In China, chestnut burrs (CB) are produced at a rate of a million tons per year as the major byproduct of chestnut orchards. It is necessary to utilize the chestnut forest green waste and convert it into a valuable seedling media for the sustainable cultivation of chestnut seedlings. In this study, we composted CB with two waste products of cattle farming, namely cow manure (CM) and bovine bone (BM). We also evaluated the potential of CB compost application in chestnut forest sustainability. Results indicated that the best combination was the addition of 15% BM and 55% CM. This combination significantly improved the composting environment by increasing pH, enhancing phosphorus concentration and mineral elements such as Ca, Na, Mg and Zn, and shortened the composting period to 38 days. This combination also resulted in the highest content of citric acid-P (109.20 times than the control treatment) and the lowest content of NH 4 + -N (0.28 times than control treatment) indicating a better N and P structure of the final compost product. This combination achieved a greater degradation rate of CB cellulose (61.45%), hemicellulose (37.87%), and a more significant degradation of outer epidermis structure. When CB compost was used as a growing media, a significant decrease in photosynthesis stress of chestnut seedlings was observed, which was mainly manifested as a decrease in photochemical quenching (qP) and an increase of the maximum efficiency of PSII photochemistry under dark-adaption (Fv/Fm). Addition of 10% CB compost (in volume basis) is suggested, which resulted in the tallest chestnut seedlings (59.83 cm) with a stem diameter of 0.91 cm after six months of growth. In summary, this research provides an environmentally friendly strategy for chestnut orchard sustainability: rapid composting of CB, then immediate application as a high-quality substrate for chestnut seedlings.Agronomy 2020, 10, 288 2 of 18 step for chestnut production. The popularization of burr removal equipment has resulted in the need for intensive management of CB. Due to CB recalcitrant biodegradability and knotty spicular structure, incineration is still the main method of dealing with CB waste [3,4]. Since CB, as FGW, is rich in organic matter and minerals, it is desirable to convert it into a high value byproduct such as compost.Composting is a popular and clean method to dispose of FGW [5]. Mature FGW compost has high porosity and high organic carbon content and can be utilized as a high-quality soil amendment [6]. Shrestha et al. [7] reported that the application of compost increased soil organic carbon content, reduced CO 2 and N 2 O emissions, and increased CH 4 uptake. Chehab et al. [8] indicated that the olive FGW compost amendment resulted in an increased performance in mature olive trees and in a maximum yield of photosystem II (Fv/Fm). Composting recycles organic matter, nitrogen, phosphorous, and mineral elements in the FGW when growing seedlings [9]. In a recent study, Shalizi et al. [10] increased the biomass of Eucalyptus bentham...