Co‐expression of N‐cadherin and <b>α</b>‐fetoprotein in stomach cancer

Yanagimoto, Kunio; Sato, Yuichi; Shimoyama, Yutaka; Tsuchiya, Benio; Kuwao, Sadahito; Kameya, Toru

doi:10.1046/j.1440-1827.2001.01248.x

Cited by 14 publications

(11 citation statements)

References 33 publications

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“…Interestingly, it has been previously reported that N-cadherin is expressed by a-foetoprotein-positive gastric hepatoid adenocarcinomas. 31 Another diagnostic issue is to discriminate primary intrahepatic cholangiocarcinomas from hepatocellular carcinomas. As the hepatocytes also express N-cadherin at their plasma membrane, this marker cannot 'per se' distinguish hepatocellular carcinomas from intrahepatic cholangiocarcinomas.…”

Section: Discussionmentioning

confidence: 99%

N-cadherin serves as diagnostic biomarker in intrahepatic and perihilar cholangiocarcinomas

et al. 2009

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As a definite immunoprofile of this tumor is missing, the histopathologic diagnosis of intrahepatic cholangiocarcinoma is difficult. The aim of this study was to explore E-and N-cadherin expressions in intrahepatic bile duct tumors, and to determine their potential interest in differential diagnosis. Normal liver tissue, 5 cirrhosis with ductular reaction, 5 focal nodular hyperplasia, 5 bile duct hamartomas, 5 bile duct adenomas, and 45 intrahepatic cholangiocarcinomas from Causasian patients were studied. Tissue-microarrays including 20 esophageal, 86 gastric, 8 small bowel, 64 colonic, 18 pancreatic, 6 gallbladder, and 7 extrahepatic biliary tract adenocarcinomas, 22 hepatocellular carcinomas, and normal tissues were constructed. Immunohistochemistry was performed using E-cadherin, N-cadherin, NCAM, Hep Par1, and cytokeratins 7, 19 and 20. Immunoblot analysis of frozen liver tissues was performed to control the specificity of E-and N-cadherin antibodies used. In normal liver, epithelial cells of intrahepatic bile ducts, whatever their caliber, as well as hepatocytes, coexpressed E-and N-cadherins at their plasma membranes. In cirrhosis, ductular reactions completely expressed E-and N-cadherins. All the benign lesions and 30 of the 45 intrahepatic cholangiocarcinomas (23/29 peripheral and 7/16 hilar) also expressed N-cadherin. E-cadherin was detected in all the lesions. The expression of N-cadherin at the plasma membrane of tumor cells was significantly more frequent in peripheral than in hilar intrahepatic cholangiocarcinomas (P ¼ 0.003). Among noncholangiocarcinomas, only 1% gastric and 66% gallbladder adenocarcinomas and all the hepatocellular carcinomas expressed N-cadherin at the membrane of tumor cells. Finally, for the diagnosis of intrahepatic cholangiocarcinomas, the specificity value of membranous expression of N-cadherin was 88%, whereas that of the combination cytokeratin 7/membranous N-cadherin was 98%. In the gastrointestinal and liver tract, membranous N-cadherin is restricted to the hepatocytes and intrahepatic biliary cells. In combination with cytokeratin 7 and Hep Par1, N-cadherin is a reliable tool for the histopathological diagnosis of primary hepatic tumors.

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Section: Discussionmentioning

confidence: 99%

N-cadherin serves as diagnostic biomarker in intrahepatic and perihilar cholangiocarcinomas

et al. 2009

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“…In prostate cancers, de novo expression of mesenchymal cadherins, N‐cadherin, and cadherin‐11, is observed in the poorly differentiated areas where E‐cadherin expression is lost or aberrant, suggesting that a ‘cadherin switch’ from epithelial to mesenchymal cadherins is related to the transition from a benign to invasive phenotype 18 . However, N‐cadherin is detected in AFP‐producing gastric adenocarcinomas with normal E‐cadherin expression, 19 and directly promotes cell motility and invasion in breast cancer cells 11 . Several molecular mechanisms have been proposed, which include N‐cadherin‐mediated dynamic adhesion between tumor cells, interactions with the surrounding stromal cells, 18,20 activation of fibroblast growth factor receptor, 11 and prevention of cell apoptosis by inhibiting the increase in intracellular free calcium 21 .…”

