Co-immobilization of cholesterol oxidase and horseradish peroxidase in a sol–gel film

Kumar, Arun; Malhotra, Rajesh; Malhotra, Bansi D.; Grover, Shivani

doi:10.1016/s0003-2670(00)00792-3

Cited by 127 publications

(36 citation statements)

References 20 publications

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“…Additionally, the detection limit of the biosensor, based on a signal-to-noise ratio of 3, was 0.2 mM. This value is similar to that reported for a polypyrrole bienzyme electrode (0.2 mM) [29] and smaller than that reported for a ChOx-HRP sol-gel sensor (0.5 mM) [30]. However, it is significantly higher than that reported for many cholesterol biosensors [16,17] (< 10 mM).…”

supporting

confidence: 77%

Carbon Nanotubes‐Based Amperometric Cholesterol Biosensor Fabricated Through Layer‐by‐Layer Technique

et al. 2004

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A carbon nanotubes-based amperometric cholesterol biosensor has been fabricated through layer-by-layer (LBL) deposition of a cationic polyelectrolyte (PDDA, poly(diallyldimethylammonium chloride)) and cholesterol oxidase (ChOx) on multi-walled carbon nanotubes (MWNTs)-modified gold electrode, followed by electrochemical generation of a nonconducting poly(o-phenylenediamine) (PPD) film as the protective coating. Electrochemical impedance measurements have shown that PDDA/ChOx multilayer film could be formed uniformly on MWNTsmodified gold electrode. Due to the strong electrocatalytic properties of MWNTs toward H 2 O 2 and the low permeability of PPD film for electroacitve species, such as ascorbic acid, uric acid and acetaminophen, the biosensor has shown high sensitivity and good anti-interferent ability in the detection of cholesterol. The effect of the pH value of the detection solution on the response of the biosensor was also investigated. A linear range up to 6.0 mM has been observed for the biosensor with a detection limit of 0.2 mM. The apparent Michaelis-Menten constant and the maximum response current density were calculated to be 7.17 mM and 7.32 mA cm À2, respectively.

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confidence: 77%

Carbon Nanotubes‐Based Amperometric Cholesterol Biosensor Fabricated Through Layer‐by‐Layer Technique

et al. 2004

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“…However, the usefulness of immobilized enzyme on electrodes depends on factors such as the immobilization method, the chemical and physical conditions (such as pH, temperature and contaminants), thickness and stability of the membrane used to couple the enzyme. Immobilization of enzyme in several matrices has been used for the fabrication of biosensors for estimation of glucose [3], lactate [4], cholesterol [5] etc. Various physical and chemical deposition techniques have been utilized to prepare thin films including chemical bath deposition, dip coating, Spray Pyrolysis Method and spin coating.…”

Section: Introductionmentioning

confidence: 99%

Influence of silane content on the optical properties of sol gel derived spin coated silica thin films

Satvekar

Phadatare²,

Karande³

et al. 2012

International Journal of Basic and Applied Sciences

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Sol gel technique in combination with spin coating method was utilized for the synthesis of silica thin films. Tetraethyl orthosilicate (TEOS) alkoxide silane precursor was used for one step synthesis of silica sol with 0.1M HCL as a catalyst. Casting solution of silica sol was spin coated on glass substrate. The X-ray diffraction pattern revealed that the sample possesses amorphous silica matrix. Thin films were characterized by Fourier transform infrared spectroscopy (FTIR) and UV-Visible spectroscopy techniques to compare the effect of TEOS concentration. The optical properties of the silica thin films revealed that thin films have 90-95% transmittance in the visible range.

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“…Sol -gel-derived glasses have emerged in recent years as a new class of material well suited to the immobilization of biomolecules such as enzymes and for these reasons have been widely applied to the development of electrochemical biosensors [29 -36]. An enzyme can be immobilized by building the porous gel network around each enzyme macromolecule by encapsulation that does not involve formation of any covalent bond between the support and the enzyme, thus allowing the preservation of enzyme activity [35]. The hydrophilic -hydrophobic nature of the silica gels ensures good compatibility in their application to biosensors [37].…”

Section: Introductionmentioning

confidence: 99%

Development and Application of Oxysilane Sol–Gel Electrochemical Glucose Biosensors Based on Cobalt Hexacyanoferrate Modified Carbon Film Electrodes

et al. 2007

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The evaluation and characterization of a new glucose oxidase (GOx) electrochemical enzyme biosensors on a carbon film electrode support and using two types of sol -gel precursor mixture for enzyme immobilization was carried out in order to be employed as a short-time-use or as a disposable sensor. Carbon film electrodes were modified with films of CoHCF mediator by potential cycling. The sol -gel was prepared using a combination of 3-glycidoxypropyltrimethoxysilane (GOPMOS) with methyltrimethoxysilane (MTMOS) or tetraethoxysilane (TEOS). The best biosensor sensitivity (ca. 139 nA mM À1) and limit of detection (24 mM) to glucose was obtained when a combination of GOPMOS with MTMOS sol -gel was used, but for a short linear range (0 -0.2 mM) and using a big concentration of immobilized enzyme (ca. 3.5%). The longest linear range (0 -1 mM) was obtained for the biosensor prepared with GOPMOS and TEOS sol -gel but with lower sensitivity (19.7 nA mM À1 ) and a bigger detection limit (71 mM) with a concentration of immobilized enzyme of 1%. The biosensors were used at 0.0 V vs. SCE with minimal interference from carboxylic acids and phenol; only ascorbate showed a significant interference but this effect was decreased to 3% by coating with Nafion polymer. The biosensor was successfully applied to the analysis of glucose in wine samples.

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Co-immobilization of cholesterol oxidase and horseradish peroxidase in a sol–gel film

Cited by 127 publications

References 20 publications

Carbon Nanotubes‐Based Amperometric Cholesterol Biosensor Fabricated Through Layer‐by‐Layer Technique

Carbon Nanotubes‐Based Amperometric Cholesterol Biosensor Fabricated Through Layer‐by‐Layer Technique

Influence of silane content on the optical properties of sol gel derived spin coated silica thin films

Development and Application of Oxysilane Sol–Gel Electrochemical Glucose Biosensors Based on Cobalt Hexacyanoferrate Modified Carbon Film Electrodes

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