Co-incubation of human spermatozoa with Chlamydia trachomatis serovar E causes premature sperm death

Hosseinzadeh, Saeid

doi:10.1093/humrep/16.2.293

Cited by 89 publications

(73 citation statements)

References 27 publications

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“…LCS was associated with decreased sperm count, % normal forms and fructose concentration, a measure of seminal vesicle function. Thus, the present study confirms and extends earlier reports that STIs and LCS are associated with poor semen quality (7,13,45,48,49,51,64,65,70,(77)(78)(79)(80)(81)(82).…”

Section: Discussionsupporting

confidence: 92%

Prevalence of sexually transmissible pathogens in semen from asymptomatic male infertility patients with and without leukocytospermia

Bezold

Politch

Kiviat

et al. 2007

Fertility and Sterility

218

167

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Objective-To determine the prevalence of pathogens that cause sexually transmitted infections (STIs) in semen from asymptomatic male infertility patients with and without leukocytospermia (LCS), and associations between STIs, inflammatory markers and other semen variables.Design-Retrospective, controlled study. Results-STI DNA was detected in 45/241 (18.7%) of the samples (CMV 8.7%, HPV 4.5%, HHV-6 3.7%, HSV 3.7%, CT 2.5%, EBV 0.4%, and HBV 0%), with no difference in prevalence between LCS and non-LCS groups. STI DNA in semen was associated with a decrease in sperm concentration, motile sperm concentration, total sperm count and neutral α-glucosidase concentration, whereas LCS was associated with a decrease in total sperm count, % normal forms and fructose concentration. Setting-CenterReprint requests: Deborah J. Anderson, Ph.D., Department of Obstetrics and Gynecology, Boston University School of Medicine, 670 Albany Street, Suite 516, Boston, MA 02118, (FAX: 617-414-8481; e-mail: E-mail: deborah.anderson@bmc.org). Capsule: Sexually transmissible pathogens were detected in 19% of semen samples from infertility patients seeking routine semen analyses; their presence was not associated with leukocytospermia, but was associated with reduced semen quality.Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Conclusion(s)-STI pathogen DNA was detected in semen from a high percentage of asymptomatic male infertility patients and was associated with poor semen quality. Efforts to diagnose and treat subclinical genital tract infections should be intensified. NIH Public Access

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Section: Discussionsupporting

confidence: 92%

Prevalence of sexually transmissible pathogens in semen from asymptomatic male infertility patients with and without leukocytospermia

Bezold

Politch

Kiviat

et al. 2007

Fertility and Sterility

218

167

View full text Add to dashboard Cite

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“…The dead S. aureus neither adhered to spermatozoa nor induced any changes in the morphology (Figure 3b) which might be the reason of causing no immobilization of the spermatozoa. In an earlier study, Hosseinzadeh et al (2001) have also shown that live Chlamydial elementary bodies (EBs) can have a direct and detrimental effect on sperm physiology whereas heat treatment abolishes the same.…”

Section: Resultsmentioning

confidence: 99%

“…Among bacterial species that interact with spermatozoa are well known causative pathogens of genitourinary infections such as Escherichia coli, Ureaplasma urealyticum, Mycoplasma hominis and Chlamydia trachomatis (Kohn et al, 1998, Hosseinzadeh et al, 2001, Gdoura et al, 2007. E. coli probably represents the most frequently isolated microorganism in genitourinary infections (Weidner et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Interference of Human Spermatozoal Motility by Live Staphylococcus aureus

Kaur¹,

Prabha²,

Shukla³

2010

Am. J. Biomed. Sci.

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The aim of this work was to investigate the effect of live Staphylococcus aureus commonly present in the cervix of females on sperm motility and agglutination in vitro. Highly motile samples of spermatozoa (40 x 10 6 /ml) from normozoospermic males were co-incubated for 4h with 10 7 cells/ml of live S. aureus. After 30 minutes, 1, 2 and 4h of incubation, sperm motility and agglutination was checked by light microscopic examination (X 400). From the onset, it was apparent that the live bacteria reduced the motility of spermatozoa. The promptness with which the decreased motility was observed to occur in the live S. aureus group was striking. After 30 minutes of mixing the ejaculate with the live bacteria, decrease in motility and clumping of the spermatozoa was noted. Interestingly, no agglutination and immobilization was observed in other experiments in which the bacteria were killed by heat, lysozyme or antibiotics and mixed with the ejaculate. Certainly, it seems evident that live S. aureus can significantly depress the motility and agglutinate human spermatozoa in vitro. The mechanism by which this alteration takes place is not obvious, however, detrimental effect on sperm motility by live staphylococci may be an as yet unrecognized component of fertility problems.

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“…In vitro experiments have shown that C. trachomatis triggers tyrosine phosphorylation of sperm proteins (Hosseinzadeh et al, 2000), induces premature sperm death (Hosseinzadeh et al, 2001) and stimulates an apoptosis-like response in sperm (Eley et al, 2005b;Satta et al, 2006), leading to increased levels of sperm DNA fragmentation (Gallegos et al, 2008;Satta et al, 2006). At least some of these effects are caused by lipopolysaccharides (Hosseinzadeh et al, 2003).…”

Section: Infertilitymentioning

confidence: 99%