Co-digestion of Biowastes to Enhance Biological Hydrogen Process by Defined Mixed Bacterial Cultures

Abstract

Co-digestion of biowastes for hydrogen (H 2 ) production using defined mixed cultures can overcome the high risk of failure due to contamination and imbalanced nutrient status. H 2 production from biowastes-pea-shells, potato peels (PP), onion peels (OP) and apple pomace, either individually or in various combinations was evaluated by hydrolyzing with defined hydrolytic mixed bacterial culture (MHC5) and subjecting the hydrolysate to mixture of defined H 2 producers (MMC6). Co-digestion of OP and PP hydrolysate supplemented at H 2 production stage with GM-2 and M-9 media resulted in 95 and 102 l H 2 /kg of Total solids (TS), respectively compared to 84 l H 2 /kg of TS in control. Upscaling the process by digesting 4.0 l slurry (16-fold) resulted in 88.5 and 95 l H 2 /kg of TS, respectively compared to 72 l H 2 /kg of TS in control. Thus, H 2 production by co-digestion of biowastes could be improved through the supplementation with very dilute medium (0.1 9) and selection of suitable biowastes under unsterile conditions. The overall efficiency can be further enhanced by integrating it with bioprocesses for biopolymers such as polyhydroxyalkanoates and or biofuels like methane production.

Overview on the Current Status of Hydrogen Energy Research and Development in India

“…Various research activities brought up the new technologies for the gasification of biomass substances like sugarcane wastes, food wastes, paper wastes, polyethylene and wood chips, wastewater sludge, and branches of palm oil trees. Also, to enhance the rate of hydrogen production of biomass, various combinations were defined by hydrolyzing the biowaste like onion peels and potato peels with mixed bacterial cultures . Similarly, Fig.…”

“…Also, to enhance the rate of hydrogen production of biomass, various combinations were defined by hydrolyzing the biowaste like onion peels and potato peels with mixed bacterial cultures [29]. Similarly, Fig.…”

Production and Optimization of Cyclodextrin Glucanotranferase From Bacillus Sp. Ss2

“…The phylogenetic analysis of isolate SS2 carried out using MEGA X software shows that the strain clustered with the different species of Bacillus genus such as Bacillus cereus, Bacillus luti, Bacillus albus, Bacillus mycoides, and Bacillus tropicus (Figure 2). Genus Bacillus is known for its biotechnological applications such as hydrolytic enzymes as well as value added products (Kumar et al, 2013;Patel et al, 2019;Porwal et al, 2008).…”

Epigenetic Regulation of Gut Microbial Dysbiosis

“…The microbial colonization initiates from birth and maintains throughout the life span [1,2]. Various studies have demonstrated important roles of microorganisms in diverse fields, such as fermentation [3,4], biomolecules [5,6], bioremediation [7][8][9][10], anti-toxicity [11,12], and diseases and health [13][14][15][16][17][18]. Pathogenesis, including the recent outbreak of coronavirus, can be effectively treated through the application of these bioactive molecules, vaccines, and nanomaterials [11,14,[19][20][21][22][23][24].…”