Co-culture of microalgae and enriched nitrifying bacteria for energy-efficient nitrification

Kwon, Gyutae; Kim, Hyeon; Song, Chul-Woo; Jahng, Deokjin

doi:10.1016/j.bej.2019.107385

Cited by 39 publications

(23 citation statements)

References 101 publications

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“…In this respect, NOB is more affected by light than AOB, which can make nitrite accumulate under light intensities higher than 225 μmol•m -2 •s -1 (Vergara et al, 2016). Furthermore, if oxygen is limiting, nitrite concentration can increase due to the faster oxygen consumption of AOB in comparison to NOB (Kwon et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Nitrite inhibition of microalgae induced by the competition between microalgae and nitrifying bacteria

et al. 2020

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Outdoor microalgae cultivation systems treating anaerobic membrane bioreactor (AnMBR) effluents usually present ammonium oxidising bacteria (AOB) competition with microalgae for ammonium uptake, which can cause nitrite accumulation. In literature, nitrite effects over microalgae have shown controversial results. The present study evaluates the nitrite inhibition role in a microalgae-nitrifying bacteria culture. For this purpose, pilot-and lab-scale assays were carried out. During the continuous outdoor operation of the membrane photobioreactor (MPBR) plant, biomass retention time (BRT) of 2 d favoured AOB activity, which caused nitrite accumulation. This nitrite was confirmed to inhibit microalgae performance. Specifically, continuous 5-d lab-scale assays showed a reduction in the nitrogen recovery efficiency by 32, 42 and 80% when nitrite concentration in the culture accounted for 5, 10 and 20 mg N•L-1 , respectively. On the contrary, short 30-min exposure to nitrite showed no significant differences in 2 the photosynthetic activity of microalgae under nitrite concentrations of 0, 5, 10 and 20 mg N•L-1. On the other hand, when the MPBR plant was operated at 2.5-d BRT, the nitrite concentration was reduced to negligible values due to increasing activity of microalgae and nitrite oxidising bacteria (NOB). This allowed obtaining maximum MPBR performance; i.e. nitrogen recovery rate (NRR) and biomass productivity of 19.7 ± 3.3 mg N•L-1 •d-1 and 139 ± 35 mg VSS•L-1 •d-1 , respectively; while nitrification rate (NOxR) reached the lowest value (13.5 ± 3.4 mg N•L-1 •d-1). Long BRT of 4.5 d favoured NOB growth, avoiding nitrite inhibition. However, it implied a decrease in microalgae growth and the accumulation of nitrate in the MPBR effluent. Hence, it seems that optimum BRT has to be within the range 2-4.5 d in order to favour microalgae growth with respect to AOB and NOB.

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Section: Introductionmentioning

confidence: 99%

Nitrite inhibition of microalgae induced by the competition between microalgae and nitrifying bacteria

et al. 2020

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“…Recently, the symbiotic process of microalgae and bacteria has been significantly used for the treatment of municipal and industrial wastewater [10,13]. The microalgae-bacteria consortia maintain cost-effective aeration, sequester the greenhouse gas CO 2 , and limit the risk of pollutant volatilization [14][15][16]. The interaction between microalgae and bacteria ensures efficient removal of organic and inorganic carbon, nitrogen, phosphorus, heavy metals, and other pollutants.…”

Section: Introductionmentioning

confidence: 99%

“…Due to this interaction, the organic matter mineralization by aerobic bacteria produces the inorganic carbon needed by the microalgae. In turn, the O 2 required for bacterial degradation is produced photosynthetically by the microalgae [14,15].…”

Section: Introductionmentioning

confidence: 99%

“…It was reported that the latter influences the average of biomass productivity, which can be increased by 21.6 ± 2.1% at the artificial light/dark cycle [17]. The few studies available, focused on the illumination cycle [14,15], have proved that the dark phase promotes the recovery of nitrifiers. Moreover, a study reported that the influence of light activity could inhibit ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB).…”

Section: Introductionmentioning

confidence: 99%

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Real-Time Behavior of a Microalgae–Bacteria Consortium Treating Wastewater in a Sequencing Batch Reactor in Response to Feeding Time and Agitation Mode

