Co-culture of ASCs/EPCs and dermal extracellular matrix hydrogel enhances the repair of full-thickness skin wound by promoting angiogenesis

Abstract

Background The repair of large-scale full-thickness skin defects represents a challenging obstacle in skin tissue engineering. To address the most important problem in skin defect repair, namely insufficient blood supply, this study aimed to find a method that could promote the formation of vascularized skin tissue. Method The phenotypes of ASCs and EPCs were identified respectively, and ASCs/EPCs were co-cultured in vitro to detect the expression of dermal and angiogenic genes. Furthermore, the co-culture system combined with dermal extracellular matrix hydrogel was used to repair the full-scale skin defects in rats. Result The co-culture of ASCs/EPCs could increase skin- and angiogenesis-related gene expression in vitro. The results of in vivo animal experiments demonstrated that the ASCs/EPCs group could significantly accelerate the repair of skin defects by promoting the regeneration of vascularized skin. Conclusion It is feasible to replace traditional single-seed cells with the ASC/EPC co-culture system for vascularized skin regeneration. This system could ultimately enable clinicians to better repair the full-thickness skin defects and avoid donor site morbidity.

Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction

“…The SMBC was cut into 2 cm diameter disks and stored at 4 °C. Subsequently, we digested and counted ADSCs that grew to 70–80% and added them to the scaffold material at a concentration of 1 × 10 6 cell/ml [ 18 , 19 ]. The medium was changed every 2–3 days for tissue-engineered urethra and the composite was used as a graft for urethral defect repair after 7 days.…”

Application of Tilapia Skin Acellular Dermal Matrix to Induce Acute Skin Wound Repair in Rats

“…The repair process not only requires a large number of fibroblasts to proliferate, but also the formation of blood vessels and nerves is equally important. Large area full-thickness skin repair, due to the lack of vascularization of new skin, affecting the effect of repair, resulting in slow wound healing, obvious wound contraction and the formation of obvious scar tissue ( Lin et al, 2021 ). Appropriate ECM structure can provide structural support for the growth and migration of fibroblasts, and combine with it to promote the expression of related factors and promote wound healing ( Xue et al, 2021 ).…”

Aerobic exercise-induced circulating extracellular vesicle combined decellularized dermal matrix hydrogel facilitates diabetic wound healing by promoting angiogenesis

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