Co-expression of vacuolar Na+/H+ antiporter and H+-pyrophosphatase with an IRES-mediated dicistronic vector improves salinity tolerance and enhances potassium biofortification of tomato

Gouiaa, Sandra; Khoudi, Habib

doi:10.1016/j.phytochem.2015.05.016

Cited by 34 publications

(18 citation statements)

References 58 publications

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“…A total of 0.5 g of fresh leaves was extracted for the measurements of chlorophyll, proline, solute sugar, and malondialdehyde (MDA) [19]. Measurements of Na and K in the shoots were performed by atomic absorption [45]. The relative water contents were determined as previously described [10].…”

Section: Methodsmentioning

confidence: 99%

Ectopic Expression of a Thellungiella salsuginea Aquaporin Gene, TsPIP1;1, Increased the Salt Tolerance of Rice

Wei

Qiang

Han

et al. 2018

IJMS

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Aquaporins play important regulatory roles in the transport of water and small molecules in plants. In this study, a Thellungiella salsuginea TsPIP1;1 aquaporin was transformed into Kitaake rice, and three transgenic lines were evaluated by profiling the changes of the physiological metabolism, osmotic potential, and differentially expressed genes under salt stress. The TsPIP1;1 protein contains six transmembrane domains and is localized in the cytoplasm membrane. Overexpression of the TsPIP1;1 gene not only increased the accumulation of prolines, soluble sugars and chlorophyll, but also lowered the osmotic potential and malondialdehyde content in rice under salt stress, and alleviated the amount of salt damage done to rice organs by regulating the distribution of Na/K ions, thereby promoting photosynthetic rates. Transcriptome sequencing confirmed that the differentially expressed genes that are up-regulated in rice positively respond to salt stimulus, the photosynthetic metabolic process, and the accumulation profiles of small molecules and Na/K ions. The co-expressed Rubisco and LHCA4 genes in rice were remarkably up-regulated under salt stress. This data suggests that overexpression of the TsPIP1;1 gene is involved in the regulation of water transport, the accumulation of Na/K ions, and the translocation of photosynthetic metabolites, thus conferring enhanced salt tolerance to rice.

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Section: Methodsmentioning

confidence: 99%

Ectopic Expression of a Thellungiella salsuginea Aquaporin Gene, TsPIP1;1, Increased the Salt Tolerance of Rice

Wei

Qiang

Han

et al. 2018

IJMS

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“…Nevertheless, engineering of vacuolar cation/H + antiporter coupled with its H + -translocating pyrophosphatase (H + -PPase) often leads to increased salt stress tolerance. Therefore, overexpression of the wheat TNHX1 and TVP1 improves salt stress tolerance in Arabidopsis (Brini et al, 2007a), tobacco (Gouiaa et al, 2012), and tomato (Gouiaa and Khoudi, 2015). Alone the Arabidopsis H+-PPase (AtAVP1) was also demonstrated to increase stress tolerance in a crop plant.…”

Section: Strategies To Improve Salt Tolerance In Cropsmentioning

confidence: 99%

New Insights on Plant Salt Tolerance Mechanisms and Their Potential Use for Breeding

et al. 2016

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Soil salinization is a major threat to agriculture in arid and semi-arid regions, where water scarcity and inadequate drainage of irrigated lands severely reduce crop yield. Salt accumulation inhibits plant growth and reduces the ability to uptake water and nutrients, leading to osmotic or water-deficit stress. Salt is also causing injury of the young photosynthetic leaves and acceleration of their senescence, as the Na+ cation is toxic when accumulating in cell cytosol resulting in ionic imbalance and toxicity of transpiring leaves. To cope with salt stress, plants have evolved mainly two types of tolerance mechanisms based on either limiting the entry of salt by the roots, or controlling its concentration and distribution. Understanding the overall control of Na+ accumulation and functional studies of genes involved in transport processes, will provide a new opportunity to improve the salinity tolerance of plants relevant to food security in arid regions. A better understanding of these tolerance mechanisms can be used to breed crops with improved yield performance under salinity stress. Moreover, associations of cultures with nitrogen-fixing bacteria and arbuscular mycorrhizal fungi could serve as an alternative and sustainable strategy to increase crop yields in salt-affected fields.

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“…Very likely, this vacuolar route of CmPP working in yeast might be applicable to CmPP transgenic tobacco with increased salt tolerance. Moreover, probably due to an attribute of Na þ -regulated Na þ ,H þ -PPase, the tobacco vacuole-targeted CmPP might be unable to exert its H þ -pumping activity that indirectly drive Na þ flux into vacuoles and alleviate cytosolic Na þ toxicity under salt stresses, as did the canonical H þ -PPases reported in many previous studies (Bao et al, 2009;Gouiaa & Khoudi, 2015;Liu et al, 2011;Pasapula et al, 2011;Schilling et al, 2014).…”

Section: Discussionmentioning

confidence: 98%

“…Particularly, numerous H þ -PPase-overexpressing transgenic plants demonstrate improved tolerance to salt, drought, chilling, nutrition (e.g. nitrogen, phosphorus, potassium) deprivation, and heavy metal environment (Bao et al, 2009;Gaxiola et al, 2012;Gouiaa & Khoudi, 2015;Khoudi et al, 2012;Liu et al, 2011, Pasapula et al, 2011Paez-Valencia et al, 2013;Schilling et al, 2014Schilling et al, , 2017Wei et al, 2011;Yang et al, 2007;Zhang et al, 2011). Many of those engineered plants even outperform controls with greater yields or biomass under normal and stress conditions.…”

Section: Introductionmentioning

confidence: 99%

Membrane-bound pyrophosphatase of human gut microbe Clostridium methylpentosum confers improved salt tolerance in Escherichia coli, Saccharomyces cerevisiae and tobacco

Yang

Liu

Yuan

et al. 2016

Molecular Membrane Biology

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Membrane-bound pyrophosphatases (PPases) are involved in the adaption of organisms to stress conditions, which was substantiated by numerous plant transgenic studies with H-PPase yet devoid of any correlated evidences for other two subfamilies, Na-PPase and Na,H-PPase. Herein, we demonstrate the gene cloning and functional evaluation of the membrane-bound PPase (CmPP) of the human gut microbe Clostridium methylpentosum. The CmPP gene encodes a single polypeptide of 699 amino acids that was predicted as a multi-spanning membrane and K-dependent Na,H-PPase. Heterologous expression of CmPP could significantly enhance the salt tolerance of both Escherichia coli and Saccharomyces cerevisiae, and this effect in yeast could be fortified by N-terminal addition of a vacuole-targeting signal peptide from the H-PPase of Trypanosoma cruzi. Furthermore, introduction of CmPP could remarkably improve the salt tolerance of tobacco, implying its potential use in constructing salt-resistant transgenic crops. Consequently, the possible mechanisms of CmPP to underlie salt tolerance are discussed.

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Co-expression of vacuolar Na+/H+ antiporter and H+-pyrophosphatase with an IRES-mediated dicistronic vector improves salinity tolerance and enhances potassium biofortification of tomato

Cited by 34 publications

References 58 publications

Ectopic Expression of a Thellungiella salsuginea Aquaporin Gene, TsPIP1;1, Increased the Salt Tolerance of Rice

Ectopic Expression of a Thellungiella salsuginea Aquaporin Gene, TsPIP1;1, Increased the Salt Tolerance of Rice

New Insights on Plant Salt Tolerance Mechanisms and Their Potential Use for Breeding

Membrane-bound pyrophosphatase of human gut microbe Clostridium methylpentosum confers improved salt tolerance in Escherichia coli, Saccharomyces cerevisiae and tobacco

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