Co(III) complex with (E)-2-(2-(pyridine-2-ylmethylene)hydrazinyl)-4-(4-tolyl)-1,3-thiazole: structure and activity against 2-D and 3-D cancer cell models

Abstract

Co(III) complex with a 2-hydrazonylthiazole ligand was synthesized and characterized by single crystal X-ray diffraction. In the inner sphere of the complex two monoionic ligands are coordinated tridentate forming octahedral geometry around Co(III). Activity of the complex was investigated on MCF-7 breast cancer cell line, with cisplatin (CDDP) as a reference compound.Results showed that after 24 h incubation Co(III) complex revealed stronger cytotoxic activity compared to CDDP. Treatment of MCF-7 3-D cell model with the complex at 10 µM concentration achieved complete suppression of spheroid growth in almost the same extent as at 100 µM. In combination treatments on MCF-7 spheroids, the complex acted synergistically with CDDP, while additive interaction type was achieved when the complex was applied together with paclitaxel.

A novel binuclear hydrazone-based Cd(II) complex is a strong pro-apoptotic inducer with significant activity against 2D and 3D pancreatic cancer stem cells

“…For CDDP, examined on MCF-7 3D model previously [150], the current study confirms its ability to reduce initial size of treated spheroids at concentrations of 10 μM (0.85 ± 0.04-fold) and 100 μM (0.81 ± 0.05-fold). Nevertheless, results on AsPC-1 spheroids disclose an interesting similarity in pattern of 1 and CDDP activities (Figs.…”

Cobalt Complex with Thiazole-Based Ligand as New Pseudomonas aeruginosa Quorum Quencher, Biofilm Inhibitor and Virulence Attenuator

“…1 H and 13 C NMR spectra were performed on Bruker Avance 500, equipped with a broad-band direct probe. All spectra were measured at 298 K. Co( HL ) 2 was prepared by reaction of HL and Co(BF 4 ) 2 ·6H 2 O in MeOH as emerald colored single crystals as described previously [ 17 ]. IR and NMR spectroscopy data, as well as molar conductivity measurements and results of elemental analysis for Co( HL ) 2 are in good agreement with the data previously published [ 17 ].…”

“…All spectra were measured at 298 K. Co( HL ) 2 was prepared by reaction of HL and Co(BF 4 ) 2 ·6H 2 O in MeOH as emerald colored single crystals as described previously [ 17 ]. IR and NMR spectroscopy data, as well as molar conductivity measurements and results of elemental analysis for Co( HL ) 2 are in good agreement with the data previously published [ 17 ]. The UV/Vis spectra during a 24 h period were recorded with a GBC Scientific Cintra 6 UV/Vis spectrophotometer (250–800 nm) with sample dissolved in DMSO and diluted with water such that the final DMSO content was 6% (vv −1 ).…”

“…Ligand structures were based on the X-ray crystal structure [ 17 ]. The protein crystal structure of the transcriptional activator protein LasR-3-oxo-C12-HSL complex was downloaded from the Protein Databank (1ix3) [Protein Data Bank.…”

“…Due to poor solubility in culture medium, the activity of HL could not be tested, so its cobalt(III) complex ([Co( HL ) 2 ]BF 4 , Scheme 1 ) was prepared. Our results revealed that Co( HL ) 2 showed stronger activity on human mammary adenocarcinoma (MCF-7) cancer cells than cisplatin [ 17 ]. Additionally, the antibacterial activity of Co( HL ) 2 was tested by the disc diffusion method on several Gram-positive ( Staphylococcus aureus , Clostridium sporogenes , Bacillus subtilis, and Kocuria rhizophila ) and Gram-negative ( Proteus hauseri , P. aeruginosa , Escherichia coli , and Salmonella enterica ) strains.…”