Co-immobilization of ACH11 antithrombotic peptide and CAG cell-adhesive peptide onto vascular grafts for improved hemocompatibility and endothelialization

Zhao, Jing; Bai, Lingchuang; Ren, Xiang‐Kui; Guo, Jintang; Xia, Shihai; Zhang, Wencheng; Feng, Yakai

doi:10.1016/j.actbio.2019.07.057

Cited by 50 publications

(34 citation statements)

References 84 publications

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“…The bands of glycosidic linkage and C–O–C of agarose were observed at 1151 and 1040 cm −1 [ 31 ]. PDA exhibited a characteristic band at 1538 cm −1 , which represents the C–N bending vibration of the indolequinone groups [ 32 , 33 ]. Considering the spectra of agarose derived hydrogels (AHG and APG3), it was evident that most adsorption bands were retained as compared with pure agarose.…”

Section: Resultsmentioning

confidence: 99%

Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering

Zhang

Zeng

et al. 2021

Bioactive Materials

165

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Polysaccharide hydrogels are widely used in tissue engineering because of their superior biocompatibility and low immunogenicity. However, many of these hydrogels are unrealistic for practical applications as the cost of raw materials is high, and the fabrication steps are tedious. This study focuses on the facile fabrication and optimization of agarose-polydopamine hydrogel (APG) scaffolds for skin wound healing. The first study objective was to evaluate the effects of polydopamine (PDA) on the mechanical properties, water holding capacity and cell adhesiveness of APG. We observed that APG showed decreased rigidity and increased water content with the addition of PDA. Most importantly, decreased rigidity translated into significant increase in cell adhesiveness. Next, the slow biodegradability and high biocompatibility of APG with the highest PDA content (APG3) was confirmed. In addition, APG3 promoted full-thickness skin defect healing by accelerating collagen deposition and promoting angiogenesis. Altogether, we have developed a straightforward and efficient strategy to construct functional APG scaffold for skin tissue engineering, which has translation potentials in clinical practice.

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Section: Resultsmentioning

confidence: 99%

Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering

Zhang

Zeng

et al. 2021

Bioactive Materials

165

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“…Electrospinning is a widely employed technique to obtain biodegradable grafts (Park et al, 2019), which has been in some cases combined with surface functionalization to promote endothelialization and improve hemocompatibility (Zhao et al, 2019). However, electrospun scaffolds typically suffer from poor cellular infiltration because of the dense fibrous network (Zhong et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Small Caliber Compliant Vascular Grafts Based on Elastin-Like Recombinamers for in situ Tissue Engineering

Fernández‐Colino

Wolf

Rütten

et al. 2019

Front. Bioeng. Biotechnol.

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Vascular disease is a leading cause of death worldwide, but surgical options are restricted by the limited availability of autologous vessels, and the suboptimal performance of prosthetic vascular grafts. This is especially evident for coronary artery by-pass grafts, whose small caliber is associated with a high occlusion propensity. Despite the potential of tissue-engineered grafts, compliance mismatch, dilatation, thrombus formation, and the lack of functional elastin are still major limitations leading to graft failure. This calls for advanced materials and fabrication schemes to achieve improved control on the grafts' properties and performance. Here, bioinspired materials and technical textile components are combined to create biohybrid cell-free implants for endogenous tissue regeneration. Clickable elastin-like recombinamers are processed to form an open macroporous 3D architecture to favor cell ingrowth, while being endowed with the non-thrombogenicity and the elastic behavior of the native elastin. The textile components (i.e., warp-knitted and electrospun meshes) are designed to confer suture retention, long-term structural stability, burst strength, and compliance. Notably, by controlling the electrospun layer's thickness, the compliance can be modulated over a wide range of values encompassing those of native vessels. The grafts support cell ingrowth, extracellular matrix deposition and endothelium development in vitro. Overall, the fabrication strategy results in promising off-the-shelf hemocompatible vascular implants for in situ tissue engineering by addressing the known limitations of bioartificial vessel substitutes.

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“…DOPA‐pCB was the most effective, with fibrin and gross thrombus formation decreasing by 59% and applicable to multiple surface materials. Other biomolecules such as polyelectrolytes, [ 48 ] ACH 11 antithrombotic peptide, [ 49 ] reactive oxygen species (ROS)‐responsive antithrombogenic drug, [ 41 ] and Arg‐Glu‐Asp‐Val (REDV) peptide, [ 50 ] were also embedded or grafted to vascular grafts for anticoagulation. Dextran sulfate was self‐assembled onto an electrospun chitosan/PCL scaffold.…”

Section: Immunocompatibility Of Engineered Tissue or Organ‐based Implantsmentioning

confidence: 99%

Tissue Engineering for Mimics and Modulations of Immune Functions

Tao

Wang

2021

Adv Healthcare Materials

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In the field of regenerative medicine, advances in tissue engineering have surpassed the reconstruction of individual tissues or organs and begun to work towards engineering systemic factors such as immune objects and functions. The immune system plays a crucial role in protecting and regulating systemic functions in the human body. Engineered immune tissues and organs have shown potential in recovering dysfunctions and aplasia of the immune system and the evasion from immune‐mediated inflammatory responses and rejection elicited by engineered implants from allogeneic or xenogeneic sources are also being pursued to facilitate clinical transplantation of tissue engineered grafts. Here, current progress in tissue engineering to mimic or modulate immune functions is reviewed and elaborated from two perspectives: 1) engineering of immune tissues and organs per se and 2) immune evasion of host immunoinflammatory rejection by tissue‐engineered implants.

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Co-immobilization of ACH11 antithrombotic peptide and CAG cell-adhesive peptide onto vascular grafts for improved hemocompatibility and endothelialization

Cited by 50 publications

References 84 publications

Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering

Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering

Small Caliber Compliant Vascular Grafts Based on Elastin-Like Recombinamers for in situ Tissue Engineering

Tissue Engineering for Mimics and Modulations of Immune Functions

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