This study evaluated rheological behavior of the pig waste biotransformation process to produce Lactic Acid (LA) and biomass with Lactobacillus acidophilus in a stirred reactor. Also, cell growth, carbohydrate consumption, and LA production at three different agitation speeds, 100, 150, and 200 rpm at 37°C, with a reaction time of 52 h. During the development of the process, the kinetic and rheological parameters were obtained using the logistic, Gompertz, and generalized Gompertz, Ostwald de Waele, and Herschel-Bulkley mathematical models, respectively. The substrate used was pig manure, to which molasses was added at 12% v/v to increase the concentration of carbohydrates. The results suggest that mass exchange is favorable at low agitation speeds. Still, the presence of molasses rich in carbohydrates as a carbon source modi es the characteristics of the uid, dilatant (n > 1) at the beginning of the process to end up as pseudoplastic (n < 1) due to the addition of exopolysaccharides and the modi cation of the physical structure of the substrate. This effect was con rmed by the Herschel-Bulkley model, which presented a better t to the data obtained, in addition to nding a direct relationship between viscosity and pH that can be used as variables for the control of bioconversion processes of pig manure into biomass rich in Lactobacillus acidophilus.