“…assessing splicing events between sexes, within treatments), and (2) how do males and females differ in their response to stress (i.e., assessing splicing events within the sexes, between treatments). We did not include analyses between different tissues (e.g., male gonads to female gonads, or male pituitary to male hypothalamus) as numerous other studies have identified tissue-specific splicing events; these comparisons would not aid in discerning unique splicing patterns between sexes, or between treatments (Grosso et al, 2008;Saha et al, 2017;Pineda & Bradley, 2018). Whippet identifies splicing events by type, including Core Exon (CE), Alternate Acceptor (AA), Alternate Donor (AD), Retained Intron (RI), Tandem Alternative Polyadenylation Site (TE), Tandem Transcription Start Site (TS), Alternative First Exon (AF), and Alternative Last Exon (AL).…”