Co‐Expression of Cytokeratins and Vimentin in Sheep Cumulus‐Oocyte Complexes. Alteration of Intermediate Filament Distribution by Acrylamide

Gall, Laurence; Smedt, Véronique De; Ruffini, Sylvie

doi:10.1111/j.1440-169x.1992.00579.x

Cited by 19 publications

(12 citation statements)

References 21 publications

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“…A functional relationship can, however, be excluded with a fair degree of certainty. (1) When CK filaments are destroyed in cultured cumulus cells by treating them with acrylamide, the isolated oocyte resumes meiosis (Gall et al 1992). …”

Section: Discussionmentioning

confidence: 99%

The transient disappearance of cytokeratin in human fetal and adult ovaries

Löffler

Horn

Weber

et al. 2000

Anatomy and Embryology

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Cells from the inner and outer granulosa cell layers of the ovarian follicles differ in function, probably because of their different origins from the surface epithelium and from the rete. This suggestion has not so far been thoroughly investigated in the human ovary. We examined fetal ovaries from the early, middle and late gestational periods, ovaries from fertile women, and preovulatory follicular cells obtained from patients under in vitro fertilization therapy (IVF). Indirect immunohistology and immunocytology were used to detect the presence of cytokeratin (CK)-positive epithelial cells. In fetal ovaries from the early gestational period, prominent rete tubules (sometimes with oocytes) appeared to be fused with the sex cords and primordial follicles. Both showed CK-positively, detected with the pan-CK antibody Lu-5. Cytokeratin 19 was clearly expressed in the fusion area. In the fetal and adult ovaries, CK-positive follicular or granulosa cells were noted in the primordial and primary follicles as well as the preovulatory follicles. Cytokeratin was not detected in the granulosa cells of growing follicles, CK-positive and -negative luteal cells were identified in the developing corpus luteum. We conclude for the human ovary: (1) the heterogeneous morphology of granulosa cells may be explained by their twofold origin from the surface epithelium and the rete, (2) the rete tubules appear to be involved in folliculogenesis, (3) the transient absence of CK expression in growing follicles compared to resting and mature follicles or to the developing corpus luteum indicates a particular role of CK-positive cells at the periovulatory period.

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Section: Discussionmentioning

confidence: 99%

The transient disappearance of cytokeratin in human fetal and adult ovaries

Löffler

Horn

Weber

et al. 2000

Anatomy and Embryology

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“…The presence of CK-positive granulosa cells in preovulatory follicles has not been investigated. The occurrence of CK-positive granulosa cells is species-specific, being found in humans but not in pigs and rats (Czernobilsky et al 1985;Gall et al 1992). Cow ovaries have not been examined until now and little information is available on the appearance of CK-positive cells in corpora lutea.…”

Section: Introductionmentioning

confidence: 96%

Cytokeratin expression in bovine corpora lutea

Ricken

Spanel‐Borowski²,

Saxer³

et al. 1995

Histochem Cell Biol

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Cytokeratin (CK)-positive cells were obtained from bovine corpora lutea. When cultured, these cells behave like CK-positive endothelial cells obtained from bovine large blood vessels. The origin of CK-positive cells has now been studied in 45 bovine corpora lutea of different estrous cycle stages. Additionally, 7 corpora lutea of pregnant cows were examined. The tissues were grouped into early stage (days 2 to 4), secretory stage (days 5 to 17) and late stage (days 18 to 21) according to gross morphology, wet weight and total progesterone content. One portion of a corpus luteum was used for immunohistochemistry, and another for Western blot analysis. Twenty-six of the 45 corpora lutea showed CK expression, as confirmed by immunostaining and Western blotting. Cytokeratin expression was found in all corpora lutea from the early stage, in 14 of 26 corpora lutea from the secretory stage, and 3 of 10 from the late stage. Early stage corpora lutea displayed "zonation" such that a high number of CK-positive luteal cells occurred in the region of the previous granulosa layer and a very low number in the previous thecal layer. Secretory CK-positive corpora lutea showed uniformly distributed, predominantly large luteal cells. In secretory corpora lutea of group A, CK-positive cells and a distinct microvascular tree were seen, the latter visualized by factor VIII-related antigen immunolabelling of endothelial cells. Group B showed none or very few CK-positive cells. Corpora lutea of pregnant cows behaved like corpora lutea of group B. Roughly 1% of CK-positive cells closely associated with the capillary wall were sometimes reminiscent of endothelial cell sprouts.

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“…These so-called "stress filaments" also participate in the maintenance of cell contact between the oocyte and cumulus cells, orchestrate distribution of organelles throughout the cytoplasm of the oocyte, and possibly control resumption of its meiotic division (Gall et al, 1992), in part by influencing cumulus expansion (Šutovský et al, 1995). Most recently, we have identified intermediate filaments, particularly cytokeratin 8/18 filaments, as a possible intrinsic influence of granulosa cell apoptosis during folliculogenesis (Townson et al, 2010).…”

Section: Influence Of the Cytoskeleton On Granulosa Cell/oocyte Viabimentioning

confidence: 99%