Co-exposure to commercial food product ingredient E341 and E551 triggers cytotoxicity in human mesenchymal stem cells

Athinarayanan, Jegan; Khaibary, Amjad A. L.; Periasamy, Vaiyapuri Subbarayan; Alatiah, Khalid A.; Shamlan, Ghalia; Alshatwi, Ali A.

doi:10.1007/s11356-022-24497-1

Cited by 4 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result of disrupted intracellular signaling and regulation, intracellular DNA is damaged, cellular death, mitochondrial physiology is impaired, and cell cycle arrest occurs. 25,[49][50] Moreover, molecular signaling is adversely affected. 51,52 A flavin-containing monooxygenase (FMO) is involved in oxidation-reduction metabolism, specifically xeno-oxidation.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication of myristic acid–potato starch complex nanostructures and assessment of their cytotoxic behavior

Almushawah,

Athinarayanan,

Periasamy

et al. 2023

J Sci Food Agric

Self Cite

View full text Add to dashboard Cite

BACKGROUNDLipids and carbohydrates play essential functions in foods. In the last few decades, food scientists have studied the effects of carbohydrate‐lipid interaction on food's functional properties. However, how carbohydrate‐lipid complex‐derived materials affect the biological system is unknown. We created a myristic acid‐potato starch complex using a simple cooking approach. The complex was employed as a precursor for myristic acid‐potato starch complex‐based nanostructured materials (MPS‐NMs) fabrication through a liquid‐liquid extraction approach. A study was conducted on the structural and cytotoxic features of fabricated MPS‐NMs.RESULTSThe transmission electron microscopy images confirm spherical nanostructure formation with 3 ‐ 60 nm in size. After 24 h exposure, the chloroform fraction‐ and n‐hexane fraction‐based MPS‐NMs enhanced cell death by ~ 90% and ~ 82%, respectively. The chloroform fraction‐based MPS‐NMs (CMPS‐NMs) triggers apoptotic cell death in human mesenchymal stem cells (hMSCs). Also, n‐hexane fraction‐based MPS‐NMs (HMPS‐NMs) treated cells have red colour‐intact nuclei, attributed to necrotic cell death. CMPS‐NMs and HMPS‐NMs significantly decreased the mitochondria membrane potential and increased intracellular ROS levels. We observed significant downregulation in FMO, ATM and UGT gene expression levels in the exposed cells of CMPS‐NMs and HMPS‐ NMs. In addition, GSR and GSTA4 genes have upregulation in CMPS‐NMs and HMPS‐ NMs exposure.CONCLUSIONOur results conclude that the cooking process may lead to the formation of nanostructured material in food systems. These CMPS‐NMs and HMPS‐NMs may also contribute to cell metabolic disorders.This article is protected by copyright. All rights reserved.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Cell‐death pathways are directly linked to affected redox mechanisms by external factors. As a result of disrupted intracellular signaling and regulation, intracellular DNA is damaged, cellular death, mitochondrial physiology is impaired, and cell cycle arrest occurs 25,49‐50 . Moreover, molecular signaling is adversely affected 51,52 .…”

Section: Resultsmentioning

confidence: 99%

Fabrication of myristic acid–potato starch complex nanostructures and assessment of their cytotoxic behavior

Almushawah,

Athinarayanan,

Periasamy

et al. 2023

J Sci Food Agric

Self Cite

View full text Add to dashboard Cite

show abstract

“…Intracellular ROS Level Measurement: In WSMC-NMs and MSMC-NMs untreated and treated hMSCs, the study examined the generation of intracellular reactive oxygen species utilizing 2,7-dichlorofluorescein diacetate (DCFH-DA). [41,42] In addition, the ROS combined with DCFH-DA results in the fluorescent substance dichlorofluorescein (DCF). The study seeded 40 000 cells into each well of 24-well plates.…”

Section: Characterization Of Starch-myristic Acid Complexmentioning

confidence: 99%

Morphological, Thermal, and Cytotoxicity Features Assessment of Starch–Myristic Acid Complex‐Derived Nanostructured Materials

Almushawah,

Periasamy,

Athinarayanan

et al. 2023

Starch Stärke

Self Cite

View full text Add to dashboard Cite

The present study investigates the morphological and cytotoxic properties of starch‐myristic acid complex (SMC) derivatives. We attempted to prepare a SMC by frying. By liquid‐liquid extraction, starch – myristic acid complex nanostructured materials were isolated. X‐ray diffractometers and transmission electron microscopes were used to determine the morphology and crystallization of the extractive. We performed various assays to assess the toxicological profile of starch‐myristic acid complex‐derived nanostructured materials (SMC‐NMs), including cellular viability tests, cellular and nuclear morphological examinations, mitochondrial membrane potential (MMP), and intracellular reactive oxygen species (ROS) measurements. Transmission electron microscopy (TEM) images reveal nanoscale particles between 30 and 90 nm in water fraction‐based SMC‐NMs (WSMC‐NMs) and 160 nm and 250 nm in methanol fraction‐based SMC‐NMs (MSMC‐NMs). The WSMC‐NMs and MSMC‐NMs decreased the cell viability by 77 and 91% at 250 μg/mL, respectively. The SMC‐NMs enhanced necrotic cell death by increasing the level of ROS in human mesenchymal stem cells (hMSCs). The SMC‐NMs altered gene expression within 24 hours. Gene expression of GSTM3 and GSR has explicitly upregulated in a dose‐dependent manner in WSMC‐NMs and MSMC‐NMs exposed cells. Our study found that nanoscale particles may develop during cooking, increasing the risk of diabetes, cardiovascular diseases, and obesity.This article is protected by copyright. All rights reserved

show abstract

Carbon Quantum Dots in Palmyra Palm Jaggery: Evaluation of Antioxidative and Cytocompatibility Features

Athinarayanan,

Periasamy,

Alshatwi

2024

Sugar Tech

View full text Add to dashboard Cite

Co-exposure to commercial food product ingredient E341 and E551 triggers cytotoxicity in human mesenchymal stem cells

Cited by 4 publications

References 27 publications

Fabrication of myristic acid–potato starch complex nanostructures and assessment of their cytotoxic behavior

Fabrication of myristic acid–potato starch complex nanostructures and assessment of their cytotoxic behavior

Morphological, Thermal, and Cytotoxicity Features Assessment of Starch–Myristic Acid Complex‐Derived Nanostructured Materials

Carbon Quantum Dots in Palmyra Palm Jaggery: Evaluation of Antioxidative and Cytocompatibility Features

Contact Info

Product

Resources

About