacterium were predominated in Non-Flu & COVID-19. In summary, our data provide fundamental knowledge to investigate the association host-microbe interaction that might be useful for predicting health status and applied for microbiome engineering to enhance immunity system to future infections.

show abstract

Co-circulation of influenza A(H1N1), A(H3N2), B(Yamagata) and B(Victoria) during the 2017−2018 influenza season in Zhejiang Province, China

Cited by 6 publications

References 8 publications

Genome characterization and mutation analysis of human influenza A virus in Thailand

Genome characterization and mutation analysis of human influenza A virus in Thailand

Pathogenicity and transmissibility assessment of two strains of human influenza virus isolated in China in 2018

Analysis of influenza a genomes and bacterial microbiota in upper respiratory tracts of influenza patients

Contact Info

Product

Resources

About