Co-culture microorganisms with different initial proportions reveal the mechanism of chalcopyrite bioleaching coupling with microbial community succession

Ma, Liyuan; Wang, Xingjie; Feng, Xue; Liang, Yili; Xiao, Yunhua; Hao, Xiaodong; Yin, Huaqun; Liu, Hongwei; Liu, Xueduan

doi:10.1016/j.biortech.2016.10.056

Cited by 68 publications

(25 citation statements)

References 37 publications

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“…Also noteworthy is the effect of microorganisms to mineral bioleaching. The microbiology of leaching environments-including physiology of the most common community members, microbial successions [7,58], the relationship of the population dynamics with environmental factors [59,60], and the influence of community composition on ecosystem functioning [61,62]-have always been the target for research on bioleaching processes and mechanisms. Next generation sequencing technology enables the comprehensive analysis of genomes, which can recover information about their general characteristics, especially their metabolic potential [3,63].…”

Section: Ngs For Genome Analysismentioning

confidence: 99%

“…Metals are the metabolic requirements for microorganisms when they maintain the proper concentrations, but beyond certain concentrations they become toxic to the microorganism, mainly as a result of their ability to denature protein molecules [2]. However, A huge microbial diversity with wide metabolic potential that is influenced both by interactions with other bacteria and with the variable environment exists in most bioleching systems [7,10]. To elucidate the functional response of microbial communities to changing environmental conditions A huge microbial diversity with wide metabolic potential that is influenced both by interactions with other bacteria and with the variable environment exists in most bioleching systems [7,10].…”

Section: Introductionmentioning

confidence: 99%

“…However, A huge microbial diversity with wide metabolic potential that is influenced both by interactions with other bacteria and with the variable environment exists in most bioleching systems [7,10]. To elucidate the functional response of microbial communities to changing environmental conditions A huge microbial diversity with wide metabolic potential that is influenced both by interactions with other bacteria and with the variable environment exists in most bioleching systems [7,10]. To elucidate the functional response of microbial communities to changing environmental conditions has been challenging [11,12].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Bioleaching Microbial Community Structure and Function Based on Next-Generation Sequencing Technologies

et al. 2018

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It is widely known that bioleaching microorganisms have to cope with the complex extreme environment in which microbial ecology relating to community structure and function varies across environmental types. However, analyses of microbial ecology of bioleaching bacteria is still a challenge. To address this challenge, numerous technologies have been developed. In recent years, high-throughput sequencing technologies enabling comprehensive sequencing analysis of cellular RNA and DNA within the reach of most laboratories have been added to the toolbox of microbial ecology. The next-generation sequencing technology allowing processing DNA sequences can produce available draft genomic sequences of more bioleaching bacteria, which provides the opportunity to predict models of genetic and metabolic potential of bioleaching bacteria and ultimately deepens our understanding of bioleaching microorganism. High-throughput sequencing that focuses on targeted phylogenetic marker 16S rRNA has been effectively applied to characterize the community diversity in an ore leaching environment. RNA-seq, another application of high-throughput sequencing to profile RNA, can be for both mapping and quantifying transcriptome and has demonstrated a high efficiency in quantifying the changing expression level of each transcript under different conditions. It has been demonstrated as a powerful tool for dissecting the relationship between genotype and phenotype, leading to interpreting functional elements of the genome and revealing molecular mechanisms of adaption. This review aims to describe the high-throughput sequencing approach for bioleaching environmental microorganisms, particularly focusing on its application associated with challenges.

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Section: Ngs For Genome Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of Bioleaching Microbial Community Structure and Function Based on Next-Generation Sequencing Technologies

et al. 2018

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“…Chalcopyrite (CuFeS 2 ) accounts for ∼70% of the Earth's copper and is one of the most wide-spread copper sulfide minerals (Lu et al, 2016). However, the Cu extraction from chalcopyrite through acid leaching is an extremely timeconsuming process with low efficiency (Ma et al, 2017). Therefore, it has greater practical significance and value to study introduction mechanisms in the leaching system of low-grade chalcopyrite with indigenous leaching microbial communities.…”

