Co-expression patterns of cocaine- and amphetamine-regulated transcript (CART) with neuropeptides in dorsal root ganglia of the pig

Zacharko-Siembida, Anna; Kulik, Paweł; Szalak, Radosław; Lalak, Roman; Arciszewski, Marcin B.

doi:10.1016/j.acthis.2013.09.006

Cited by 10 publications

(14 citation statements)

References 53 publications

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“…46% of neuronal profiles showed the presence of CART (Richardson et al 2006). Recently, a minor population of CART-IR neurons in intramural ganglia of the porcine urinary bladder trigone has been described and characterized (Zacharko-Siembida and Arciszewski 2014). Taken together, these findings suggest that CART-IR parasympathetic neurons may play certain, however till now largely unknown, function(s).…”

Section: Discussionmentioning

confidence: 53%

“…Interestingly, highly selective expression of CART has been also described in peripheral ganglia (including enteric) and in certain organs of many animal species including the rat , Fenwick et al 2006, Richardson et al 2006, Ivanusic et al 2012, mouse (Gautron et al 2012), sheep (Arciszewski et al 2008), cow (Janiuk et al 2013) and pig (Wierup et al 2006(Wierup et al , 2007. In previous reports we and other researchers also described the presence of CART and the co-localization patterns with neuroactive peptides in porcine peripheral ganglia like enteric ganglia of the stomach (Zacharko-Siembida and Arciszewski 2014a), small and large intestines (Gonkowski et al 2009, Burliński et al 2014, dorsal root ganglia (Zacharko-Siembida et al 2014) and ganglia of the urinary bladder (Zacharko-Siembida and Arciszewski 2014b). Considering the wide distribution of CART peptides in the porcine peripheral nervous system we speculate it is likely that in this animal species CART may be expressed in other (so far not investigated) ganglionic neurons.…”

Section: Introductionmentioning

confidence: 92%

“…The presence of SP-IR varicose basket-like formations around CART-IR pelvic ganglia neurons suggests the direct neuromodulatory role of SP in passing signals through CART-ergic neurons. Noteworthy, previously the co-localization of CART and SP was found only in neurons of the central nuclei (Hubert and Kuhar 2005), and in small subsets of neurons in porcine dorsal root ganglia (Zacharko-Siembida et al 2014) or enteric ganglia (Gonkowski et al 2009). In male rodents, which are known not to express SP in MPG neurons, pericellular SP-IR nerve fibres are provided to the PP both via the hypogastric and pelvic nerves (Dhami and Mitchell 1991).…”

Section: Discussionmentioning

confidence: 99%

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Distribution and immunohistochemical characteristics of cocaine- and amphetamineregulated transcript-positive nerve elements in the pelvic ganglia of the female pig

Zacharko-Siembida¹,

Matysek²,

Szalak³

et al. 2017

Polish Journal of Veterinary Sciences

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Cocaine-and amphetamine-regulated transcript (CART) peptides are widely expressed not only in the brain but also in numerous endocrine/neuroendocrine cells as well as in neurons of the peripheral nervous system. The present study investigated the distribution patterns of CART-like immunoreactivity in the pelvic plexus (PP) of the female pig. The co-expression of CART with principal neurotransmitter markers: choline acetyltransferase (ChAT), tyrosine hydroxylase (TH), serotonin (5-HT) or biologically active neuropeptides: pituitary adenylate cyclase-activating polypeptide (PACAP), substance P (SP), calbindin was analyzed using double immunohistochemical stainings. Amongst neurons immunopositive to Hu C/D panneuronal marker as many as 4.1 ± 1.2% in right and 4.4 ± 1.6% in left pelvic ganglia were found to express CART. The vast majority of CART-IR ganglionic neurons were predominantly small in size and were evenly scattered throughout particular ganglia. Immunoreactivity to CART was also detected in numerous nerve terminals (which frequently formed pericellular formations around CART-negative perikarya) as well as in numerous nerve fibres within nerve branches interconnecting the unilateral pelvic ganglia. Immunohistochemistry revealed that virtually all CART-IR neurons were cholinergic in nature and CART-IR basket-like formations frequently encircled TH-positive/CART-negative perikarya. None of CART-IR ganglionic neurons showed immunoreactivity to SP, PACAP, 5-HT or calbindin. CART-IR nerve fibres ran in a close vicinity to serotonin-containing cells or faintly labelled SP-expressing neurons. On the other hand, PACAP-IR, SP-IR (but not 5-HT-positive) nerve terminals were found to run in close proximity to CART-IR neurons. Our results indicate that: 1) CART present in PP may influence the activity of pelvic ganglionic neurons/SIF cells, 2) PP should be considered as a potential source of CART-like supply to pelvic viscera and 3) functional interactions between CART and SP or PACAP are possible at the periphery.

