Co-expression of glial fabrillary acidic protein and vimentin in reactive astrocytes following brain injury in rats

Calvo, J.; Carbonell, A.L.; Boya, J

doi:10.1016/0006-8993(91)91720-l

Cited by 91 publications

(61 citation statements)

References 16 publications

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“…The cellular levels of all of the above-mentioned enzymes, which very likely play key roles in active transport of ions in their respective cells, were found to be affected by the gentamicin treatment. Furthermore, the intermediate filament vimentin has been shown to be involved in response to tissue damage (Oesterle et al 1990;Calvo et al 1991;Gallanti et al 1992;SundarRaj et al 1992;Usami et al 1993) and has been reported to be present within the cochlea (Anniko et al 1987;Schulte and Adams 1989b;Shi et al 1993). Staining for vimentin was found to be changed in some cells in response to gentamicin treatment.…”

Section: Cytochemical Effects Of Gentamicin Treatmentmentioning

confidence: 99%

“…Calcium-binding proteins and the IP 3 R very likely that is essential for cochlear function (Kikuchi et al 1995). The intermediate filament vimentin has been shown to be involved in response to tissue damage (Oesterle et al 1990;Berggren et al 1990;Calvo et al 1991;Gallanti et al 1992;SundarRaj et al 1992;Usami et al 1993) and has been reported to be present within the cochlea (e.g., Anniko et al 1987;Schulte and Adams 1989b;Shi et al 1993). Staining for neurofilaments was used to judge nerve fiber density.…”

Section: Introductionmentioning

confidence: 99%

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Changes in Cytochemistry of Sensory and Nonsensory Cells in Gentamicin-Treated Cochleas

Imamura

Adams

2003

JARO - Journal of the Association for Research in Otolaryngolog

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Effects of a single local dose of gentamicin upon sensory and nonsensory cells throughout the cochlea were assessed by changes in immunostaining patterns for a broad array of functionally important proteins. Cytochemical changes in hair cells, spiral ganglion cells, and cells of the stria vascularis, spiral ligament, and spiral limbus were found beginning 4 days post administration. The extent of changes in immunostaining varied with survival time and with cell type and was not always commensurate with the degree to which individual cell types accumulated gentamicin. Outer hair cells, types I and II fibrocytes of the spiral ligament, and fibrocytes in the spiral limbus showed marked decreases in immunostaining for a number of constituents. In contrast, inner hair cells, type III fibrocytes and root cells of the spiral ligament, cells of the stria vascularis, and interdental cells in the spiral limbus showed less dramatic decreases, and in some cases they showed increases in immunostaining. Results indicate that, in addition to damaging sensory cells, local application of gentamicin results in widespread and disparate disruptions of a variety of cochlear cell types. Only in the case of ganglion cells was it apparent that the changes in nonsensory cells were secondary to loss or damage of hair cells. These results indicate that malfunction of the ear following gentamicin treatment is widespread and far more complex than simple loss of sensory elements. The results have implications for efforts directed toward detecting, preventing, and treating toxic effects of aminoglycosides upon the inner ear.

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Section: Cytochemical Effects Of Gentamicin Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Changes in Cytochemistry of Sensory and Nonsensory Cells in Gentamicin-Treated Cochleas

Imamura

Adams

2003

JARO - Journal of the Association for Research in Otolaryngolog

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“…Vimentin is another cytoskeletal component, chiefly of immature, undifferentiated glia, which is coexpressed with GFAP in reactive astrocytes following damage to nervous tissue (Calvo et al, 1991). Treatment with dBcAMP induces activation-associated increases in the cytoskeletal components along with many other structural and metabolic constituents of astrocytes (Eddleston and Mucke, 1993) and, in accord with this, greater activation by the βAP fragments when measured by NR uptake, GFAP, vimentin, and S-100 expression.…”

Section: Discussionmentioning

confidence: 91%

Responses of Cultured Astrocytes, C6 Glioma and 1321NI Astrocytoma Cells to Amyloid β-Peptide Fragments

Pentreath

Mead

2004

Nonlinearity in Biology, Toxicology, Medicine

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✷ The effect of amyloid β-peptide (βAP), which can have both neurotrophic or neurotoxic effects on neurons and has been implicated in the pathogenesis of Alzheimer's disease (AD), was studied on astrocytes using primary cultures and astrocyte cell lines (rat C6 glioma, human 1321NI astrocytoma cells). The cultures were exposed to 0.0005-50 µg/ml) βAP fragments 1-40, 25-35, 31-35, or 40-41 (control)

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“…Considering expression pattern of GFAP in retina, it is likely that Muller cells may accumulate GFAP (Calvo et al, 1991;Erickson et al, 1992;Eng and Ghirnikar, 1994). These suggest that injury to retina was secondary to O-glycosylation by glucosamine.…”

Section: Discussionmentioning

confidence: 99%

Dysfunction of Retinal Cell and Optic Nerve by Continuous Cerebroventricular Infusion of Glucosamine

Jang¹,

Han

Jun

et al. 2009

Biomolecules and Therapeutics

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-We have investigated the effect of glucosamine on the retinal cells after continuous infusion into cerebroventricle by using osmotic minipump to avoid peripheral effect. Continuous intracerebroventricular (i.c.v) infusion of glucosamine with the rate of 0.1 μmol/10 μl/hr for 7 days resulted in morphological changes of the optic nerve in electron microscopic level as well as morphological changes of the retina in light microscopic level. Retinal sections were immunostained for the detection of morphological changes of astrocytes. GFAP immunoreactivity appeared not only in the Muller cells but also many of the radial processes of Muller cells. The optic nerve showed deformed axon and slight lamellar separation of myelin sheath after continuous infusion of glucosamine in observing with electron microscope. Interestingly, vacuoles were observed in deformed axons and retinal layers were folded and detached. These results suggested that glucosamine plays a role in induction of morphological dysfunction in retina and optic nerves.

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Co-expression of glial fabrillary acidic protein and vimentin in reactive astrocytes following brain injury in rats

Cited by 91 publications

References 16 publications

Changes in Cytochemistry of Sensory and Nonsensory Cells in Gentamicin-Treated Cochleas

Changes in Cytochemistry of Sensory and Nonsensory Cells in Gentamicin-Treated Cochleas

Responses of Cultured Astrocytes, C6 Glioma and 1321NI Astrocytoma Cells to Amyloid β-Peptide Fragments

Dysfunction of Retinal Cell and Optic Nerve by Continuous Cerebroventricular Infusion of Glucosamine

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