Co-Immobilization of Glucose Dehydrogenase and Xylose Dehydrogenase as a New Approach for Simultaneous Production of Gluconic and Xylonic Acid

Abstract

The conversion of biomass components catalyzed via immobilized enzymes is a promising way of obtaining valuable compounds with high efficiency under mild conditions. However, simultaneous transformation of glucose and xylose into gluconic acid and xylonic acid, respectively, is an overlooked research area. Therefore, in this work we have undertaken a study focused on the co-immobilization of glucose dehydrogenase (GDH, EC 1.1.1.118) and xylose dehydrogenase (XDH, EC 1.1.1.175) using mesoporous Santa Barbara Amorphous silica (SBA 15) for the simultaneous production of gluconic acid and xylonic acid. The effective co-immobilization of enzymes onto the surface and into the pores of the silica support was confirmed. A GDH:XDH ratio equal to 1:5 was the most suitable for the conversion of xylose and glucose, as the reaction yield reached over 90% for both monosaccharides after 45 min of the process. Upon co-immobilization, reaction yields exceeding 80% were noticed over wide pH (7–9) and temperature (40–60 °C) ranges. Additionally, the co-immobilized GDH and XDH exhibited a significant enhancement of their thermal, chemical and storage stability. Furthermore, the co-immobilized enzymes are characterized by good reusability, as they facilitated the reaction yields by over 80%, even after 5 consecutive reaction steps.

Novel Bionanocompounds: Outer Membrane Protein A and Laccase Co-Immobilized on Magnetite Nanoparticles for Produced Water Treatment

“…The enzyme contents were estimated based on the enzymatic activity as described in Supplementary Materials, Section SI5 and Figure S4 . This approach followed previous reports where the number of active immobilized molecules has been estimated by quantifying their catalytic activity towards a substrate with the aid of colorimetric or fluorescent methods [ 69 , 74 , 75 , 76 ]. Figure 3 c shows the immobilization yield obtained according to content of Laccase.…”

Xylose Dehydrogenase Immobilized on Ferromagnetic Nanoparticles for Bioconversion of Xylose to Xylonic Acid

“…Bachosz et al co-immobilized xylose dehydrogenase and alcohol dehydrogenase onto silica core–shell for xylose conversion to xylonic acid and cofactor regeneration . Similarly, glucose dehydrogenase and xylose dehydrogenase were immobilized onto mesoporous amorphous silica for coproduction of gluconic acid and xylonic acid . Zdarta et al demonstrated enzymatic production of xylonic acid and gluconic acid from biomass-derived xylose and glucose via immobilization of xylose dehydrogenase and glucose dehydrogenase onto porous silica nanoparticles .…”

Co-immobilized recombinant glycosyltransferases efficiently convert rebaudioside A to M in cascade

“…26 In contrast, the two enzymes were xed in the same carrier bead in the dual-enzyme co-immobilization system would reduce the transport time of the substrate and intermediate loss due to diffusion, 52 the substrate was instantly converted to the nal product due to that two closely enzymes constituted a efficiently cascade biocatalyst. 28,[53][54][55] Therefore, this co-immobilization system enabled a fast transfer of substrate, thus enhancing the overall catalytic efficiency of the cascade reactions.…”