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Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Distribution and immunohistochemical characteristics of cocaine- and amphetamineregulated transcript-positive nerve elements in the pelvic ganglia of the female pig

Zacharko-Siembida¹,

Matysek²,

Szalak³

et al. 2017

Polish Journal of Veterinary Sciences

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“…Nevertheless, the present data indicate that, at least in the pig, L-ENK + projections to CaMG must originate from another source. It is possible that L-ENK + NF supplying CaMG neurons may represent collaterals of peripheral processes of DRG neurons, because, in the pig, they have been found to stain for L-ENK (Zacharko- Siembida et al 2014). The association of the L-ENK + axons with viscerofugal projections from the gastrointestinal tract (suggested previously by Kaleczyc et al 2003) should also be taken into consideration.…”

Section: Discussionmentioning

confidence: 93%

“…It is possible that L‐ENK + NF supplying CaMG neurons may represent collaterals of peripheral processes of DRG neurons, because, in the pig, they have been found to stain for L‐ENK (Zacharko‐Siembida et al. ). The association of the L‐ENK + axons with viscerofugal projections from the gastrointestinal tract (suggested previously by Kaleczyc et al.…”

Section: Discussionmentioning

confidence: 99%

A study on preganglionic connections and possible viscerofugal projections from urinary bladder intramural ganglia to the caudal mesenteric ganglion in the pig

et al. 2018

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The present study was designed to (1) ascertain the distribution and immunohistochemical characteristics of sympathetic preganglionic neurons supplying the caudal mesenteric ganglion (CaMG) and (2) verify the existence of viscerofugal projections from the urinary bladder trigone intramural ganglia (UBT‐IG) to the CaMG in female pigs (n = 6). Combined retrograde tracing and immunofluorescence methods were used. Injections of the neuronal tracer Fast Blue (FB) into the right CaMG revealed no retrogradely labelled (FB‐positive; FB+) nerve cells in the intramural ganglia; however, many FB+ neurons were found in the spinal cord sympathetic nuclei. Double‐labelling immunohistochemistry revealed that nearly all (99.4 ± 0.6%) retrogradely labelled neurons were cholinergic (choline acetyltransferase‐positive; ChAT+) in nature. Many FB+/ChAT+ perikarya stained positive for vesicular acetylcholine transporter (63.11 ± 5.34%), neuronal nitric oxide synthase (53.48 ± 9.62%) or cocaine‐ and amphetamine‐regulated transcript peptide (41.13 ± 4.77%). A small number of the retrogradely labelled cells revealed immunoreactivity for calcitonin gene‐related peptide (7.60 ± 1.34%) or pituitary adenylate cyclase‐activating polypeptide (4.57 ± 1.43%). The present study provides the first detailed information on the arrangement and chemical features of preganglionic neurons projecting to the porcine CaMG and, importantly, strong evidence suggesting the absence of viscerofugal projections from the UBT‐IG.

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Cocaine- and amphetamine-regulated transcript (CART) peptide in the enteric nervous system of the porcine esophagus

Makowska

Rytel

Lech³

et al. 2018

Comptes Rendus. Biologies

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Cocaine- and amphetamine-regulated transcript peptide (CART) is widely distributed within the central and peripheral nervous system. In the brain, CART is considered as the main anorectic peptide involved in the regulation of food intake. Contrary to the central nervous system, a lot of aspects connected with the distribution and functions of CART within the enteric nervous system (ENS) still remain unknown. The aim of the present study was to investigate, for the first time, the population of CART-like immunoreactive (CART-LI) neurons within the porcine esophagus and the denotation of their neurochemical coding. During this experiment, the distribution of CART-LI neurons and the colocalization of CART with other neuronal active substances were examined using standard double- and triple-immunofluorescence techniques in enteric plexuses of cervical, thoracic, and abdominal esophagus fragments. The obtained results showed that CART is present in a relatively high percentage of esophageal neurons (values fluctuated from 45.2±0.9% in the submucous plexus of the thoracic esophagus to 58.1±5.0% in the myenteric plexus of the same fragment of the esophagus). Moreover, CART colocalized with a wide range of other active neuronal substances, mainly with the vesicular acetylcholine transporter (VAChT, a marker of cholinergic neurons), neuronal isoform of nitric oxide synthase (nNOS, a marker of nitrergic neurons), vasoactive intestinal polypeptide (VIP) and galanin (GAL). The number of CART-positive neuronal cells and their neurochemical coding clearly depended on the fragment of esophagus studied and the type of enteric plexus. The obtained results suggest that CART may play important and multidirectional roles in the neuronal regulation of esophageal functions.

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Co-expression patterns of cocaine- and amphetamine-regulated transcript (CART) with neuropeptides in dorsal root ganglia of the pig

Cited by 10 publications

References 53 publications

Distribution and immunohistochemical characteristics of cocaine- and amphetamineregulated transcript-positive nerve elements in the pelvic ganglia of the female pig

Distribution and immunohistochemical characteristics of cocaine- and amphetamineregulated transcript-positive nerve elements in the pelvic ganglia of the female pig

A study on preganglionic connections and possible viscerofugal projections from urinary bladder intramural ganglia to the caudal mesenteric ganglion in the pig

Cocaine- and amphetamine-regulated transcript (CART) peptide in the enteric nervous system of the porcine esophagus

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