Co-culture of annulus fibrosus cells and bone marrow mesenchymal stem cells

Xu, Yuan; Zhang, X J; Fang, Lijun; Tianjiao, Zhao,

doi:10.4238/2015.april.27.7

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…Second, anabolic, anti-inflammatory, and anti-apoptotic properties have been attributed to the implanted MSCs [13] . In vitro coculture experiments demonstrated stimulatory effects of MSCs on the proliferation and extracellular matrix synthesis of nucleus pulposus (NP) and annulus fibrous (AF) cells [14] , [15] . Furthermore, MSCs supplementation was effective in suppressing pro-inflammatory markers in degenerative and inflammatory cell and tissue culture models [16] , [17] .…”

Section: Introductionmentioning

confidence: 99%

Intervertebral disc response to stem cell treatment is conditioned by disc state and cell carrier: An ex vivo study

Peroglio

Douma

Caprez

et al. 2017

Journal of Orthopaedic Translation

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SummaryIn vitro and in vivo studies evidenced that mesenchymal stem cells (MSCs) contribute to intervertebral disc (IVD) regeneration by differentiation towards the disc phenotype and matrix synthesis and/or by paracrine signalling to endogenous cells, thereby promoting a healthier disc phenotype in degenerative discs. The aim of this study was to investigate IVD response to human MSC (hMSC) treatment based on the disc degenerative state and hMSC carrier. Bovine caudal IVDs with endplates were cultured in a bioreactor under simulated physiological (0.1 Hz load and sufficient glucose) or degenerative (10 Hz load and limited glucose) conditions for 7 days. Discs were partially nucleotomised, restored with hMSCs in either fibrin gel or saline solution and cultured under physiological conditions for 7 days. Controls included fibrin and saline without hMSCs. Cell viability, histology, disc height, and gene expression analyses were performed to evaluate regeneration. hMSCs in fibrin were viable and homogenously distributed following 7 days of culture under dynamic loading in partially nucleotomised discs. IVD response to hMSCs was conditioned by both disc degenerative state and hMSC carrier. The effect of the regenerative treatment was stronger on simulated-degenerative discs than on simulated-physiological discs. hMSCs in fibrin induced a superior anabolic response in degenerative IVDs compared with fibrin alone, thus suggesting an added value of the cellular therapy compared with an acellular solution. When comparing fibrin and saline as a hMSC carrier, a significantly higher anabolic response was observed in IVDs treated with hMSCs in fibrin. Moreover, it was found that the degenerative state of the disc influenced hMSC differentiation. Indeed, a significantly higher expression of specific discogenic markers (ACAN and CA12) was observed in hMSCs implanted into physiological discs than in those implanted into degenerative discs. In conclusion, host disc cells and donor hMSC response depend on the degenerative state of the host disc and carrier used for hMSC delivery, and these two aspects need to be considered for a successful translation of hMSC therapies for the treatment of IVD degeneration.

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Section: Introductionmentioning

confidence: 99%

Intervertebral disc response to stem cell treatment is conditioned by disc state and cell carrier: An ex vivo study

Peroglio

Douma

Caprez

et al. 2017

Journal of Orthopaedic Translation

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“…Cell–cell interactions are important roles in cell proliferation and differentiation of MSCs. Combinations of annulus fibrosus cells with BMSCs enhanced somatic cell proliferation and extracellular matrix synthesis [ 45 ]. When stem cells were co-implanted with somatic functional cells, cell number of both cell types increased and promoted tissue regeneration in vivo [ 46 ].…”

Section: Optimal Cell Source For Cell Therapymentioning

confidence: 99%

Strategies to Optimize Adult Stem Cell Therapy for Tissue Regeneration

Liu

Zhou

Zhang

et al. 2016

IJMS

130

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Stem cell therapy aims to replace damaged or aged cells with healthy functioning cells in congenital defects, tissue injuries, autoimmune disorders, and neurogenic degenerative diseases. Among various types of stem cells, adult stem cells (i.e., tissue-specific stem cells) commit to becoming the functional cells from their tissue of origin. These cells are the most commonly used in cell-based therapy since they do not confer risk of teratomas, do not require fetal stem cell maneuvers and thus are free of ethical concerns, and they confer low immunogenicity (even if allogenous). The goal of this review is to summarize the current state of the art and advances in using stem cell therapy for tissue repair in solid organs. Here we address key factors in cell preparation, such as the source of adult stem cells, optimal cell types for implantation (universal mesenchymal stem cells vs. tissue-specific stem cells, or induced vs. non-induced stem cells), early or late passages of stem cells, stem cells with endogenous or exogenous growth factors, preconditioning of stem cells (hypoxia, growth factors, or conditioned medium), using various controlled release systems to deliver growth factors with hydrogels or microspheres to provide apposite interactions of stem cells and their niche. We also review several approaches of cell delivery that affect the outcomes of cell therapy, including the appropriate routes of cell administration (systemic, intravenous, or intraperitoneal vs. local administration), timing for cell therapy (immediate vs. a few days after injury), single injection of a large number of cells vs. multiple smaller injections, a single site for injection vs. multiple sites and use of rodents vs. larger animal models. Future directions of stem cell-based therapies are also discussed to guide potential clinical applications.

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Role of Inflammatory Niche and Adult Cardiomyocyte Coculture on Differentiation, Matrix Synthesis, and Secretome Release by Human Bone Marrow Mesenchymal Stem Cells

Joshi

Kothapalli

2022

Appl Biochem Biotechnol

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Co-culture of annulus fibrosus cells and bone marrow mesenchymal stem cells

Cited by 6 publications

References 18 publications

Intervertebral disc response to stem cell treatment is conditioned by disc state and cell carrier: An ex vivo study

Intervertebral disc response to stem cell treatment is conditioned by disc state and cell carrier: An ex vivo study

Strategies to Optimize Adult Stem Cell Therapy for Tissue Regeneration

Role of Inflammatory Niche and Adult Cardiomyocyte Coculture on Differentiation, Matrix Synthesis, and Secretome Release by Human Bone Marrow Mesenchymal Stem Cells

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