Mhedhbi

Khélifi

Foladori

et al. 2020

Water

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A study of a microalgae–bacteria treatment system was conducted in a sequencing batch reactor (SBR) by combining a precultured native algae Nannochloropsis gaditana L2 with spontaneous municipal wastewater microorganisms. Two types of agitation, air mixing (AI) and mechanical mixing (MIX), were assessed at continuous illumination (L) and photoperiod cycle light/dark (L/D). The obtained consortium, via native microalgae addition, has a better operational efficiency compared to spontaneous control. This allows the removal of 78% and 53% of total Kjeldhal nitrogen (TKN) and chemical oxygen demand (COD), respectively. Under the (L/D) photoperiod, the optimal removal rate (90% of TKN and 75% of COD) was obtained by the consortium at 4 days of hydraulic retention time (HRT) using the AI mode. Moreover, during feeding during dark (D/L) photoperiod, the highest removal rate (83% TKN and 82% COD) was recorded at 4 days HRT using the AI mode. These results bring, at the scale of a bioreactor, new data regarding the mode of aeration and the feeding time. They prove the concept of such a technology, increasing the attraction of microalgae-based wastewater treatment.

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“…To purify wastewater, nitrogen, and phosphorous must be removed; thus, the cultivation of microalgae using wastewater can be used to simultaneously purify wastewater and produce biomass at a low cost (Benítez et al, 2019;Cheng et al, 2019). Furthermore, bacteria can be cultured together with microalgae during cultivation with wastewater (Kwon et al, 2019). Depending on the characteristics of the bacteria being cultured, bacteria-derived floc can often form.…”

Section: Introductionmentioning

confidence: 99%

Effects of Co-culture on Improved Productivity and Bioresource for Microalgal Biomass Using the Floc-Forming Bacteria Melaminivora Jejuensis

Kim

Yun

Kim

et al. 2020

Front. Bioeng. Biotechnol.

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Bacterial and algal floc formation was induced by inoculating three species of wastewater-derived bacteria (Melaminivora jejuensis, Comamonas flocculans, and Escherichia coli) into algal cultures (Chlorella sorokiniana). Bacterial and algal flocs formed in algal cultures inoculated with M. jejuensis and C. flocculans, and these flocs showed higher sedimentation rates than pure algal culture. The floc formed by M. jejuensis (4988.46 ± 2589.81 μm) was 10-fold larger than the floc formed by C. flocculans (488.60 ± 226.22 μm), with a three-fold higher sedimentation rate (M. jejuensis, 91.08 ± 2.32% and C. flocculans, 32.55 ± 6.33%). Biomass and lipid productivity were improved with M. jejuensis inoculation [biomass, 102.25 ± 0.35 mg/(L·day) and 57.80 ± 0.20 mg/(L·day)] compared with the productivity obtained under pure algal culture conditions [biomass, 78.00 ± 3.89 mg/(L·day) and lipids, 42.26 ± 2.11 mg/(L·day)]. Furthermore, the fatty acid composition of the biomass produced under pure algal culture conditions was mainly composed of C16:0 (43.67%) and C18:2 (45.99%), whereas the fatty acid composition of the biomass produced by M. jejuensis was mainly C16:0 (31.80%), C16:1 (24.45%), C18:1 (20.23%), and C18:2 (16.11%). These results suggest the possibility of developing an efficient method for harvesting microalgae using M. jejuensis and provide information on how to improve biomass productivity using floc-forming bacteria.

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Co-culture of microalgae and enriched nitrifying bacteria for energy-efficient nitrification

Cited by 39 publications

References 101 publications

Nitrite inhibition of microalgae induced by the competition between microalgae and nitrifying bacteria

Nitrite inhibition of microalgae induced by the competition between microalgae and nitrifying bacteria

Real-Time Behavior of a Microalgae–Bacteria Consortium Treating Wastewater in a Sequencing Batch Reactor in Response to Feeding Time and Agitation Mode

Effects of Co-culture on Improved Productivity and Bioresource for Microalgal Biomass Using the Floc-Forming Bacteria Melaminivora Jejuensis

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