Section: Introductionmentioning

confidence: 99%

Effect of Introduction of Exogenous Strain Acidithiobacillus thiooxidans A01 on Structure and Function of Adsorbed and Planktonic Microbial Consortia During Bioleaching of Low-Grade Copper Sulfide

et al. 2020

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The introduction of Acidithiobacillus thiooxidans A01 strengthens the positive interactions between physiologically distinct microorganisms and enhances the bioleaching ability of the consortium. However, the effect of introducing an exogenous strain, A. thiooxidans A01 on the structure and function of the adsorbed and planktonic microbial consortia during bioleaching of low-grade copper sulfide remains unclear. In this study, A. thiooxidans A01 was introduced into an indigenous leaching microbial community on the 0th (group B), 24th (group C), and 36th day (group D). Results revealed that the copper leaching efficiency was highest in group D, in which the Cu 2+ concentration in the solution reached 251.5 mg/L on day 48, which was 18.5% higher than that of the control (group A, no addition of A. thiooxidans A01). Restriction fragment length polymorphism (RFLP) analysis of the microbial community in group D revealed the presence of Leptospirillum ferriphilum, Acidithiobacillus ferrooxidans, Acidithiobacillus caldus, Sulfobacillus sp., Acidiphilium spp., and Acidithiobacillus albertensis before introduction of A. thiooxidans A01 on the 36th or 48th day; however, A. albertensis was absent on day 48 in group A. Further, the proportion of dominant A. caldus, L. ferriphilum, and A. ferrooxidans became altered. The results of real-time PCR in group D showed that A. thiooxidans A01 was primarily adsorbed on the surface of the ore, with the adsorption reaching the maxima on day 42; while the free A. thiooxidans A01 in solution grew slowly, reaching its maximum concentration on day 45. Compared with that in the control group, the abundance of both free and attached A. caldus and Sulfobacillus sp. decreased following the introduction of A. thiooxidans A01, while that of L ferriphilum, A. ferrooxidans, and Acidiphilium sp. increased. Functional gene arrays data indicated that the abundance of genes involved in sulfide and iron oxidation in L. ferriphilum and A. ferrooxidans, as well as that of the metal (loid) resistance genes of

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“…Four moderately thermophilic strains (Leptospirillum ferriphilum DX, Acidithiobacillus caldus DX, Sulfobacillus thermosulfidooxidans ST and Ferroplasma thermophilum L1) used in this study were isolated from an acid mine drainage in China [19][20][21]. Each strain grew in basal salt media at 45 • C except that L. ferriphilum DX subcultured at 40 • C. L. ferriphilum DX grew at an initial pH of 1.6 and supplemented with FeSO 4 ·7H 2 O (44.7 g/L); A. caldus DX was maintained with an initial pH of 2.0 and added to S 0 (10 g/L); S. thermosulfidooxidans ST was at an initial pH of 1.6 and supplemented with FeSO 4 ·7H 2 O (44.7 g/L) and 0.02% (wt/vol) yeast extract; and F. thermophilum L1 grew in basal salt medium at an initial pH of 1.0 and added with FeSO 4 ·7H 2 O (44.7 g/L) and 0.01% (wt/vol) yeast extract.…”

Section: Preparation Of the Mixed Moderate Thermophilesmentioning

confidence: 99%

Carbon Material with High Specific Surface Area Improves Complex Copper Ores’ Bioleaching Efficiency by Mixed Moderate Thermophiles

et al. 2018

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Abstract:The catalysis of carbon materials with different specific surface areas (SSA) (2, 400, 800 and 1200 m 2 /g) on complex copper ores bioleaching by moderately mixed thermophiles was investigated. The copper extractions increased with the rise in SSA of carbon materials. A recovery of 98.8% copper in the presence of 1200 m 2 /g activated carbon was achieved, and improved by 30.7% and 76.4% compared with biotic control and chemical leaching. Moreover, the addition of 1200 m 2 /g activated carbon adsorbed large amount of bacteria, accelerated the oxidation rate of ferrous iron and maintained the solution redox potential at relatively low values, and significantly increased the dissolution of primary copper sulfide (62.7%) compared to biotic control (6.0%). Microbial community succession revealed that activated carbon changed the microbial community composition dramatically. The S. thermosulfidooxidans ST strain gained a competitive advantage and dominated the microbial community through the whole bioleaching process. The promoting effect of carbon material with higher SSA on copper extraction was mainly attributed to better galvanic interaction, biofilm formation, direct contact and lower redox potential.

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Co-culture microorganisms with different initial proportions reveal the mechanism of chalcopyrite bioleaching coupling with microbial community succession

Cited by 68 publications

References 37 publications

Assessment of Bioleaching Microbial Community Structure and Function Based on Next-Generation Sequencing Technologies

Assessment of Bioleaching Microbial Community Structure and Function Based on Next-Generation Sequencing Technologies

Effect of Introduction of Exogenous Strain Acidithiobacillus thiooxidans A01 on Structure and Function of Adsorbed and Planktonic Microbial Consortia During Bioleaching of Low-Grade Copper Sulfide

Carbon Material with High Specific Surface Area Improves Complex Copper Ores’ Bioleaching Efficiency by Mixed Moderate Thermophiles

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