Co-culture of early bovine embryos with oviductal epithelium

Beneficial effects of Vero cells for developing IVF bovine eggs in two different coculture systems

“…, cumulus cells (Kajihara et al, 1987), granulosa cells (Rexroad, 1989) or bovine oviductal epithelial cells (Gandolfi and Moor, 1987;Eyestone et al, 1987). All these feeder cells used in coculture were able to improve in vitro development of bovine zygotes compared to a medium devoid of cells.…”

Evaluation of laser-assisted lentiviral transgenesis in bovine

“…Culture of presumptive bovine zygotes was, until the 1980s, only possible up to the 8 -16 cell stage, where development stopped (Eyestone et al, 1987). This so called 8-cell block could be overcome by co-culture of the early embryos with bovine oviduct epithelial cells (Eyestone et al, 1987).…”

“…This so called 8-cell block could be overcome by co-culture of the early embryos with bovine oviduct epithelial cells (Eyestone et al, 1987). The development of cell free, conditioned media followed, where only the supernatant of cultured cells was used to cultivate bovine embryos to the blastocyst stage (Eyestone and First, 1989).…”

In vitro techniques of bovine oocyte maturation, fertilization and embryo culture resulting in the birth of a calf

“…The degenerated cells contained mostly electron-dense residual bodies of unknown origin. (Boland, 1984;Sirard et aL, 1985;Lu et al, 1987) or in coculture with oviductal or follicular cells (Eystone et al, 1987;Fuduka et aL, 1988;Lu et al, 1988;Thibault et aL, 1988 (Pavlok et al, 1988a Table I). …”

“…3) (Eystone et al, 1987;Lu et al, 1988;Fukui and Ono, 1988;Thibault, 1988), or with granulosa cells (Fukuda et al, 1988), the 8-cell cleavage block (Bavister, 1988) (Pivko et aL, 1986 Even if the present technique for culture of bovine embryos seems to be superior to culture methods described by the authors cited above and also apparently to the culture in sheep oviduct (Sirard et al, 1988), more effort is still necessary to obtain normal blastocysts under well-defined in vitro conditions and further research is required to understand the regulatory mechanisms of early embryonic development.…”