Section: Discussionmentioning

confidence: 99%

Neural cadherin overexpression is a predictive marker for early postoperative recurrence in hepatocellular carcinoma patients

Seo

Lee²,

Kim³

et al. 2008

J of Gastro and Hepatol

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“…N-cadherin was found back in metastasising melanomas (Matsuyoshi et al, 1997;Sanders et al, 1999). In gastric carcinoma N-cadherin was found in all α-foetoprotein producing tumours (Yanagimoto et al, 2001) and a correlation was found between the expression of twist and N-cadherin expression (Rosivatz et al, 2002). During early development N-cadherin is found in some basal granulated epithelial cells of the stomach, duodenum and jejunum (Gaidar et al, 1998).…”

Section: N-cadherin and Cancermentioning

confidence: 99%

N-cadherin in the spotlight of cell-cell adhesion, differentiation, embryogenesis, invasion and signalling

Derycke

Bracke²

2004

Int. J. Dev. Biol.

361

284

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Cell migration is a process which is essential during embryonic development, throughout adult life and in some pathological conditions. Cadherins, and more specifically the neural cell adhesion molecule N-cadherin, play an important role in migration. In embryogenesis, N-cadherin is the key molecule during gastrulation and neural crest development. N-cadherin mediated contacts activate several pathways like Rho GTPases and function in tyrosine kinase signalling (for example via the fibroblast growth factor receptor). In cancer, cadherins control the balance between suppression and promotion of invasion. E-cadherin functions as an invasion suppressor and is downregulated in most carcinomas, while N-cadherin, as an invasion promoter, is frequently upregulated. Expression of N-cadherin in epithelial cells induces changes in morphology to a fibroblastic phenotype, rendering the cells more motile and invasive. However in some cancers, like osteosarcoma, N-cadherin may behave as a tumour suppressor. N-cadherin can have multiple functions: promoting adhesion or induction of migration dependent on the cellular context. KEY WORDS: N-cadherin, cancer, embryogenesis, invasion, signallingInt. J. Dev. Biol. 48: 463-476 (2004) Migration and invasionCell migration is a process that is essential during embryonic development and throughout further life. In the adult, cell migration is crucial for homeostatic processes, such as effective immune responses and repair of injured tissues. To migrate, the individual cell body must modify its shape to interact with the surrounding tissue structures. The extracellular matrix (ECM) forms a substrate, as well as a barrier for the advancing cell body. Cell migration through tissues results from a continuous cycle of interdependent steps. In general, there are five steps involved in cell migration in the ECM. First comes the protrusion of the leading edge, where growing actin filaments connect to adapter proteins and push the cell membrane in an outward direction. In a second step cell-matrix interactions and focal contacts are formed. After that, surface proteases such as matrix metalloproteinases (MMP) are recruited and focal proteolysis takes place. Then the cell contracts by actomyosin activation, and finally the tail of the cell is detached from its substrate (Friedl and Wolf, 2003).Border cells of the Drosophila melanogaster ovary are nowadays used as a model for migration. There are three recently discovered signalling pathways that control distinct aspects of migration: a global steroid-hormone signal defines the timing of migration, a highly localised cytokine signal that activates the Janus kinase-signal transducer and activator of transcription is both necessary and sufficient to induce migration, and finally, a growth factor that is analogous to platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) contributes to guiding the cells to their destination (Montell, 2003).In embryonic morphogenesis two types of collective cell movement can ...

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Co‐expression of N‐cadherin and α‐fetoprotein in stomach cancer

Cited by 14 publications

References 33 publications

N-cadherin serves as diagnostic biomarker in intrahepatic and perihilar cholangiocarcinomas

N-cadherin serves as diagnostic biomarker in intrahepatic and perihilar cholangiocarcinomas

Neural cadherin overexpression is a predictive marker for early postoperative recurrence in hepatocellular carcinoma patients

N-cadherin in the spotlight of cell-cell adhesion, differentiation, embryogenesis, invasion and signalling

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