Co-delivery of gentiopicroside and thymoquinone using electrospun m-PEG/PVP nanofibers: In-vitro and In vivo studies for antibacterial wound dressing in diabetic rats

Almukainzi, May; El‐Masry, Thanaa A.; Negm, Walaa A.; Elekhnawy, Engy; Saleh, Asmaa; Sayed, Ahmed E; Ahmed, Hytham M.; Abdelkader, Dalia H.

doi:10.1016/j.ijpharm.2022.122106

Cited by 32 publications

(20 citation statements)

References 57 publications

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“…The negative value might be attributed to the combinatory effect of PVP and GLR 48/18 [ 7 , 12 , 26 ]. The loading of CXB into the PVP matrix concurrently with micellar solubilization using GLR 48/16 could be an effective polymeric combination that efficiently entraps CXB leading to a higher drug loading capacity (0.198 mg of CXB per 10 mg of electrosprayed microparticles) [ 9 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…Generally, the IR spectrum of CXB-GLR-PVP MPs indicates that no chemical interaction occurs during micellar solubilization and formulation of electrosprayed CXB-GLR-PVP MPs [ 9 , 30 ]. The broadness of OH peaks could be attributed to possible H- bonding formation between CXB and PVP background [ 15 ]. Additionally, less intense peaks of CXB might indicate the enclosed entrapment of CXB into the PVP matrix [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…The broadness of OH peaks could be attributed to possible H- bonding formation between CXB and PVP background [ 15 ]. Additionally, less intense peaks of CXB might indicate the enclosed entrapment of CXB into the PVP matrix [ 15 ]. The peaks of CXB shown in the IR spectrum of the physical mixture indicate no possible interaction occurred with the active functional groups of PVP K30 and Gelurice ® 48/16.…”

Section: Discussionmentioning

confidence: 99%

“…PVP is a highly safe, water-soluble polymer, biocompatible. In addition, it is very soluble in a variety of organic solvents, making it a common ingredient in many pharmaceutical formulation processes [ 15 ]. It is widely employed in electrospinning/electrospraying to formulate nano-delivery systems due to its ideal properties of spinnability.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of Celecoxib PVP Microparticles Stabilized by Gelucire 48/16 via Electrospraying for Enhanced Anti-Inflammatory Action

et al. 2023

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Electrospraying (ES) technology is considered an efficient micro/nanoparticle fabrication technique with controlled dimensions and diverse morphology. Gelurice® 48/16 (GLR) has been employed to stabilize the aqueous dispersion of Celecoxib (CXB) for enhancing its solubility and oral bioavailability. Our formula is composed of CXB loaded in polyvinylpyllodine (PVP) stabilized with GLR to formulate microparticles (MPs) (CXB-GLR-PVP MPs). CXB-GLR-PVP MPs display excellent in vitro properties regarding particle size (548 ± 10.23 nm), zeta potential (−20.21 ± 2.45 mV), and drug loading (DL, 1.98 ± 0.059 mg per 10 mg MPs). CXB-GLR-PVP MPs showed a significant (p < 0.05) higher % cumulative release after ten minutes (50.31 ± 4.36) compared to free CXB (10.63 ± 2.89). CXB exhibited good dispersibility, proved by X-ray diffractometry (XRD), adequate compatibility of all components, confirmed by Fourier-Transform Infrared Spectroscopy (FTIR), and spherical geometry as revealed in scanning electron microscopy (SEM). Concerning our anti-inflammatory study, there was a significant decrease in the scores of the inflammatory markers’ immunostaining in the CXB-GLR-PVP MPs treated group. Also, the amounts of the oxidative stress biomarkers, as well as mRNA expression of interleukins (IL-1β and IL-6), considerably declined (p < 0.05) in CXB-GLR-PVP MPs treated group alongside an enhancement in the histological features was revealed. CXB-GLR-PVP MPs is an up-and-coming delivery system that could be elucidated in future clinical investigations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fabrication of Celecoxib PVP Microparticles Stabilized by Gelucire 48/16 via Electrospraying for Enhanced Anti-Inflammatory Action

et al. 2023

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“…Many genes in C. albicans are involved in biofilm development. Some of these genes encode transcription factors that function indirectly to control biofilm formation, such as BCR1 [ 40 , 41 ]. Others are related to adhesion, which is the first step in biofilm formation, such as surface adhesins ALS1 [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Silver Nanoparticles Prepared Using Encephalartos laurentianus De Wild Leaf Extract Have Inhibitory Activity against Candida albicans Clinical Isolates

