Co–Cr–Mo alloy binding peptide as molecular glue for constructing biomedical surfaces

Abstract

The mechanical properties of Co–Cr–Mo (CCM) alloys are advantageous in various biomedical applications. However, because of their bioinert surface, CCM alloys exhibit poor endothelial cell attachment properties; thus, problems of biocompatibility remain. In this study, we aimed to improve the biocompatibility of the CCM alloy surface using solid-binding peptides. We selected peptides with high binding affinity for cast CCM alloy surfaces through in vitro evolution by the phage display method. The peptides were functionalized on the CCM alloy surfaces by simple immersion in the peptide solution. The peptide bound to both cast and 3D-printed CCMs with the same affinity. The peptides linked to the amino acid motif that promotes cell adhesion, and improved the attachment of endothelial cells on the 3D-printed CCM in serum and serum-free conditions. Hence, CCM-binding peptides are attractive tools for constructing a biofunctional surface on CCM-based biodevices.

Binding stability of peptides to Co–Cr–Mo alloy affects proliferation and differentiation of osteoblast

“…We selected a CBP with the sequence QHKYTPIHEGRW, which was found via phage display in a previous study (Migita et al, 2020). The CBP-RGD peptide, which is CBP ligated with Arg-Gly-Asp-Ser (RGDS), was chemically synthesized to a purity exceeding 90% by Eurofins Genomics Inc.…”

“…(Ceylan et al, 2011; Müller et al, 2005; Oya et al, 2009; Sakamoto & Haruyama, 2010). The solid binding peptide is beneficial for immobilizing the biofunctional molecules onto a specific metal such as Ti, Au, or SUS316L (Care et al, 2015; Khatayevich et al, 2010; Migita et al, 2020; Sakaguchi‐Mikami et al, 2020; Sano & Shiba, 2003). In the study of metallic biomaterials, several novel alloys are being created to improve biocompatibility.…”

“…Therefore, RGD was chemically linked to CBP. The chimeric peptide CBP-RGD provided a good scaffolding ability on the alloy surface for endothelial cells (Migita et al, 2020). However, the stability of the peptide under biological conditions is still unclear.…”

“…Hence, it is important to identify the solid binding peptide for novel biometals that are used as structural materials, such as Co, Ta, Zr, Nb, W, and their alloys. In a previous study, we identified a CCM binding peptide (CBP) (Migita et al, 2020). Some integrins, that are cell membrane proteins, bind to the cell‐adhesive motif Arg–Gly–Asp (RGD), which is derived from fibronectin (Hautanen et al, 1989), and facilitate the attachment of cells to the substrate upon their interaction (Main et al, 1992).…”

Biomedical Alloys and Physical Surface Modifications: A Mini-Review

“…Trimble et al [ 54 ] developed a finite element model to predict the orthogonal forces of biomedical grade Co–Cr–Mo alloy and to reduce the number of machining tests. Migita et al [ 55 ] used solid-binding peptides to improve the biocompatibility of the Co–Cr–Mo alloys. Yamanaka et al [ 56 ] prepared Co–Cr–Mo alloy rods with a small diameter by hot-caliber rolling which exhibited high strength and durability.…”