Co-infection of epithelial cells established from the upper and lower bovine respiratory tract with bovine respiratory syncytial virus and bacteria

Sudaryatma, Putu Eka; Mekata, Hirohisa; Kubo, Meiko; Subangkit, Mawar; Goto, Yoshitaka; Okabayashi, Tamaki

doi:10.1016/j.vetmic.2019.06.010

Cited by 11 publications

(26 citation statements)

References 32 publications

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“…In our previous studies, we demonstrated that BRSV regulates adherence of Pasteurella multocida (PM) to human and bovine respiratory epithelial cells (BRECs). Specifically, we showed that pre-infection of bovine bronchus epithelial cells (bBECs) and lung epithelial cells (bLECs) with BRSV increased adherence by PM; this effect was not seen for bovine trachea epithelial cells (bTECs) (Sudaryatma et al, 2019). Those results indicated that BRSV may reproduce better in the lower respiratory tract, leading in turn to increased adherence of bacteria.…”

Section: Introductionmentioning

confidence: 87%

“…Bovine respiratory epithelial cells were collected from three freshly slaughtered adult Japanese black cattle, as described previously (Sudaryatma et al, 2019). Briefly, epithelial cells were recovered from organ trachea (bTECs), bronchus (bBECs), and lung (bLECs).…”

Section: Cellsmentioning

confidence: 99%

“…Bovine respiratory syncytial virus strain 2205027-1 and P. multocida strain 2368 were prepared as described previously (Sudaryatma et al, 2019). For infection, virus or bacteria were diluted in antibiotic-and serum-free DMEM/F12 to achieve the desired multiplicity of infection target.…”

Section: Virus and Bacteriummentioning

confidence: 99%

“…Bovine respiratory epithelial cells were infected with BRSV as described previously (Sudaryatma et al, 2019). Briefly, epithelial cells from trachea (bTECs), bronchus (bBECs), and lung (bLECs) were seeded overnight and infected with infectious BRSV (MOI 0.1 or MOI 1) or UV-BRSV.…”

Section: Brsv Infection Of Brecsmentioning

confidence: 99%

“…Quantitative real-time RT-PCR assays (qRT-PCR) were performed as described previously (Sudaryatma et al, 2019). Briefly, viral RNA in the culture supernatant of BRSV-infected cells was extracted using the NucleoSpin Virus Kit (TaKaRa Bio, Japan) according to the manufacturer's protocol.…”

Section: Quantification Of Viral Rna In Brecsmentioning

confidence: 99%

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Bovine Respiratory Syncytial Virus Enhances the Adherence of Pasteurella multocida to Bovine Lower Respiratory Tract Epithelial Cells by Upregulating the Platelet-Activating Factor Receptor

et al. 2020

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Coinfection by bovine respiratory syncytial virus (BRSV) and Pasteurella multocida (PM) frequently has been observed in cattle that develop severe pneumonia. We recently reported that BRSV infection significantly increased PM adherence to bovine lower respiratory tract epithelial cells. However, the molecular mechanisms of enhanced PM adherence are not completely understood. To investigate whether BRSV infection regulates any cellular adherence receptors on bovine bronchus-and lung-epithelial cells, we performed proteomic and functional analyses. The proteomic analysis showed that BRSV infection increased the accumulation of the platelet-activating factor receptor (PAFR) in both cell types. Molecular experiments, including specific blockade, knockdown, and overexpression of PAFR, indicated that PM adherence to these cell types depended on PAFR expression. These findings highlight the role, in cattle with severe pneumonia, of the synergistic effect of coinfection by BRSV and PM in the lower respiratory tract.

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Section: Introductionmentioning

confidence: 87%

Section: Cellsmentioning

confidence: 99%

Section: Virus and Bacteriummentioning

confidence: 99%

Section: Brsv Infection Of Brecsmentioning

confidence: 99%

Section: Quantification Of Viral Rna In Brecsmentioning

confidence: 99%

See 3 more Smart Citations

Bovine Respiratory Syncytial Virus Enhances the Adherence of Pasteurella multocida to Bovine Lower Respiratory Tract Epithelial Cells by Upregulating the Platelet-Activating Factor Receptor

et al. 2020

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Serological profile of respiratory viruses in unvaccinated steers upon their arrival at Brazilian feedlot facilities

Chicoski,

Fritzen,

Lorenzetti

et al. 2023

Braz J Microbiol

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Bovine Respiratory Syncytial Virus Decreased Pasteurella multocida Adherence by Downregulating the Expression of Intercellular Adhesion Molecule-1 on the Surface of Upper Respiratory Epithelial Cells

Sudaryatma

Saito

Mekata

et al. 2020

Veterinary Microbiology

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The synergistic infection of bovine respiratory syncytial virus (BRSV) and Pasteurella multocida (PM) may predispose cattle to develop severe pneumonia. Previously, we reported that BRSV infection significantly decreased PM adherence to the upper respiratory epithelial cells. It may allow bacteria to invade into the lower respiratory tract and lead to severe pneumonia. To investigate whether BRSV infection regulates the cell surface adherence receptor on bovine trachea epithelial cells (bTECs), we performed proteomic and functional analyses. BRSV infection decreased the expression of intercellular adhesion molecule-1 (ICAM1) on bTECs. Inhibition and knockdown experiments using anti-ICAM1 antibody and siRNAs targeting ICAM1 indicated that PM adherence to bTECs was dependent on ICAM1 expression. These data suggest that under normal conditions bTECs may capture PM in the upper respiratory tract, while BRSV infection reverses this mechanism. The proposed gateway function of bTECs is disrupted by BRSV infection that may facilitate bacterial invasion into the lower respiratory tract and lead to secondary or more severe respiratory infection.

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Co-infection of epithelial cells established from the upper and lower bovine respiratory tract with bovine respiratory syncytial virus and bacteria

Cited by 11 publications

References 32 publications

Bovine Respiratory Syncytial Virus Enhances the Adherence of Pasteurella multocida to Bovine Lower Respiratory Tract Epithelial Cells by Upregulating the Platelet-Activating Factor Receptor

Bovine Respiratory Syncytial Virus Enhances the Adherence of Pasteurella multocida to Bovine Lower Respiratory Tract Epithelial Cells by Upregulating the Platelet-Activating Factor Receptor

Serological profile of respiratory viruses in unvaccinated steers upon their arrival at Brazilian feedlot facilities

Bovine Respiratory Syncytial Virus Decreased Pasteurella multocida Adherence by Downregulating the Expression of Intercellular Adhesion Molecule-1 on the Surface of Upper Respiratory Epithelial Cells

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