Co-delivery of ciprofloxacin and colistin using microcontainers for bacterial biofilm treatment

Birk, Stine Egebro; Mazzoni, Chiara; Javed, Madeeha M.; Hansen, Morten Borre; Johansen, Helle Krogh; Haagensen, Janus Anders Juul; Molin, Søren; Nielsen, Line Hagner

doi:10.1016/j.ijpharm.2021.120420

Cited by 5 publications

(6 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Each substrate chip, holding 100 microcontainers, was loaded with 1.0 ± 0.2 mg furosemide, corresponding to ≈10 µg per microcontainer. This was a substantially larger loading capacity than previously reported for similarly sized microdevices, which was 1.5 [ 4 ] and 3–5 µg per microdevice, [ 41 ] respectively. This higher loading capacity can be achieved since the structural sidewalls of the microdevices are thinner when fabricated by the custom‐built 3D printer.…”

Section: Resultsmentioning

confidence: 60%

3D‐Printed Radiopaque Microdevices with Enhanced Mucoadhesive Geometry for Oral Drug Delivery

Chang

Kjeldsen

Christfort

et al. 2022

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

During the past decades, microdevices have been evaluated as a means to overcome challenges within oral drug delivery, thus improving bioavailability. Fabrication of microdevices is often limited to planar or simple 3D designs. Therefore, this work explores how microscale stereolithography 3D printing can be used to fabricate radiopaque microcontainers with enhanced mucoadhesive geometries, which can enhance bioavailability by increasing gastrointestinal retention. Ex vivo force measurements suggest increased mucoadhesion of microcontainers with adhering features, such as pillars and arrows, compared to a neutral design. In vivo studies, utilizing planar X-ray imaging, show the time-dependent gastrointestinal location of microcontainers, whereas computed tomography scanning and cryogenic scanning electron microscopy reveal information about their spatial dynamics and mucosal interactions, respectively. For the first time, the effect of 3D microdevice modifications on gastrointestinal retention is traced in vivo, and the applied methods provide a much-needed approach for investigating the impact of device design on gastrointestinal retention.

show abstract

Section: Resultsmentioning

confidence: 60%

3D‐Printed Radiopaque Microdevices with Enhanced Mucoadhesive Geometry for Oral Drug Delivery

Chang

Kjeldsen

Christfort

et al. 2022

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

show abstract

“…The in vivo pharmacokinetic study presented trends towards delayed and sustained absorption of paracetamol from PCL microcontainers compared to powder in a gelatin capsule as control, with a time until maximum plasma concentration (Tmax) of 59 ± 31 min and 38 ± 26 min, respectively (11). Microcontainers have also been applied to confine amorphous indomethacin to lower the extent of crystallization, and they have shown promising preliminary results for oral delivery of cubosomes in relation to oral vaccine delivery and for eradication of bacterial biofilms (8,(15)(16)(17). Thus, many different applications for microcontainers in oral drug delivery are continuously explored.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo comparison of microcontainers and microspheres for oral drug delivery

Christfort

Strindberg

Al-khalili

et al. 2021

International Journal of Pharmaceutics

View full text Add to dashboard Cite

“…[18,[23][24][25][26][27] In a few cases, reservoir-based microdevices have been prepared for oral delivery of multiple drugs, however, without achieving sequential release, but merely simultaneous release of pharmaceutical compounds for therapeutical applications such as drug-resistant infections, diabetes, and cancer. [28][29][30] In one case, the sequential release was obtained from a flat microdevice with separate reservoirs of 5 𝜇m depth in an in vitro cell model, by loading different model drugs in hydrogel matrices using photolithography. However, the developed multi-reservoir microdevice was not evaluated in vivo.…”

Section: Introductionmentioning

confidence: 99%

Sequential Drug Release Achieved with Dual‐Compartment Microcontainers: Toward Combination Therapy

Christfort

Milián‐Guimerá

Kamguyan

et al. 2022

Advanced Therapeutics

Self Cite

View full text Add to dashboard Cite

Combination drug therapy is commonly used to treat cancer, diabetes, cardiovascular conditions, and infections. However, these therapies face challenges associated with patient compliance and toxicology. Over the past decades, microdevices have emerged as a promising candidate for oral delivery allowing for targeted drug delivery with a tunable drug release. In the present work, engineered and monodisperse dual‐compartment microdevices are developed to achieve a physical separation of two drugs followed by a sequential release in the gastrointestinal tract. As proof‐of‐concept, the compartments are sealed with two pH‐sensitive polymers of different thicknesses to control the sequential release of propranolol and furosemide. In vitro release studies and in vivo absorption studies in rats confirm a sequential drug release from the two compartments. Unlike other proposed approaches, it is highly advantageous that the drugs can be loaded directly as powders, and that their release can be tuned via optimized coatings to achieve the desired release and absorption profiles. Conclusively, this study lays a strong foundation for the future use of microdevices to enable co‐delivery of drugs followed by a sequential release in close proximity in the gastrointestinal tract.

show abstract

Co-delivery of ciprofloxacin and colistin using microcontainers for bacterial biofilm treatment

Cited by 5 publications

References 61 publications

3D‐Printed Radiopaque Microdevices with Enhanced Mucoadhesive Geometry for Oral Drug Delivery

3D‐Printed Radiopaque Microdevices with Enhanced Mucoadhesive Geometry for Oral Drug Delivery

In vitro and in vivo comparison of microcontainers and microspheres for oral drug delivery

Sequential Drug Release Achieved with Dual‐Compartment Microcontainers: Toward Combination Therapy

Contact Info

Product

Resources

About