Alherz

Negm

Elekhnawy

et al. 2022

JoF

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Candida albicans is a major human opportunistic pathogen causing infections, which range from cutaneous to invasive systemic infections. Herein, the antifungal and anti-biofilm potential of silver nanoparticles (AgNPs) green synthesized in the presence of Encephalartos laurentianus leaf extract (ELLE) were investigated. The bioactive chemicals of ELLE, including phenolics, flavonoids, and glycosides were identified and quantified for the first time. AgNPs showed minimum inhibitory concentration (MIC) values against C. albicans clinical isolates ranging from 8 to 256 µg/mL. In addition, AgNPs significantly decreased biofilm formation. The impact of AgNPs on the expression of the genes encoding biofilm formation was assessed using qRT-PCR. AgNPs had a beneficial role in the macroscopic wound healing, and they resulted in complete epithelization without any granulation tissue or inflammation. Treatment with AgNPs resulted in negative immunostaining of tumor necrosis factor-α. The levels of the inflammation markers, interleukin-6 and interleukin-1β, significantly decreased (p < 0.05) in the AgNPs-treated group. There was also a pronounced increase in the gene expression of fibronectin and platelet-derived growth factor in the wound tissues. Thus, AgNPs synthesized using ELLE may be a promising antifungal and wound healing agent.

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The Wound‐Healing Potential of the Endemic Plant Helianthemum canum (L.) Baumg: Preclinical Studies Supported with Phytochemical Profiling

Küpeli Akkol,

Kosar,

Baldemir

et al. 2023

Chemistry & Biodiversity

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The study's objective is to clarify the probable mechanisms underlying the wound‐healing properties of Helianthemum canum L. (Cistaceae), a traditional anti‐inflammatory and wound‐healing medicine. LC/MS‐MS was used to perform phytochemical analyses on a 70% methanol extract of the plant's aerial parts. In vivo linear incision and circular excision models were used to evaluate the wound healing activity. For anti‐inflammatory effect, in vivo acetic acid capillary permeability assay and in vitro Interleukin 1, Interleukin 6, and Interferon Ɣ levels in LPS‐induced FR skin fibroblast cell line were also evaluated. The extract significantly improved wound healing in experimental models, with tensile strength values of 27.8% and a contraction value of 35.09%. Histopathological examinations, hydroxyproline estimation, hyaluronidase, collagenase, and elastase enzyme inhibitory assays confirmed wound healing potential. Inflammatory cytokines were significantly inhibited in the LPS‐induced FR cell line, with the highest effect seen on IL‐6 (34.5±2.12 pg/mL). This study offered the first concrete proof that H. canum can be used to treat wounds by suggesting that the myricetin and quinic acid content identified by LCMS‐MS analysis may be accountable for the effect of H. canum on wound contraction and hydroxyproline production.

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Co-delivery of gentiopicroside and thymoquinone using electrospun m-PEG/PVP nanofibers: In-vitro and In vivo studies for antibacterial wound dressing in diabetic rats

Cited by 32 publications

References 57 publications

Fabrication of Celecoxib PVP Microparticles Stabilized by Gelucire 48/16 via Electrospraying for Enhanced Anti-Inflammatory Action

Fabrication of Celecoxib PVP Microparticles Stabilized by Gelucire 48/16 via Electrospraying for Enhanced Anti-Inflammatory Action

Silver Nanoparticles Prepared Using Encephalartos laurentianus De Wild Leaf Extract Have Inhibitory Activity against Candida albicans Clinical Isolates

The Wound‐Healing Potential of the Endemic Plant Helianthemum canum (L.) Baumg: Preclinical Studies Supported with Phytochemical Profiling

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