Co-culture of Bacillus amyloliquefaciens and recombinant Pichia pastoris for utilizing kitchen waste to produce fengycins

Miao, Chang-Hao; Qiao, Bin; Xu, Shujing; Cheng, Jing-Sheng

doi:10.1016/j.jbiosc.2022.02.009

Cited by 16 publications

(8 citation statements)

References 42 publications

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“…Fengycin has different isoforms. Fengycin B is not the same as fengycin A in that the d -Ala at the sixth position in the circular peptide is replaced by d -Val . Fengycin C differs from fengycin B in that threonine is present instead of tyrosine at the ninth position of the cyclic peptide .…”

Section: Resultsmentioning

confidence: 97%

“…Fengycin B is not the same as fengycin A in that the D-Ala at the sixth position in the circular peptide is replaced by D-Val. 53 Fengycin C differs from fengycin B in that threonine is present instead of tyrosine at the ninth position of the cyclic peptide. 54 In this study, ions m/z 724.58, m/z 966, and m/z 1448.10 were detected, indicating the presence of (C15) fengycin A (Table S1).…”

Section: Structural Characterization Of Fengycin Produced By Recombin...mentioning

confidence: 99%

“…58 The samples were analyzed by mass spectrometry using an electrospray quadrupole time-of-flight mass spectrometer (ESI-Q-TOF, model MicrOTOF-QII) with an ESI ion source, voltage of 4.5 kV, drying gas temperature of 180 °C, flow rate of 6 mL/min, positive ion full scan mode, and a scan range of 50−2000 m/z. 53 3.6. Antifungal Activity Assay.…”

Section: Extraction and Identification Of Fengycinmentioning

confidence: 99%

“…The extraction of fengycin was performed as previously described. 53 The fermentation broth was centrifuged, subsequently acidified to pH 2 with 6 M HCl, and placed at 4 °C for 12 h. The resulting precipitate was collected by centrifugation, lyophilized, and extracted with methanol. Methanol was removed with a rotary evaporator and fengycin was dissolved with chromatographic grade methanol.…”

Section: Extraction and Identification Of Fengycinmentioning

confidence: 99%

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Improved Production of Fengycin in Bacillus subtilis by Integrated Strain Engineering Strategy

et al. 2022

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Fengycin is a lipopeptide with broad-spectrum antifungal activity. However, its low yield limits its commercial application. Therefore, we iteratively edited multiple target genes associated with fengycin synthesis by combinatorial metabolic engineering. The ability of Bacillus subtilis 168 to manufacture lipopeptides was restored, and the fengycin titer was 1.81 mg/L. Fengycin production was further increased to 174.63 mg/L after knocking out pathways associated with surfactin and bacillaene synthesis and replacing the native promoter (P ppsA ) with the P veg promoter. Subsequently, fengycin levels were elevated to 258.52 mg/L by upregulating the expression of relevant genes involved in the fatty acid pathway. After blocking spore and biofilm formation, fengycin production reached 302.51 mg/L. Finally, fengycin production was increased to approximately 885.37 mg/L after adding threonine in the optimized culture medium, which was 488-fold higher compared with that of the initial strain. Integrated strain engineering provides a strategy to construct a system for improving fengycin production.

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Section: Resultsmentioning

confidence: 97%

Section: Structural Characterization Of Fengycin Produced By Recombin...mentioning

confidence: 99%

Section: Extraction and Identification Of Fengycinmentioning

confidence: 99%

Section: Extraction and Identification Of Fengycinmentioning

confidence: 99%

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Improved Production of Fengycin in Bacillus subtilis by Integrated Strain Engineering Strategy

et al. 2022

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“…Kitchen waste constitutes of 47.3 ± 0.6% starch, 25.0 ± 0.8% lipid and 6.8 ± 0.4% protein on dry weight basis. For kitchen waste pre-treatment, P. pastoris strains were designed for lipase, amylase and glucosidase expression and, respectively, 90 U.mL −1 , 385.4 U.mL −1 and 247.3 U.mL −1 enzyme activities were achieved [ 142 ]. Three engineered P. pastoris strains for secretion of hydrolytic enzymes were co-cultured with Bacillus amyloliquefaciens HM618 that is engineered to produce fengycin, and 6.6 times higher fengycin concentration was obtained from kitchen wastes compared to the production with pure culture [ 142 ] .…”

Section: Introductionmentioning

confidence: 99%

Second generation Pichia pastoris strain and bioprocess designs

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et al. 2022

Biotechnol Biofuels

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Yeast was the first microorganism used by mankind for biotransformation processes that laid the foundations of industrial biotechnology. In the last decade, Pichia pastoris has become the leading eukaryotic host organism for bioproduct generation. Most of the P. pastoris bioprocess operations has been relying on toxic methanol and glucose feed. In the actual bioeconomy era, for sustainable value-added bioproduct generation, non-conventional yeast P. pastoris bioprocess operations should be extended to low-cost and renewable substrates for large volume bio-based commodity productions. In this review, we evaluated the potential of P. pastoris for the establishment of circular bioeconomy due to its potential to generate industrially relevant bioproducts from renewable sources and waste streams in a cost-effective and environmentally friendly manner. Furthermore, we discussed challenges with the second generation P. pastoris platforms and propose novel insights for future perspectives. In this regard, potential of low cost substrate candidates, i.e., lignocellulosic biomass components, cereal by-products, sugar industry by-products molasses and sugarcane bagasse, high fructose syrup by-products, biodiesel industry by-product crude glycerol, kitchen waste and other agri-food industry by products were evaluated for P. pastoris cell growth promoting effects and recombinant protein production. Further metabolic pathway engineering of P. pastoris to construct renewable and low cost substrate utilization pathways was discussed. Although, second generation P. pastoris bioprocess operations for valorisation of wastes and by-products still in its infancy, rapidly emerging synthetic biology tools and metabolic engineering of P. pastoris will pave the way for more sustainable environment and bioeconomy. From environmental point of view, second generation bioprocess development is also important for waste recycling otherwise disposal of carbon-rich effluents creates environmental concerns. P. pastoris high tolerance to toxic contaminants found in lignocellulosic biomass hydrolysate and industrial waste effluent crude glycerol provides the yeast with advantages to extend its applications toward second generation P. pastoris strain design and bioprocess engineering, in the years to come. Graphical Abstract

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