Co-inoculation of an Antibiotic-Producing Bacterium and a Lytic Enzyme-Producing Bacterium for the Biocontrol of Tomato Wilt Caused by Fusarium oxysporum f. sp. lycopersici

Someya, Nobutaka; Tsuchiya, Kenichi; Yoshida, Takeshi; Noguchi, Masako Tsujimoto; Akutsu, Katsumi; Sawada, Hiroyuki

doi:10.4265/bio.12.1

Cited by 26 publications

(13 citation statements)

References 24 publications

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“…However, in the presence of these crude preparations and prodigiosin from S. marcescens CAB 1094, we observed a significant decrease in the numbers of culturable yeast cells. These results support the findings of others who discovered that a number of fungi, including Didymella applanata [62], Fusarium oxysporum [65], Trichoderma reesei and Phycomyces blakesleeanus [56], are relatively resistant in vitro to the effect of S. marcescens chitinases. However, studies indicate that the co-inoculation of S. marcescens and Pseudomonas fluorescens enhances the antifungal activity of P. fluorescens towards F. oxysporum [65], and it was contented that this enhanced antifungal effect was a result of S. marcescens chitinase activity.…”

Section: Discussionsupporting

confidence: 93%

“…These results support the findings of others who discovered that a number of fungi, including Didymella applanata [62], Fusarium oxysporum [65], Trichoderma reesei and Phycomyces blakesleeanus [56], are relatively resistant in vitro to the effect of S. marcescens chitinases. However, studies indicate that the co-inoculation of S. marcescens and Pseudomonas fluorescens enhances the antifungal activity of P. fluorescens towards F. oxysporum [65], and it was contented that this enhanced antifungal effect was a result of S. marcescens chitinase activity. Similarly, researchers found that culture filtrates from S. marcescens show synergistic antifungal activity with a number of chemical fungicides against sclerotial viability of Rhizoctonia solani [64].…”

Section: Discussionsupporting

confidence: 93%

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Binary Interactions of Antagonistic Bacteria with Candida albicans Under Aerobic and Anaerobic Conditions

et al. 2015

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We used both aerobic and anaerobic liquid co-cultures, prepared with Luria Bertani broth, to study the effect of bacteria on the survival of Candida albicans in the external environment, away from an animal host. The bacteria were represented by Aeromonas hydrophila, Bacillus cereus, Bacillus subtilis, Clostridium, Enterobacter, Klebsiella pneumoniae, Kluyvera ascorbata and Serratia marcescens. Under aerobic conditions, the yeast's growth was inhibited in the presence of bacterial growth; however, under anaerobic conditions, yeast and bacterial growth in co-cultures was similar to that observed for pure cultures. Subsequent assays revealed that the majority of bacterial strains aerobically produced extracellular hydrolytic enzymes capable of yeast cell wall hydrolysis, including chitinases and mannan-degrading enzymes. In contrast, except for the A. hydrophila strain, these enzymes were not detected in anaerobic bacterial cultures, nor was the antimicrobial compound prodigiosin found in anaerobic cultures of S. marcescens. When we suspended C. albicans cells in crude extracellular enzyme preparations from K. pneumoniae and S. marcescens, we detected no negative effect on yeast viability. However, we found that these preparations enhance the toxicity of prodigiosin towards the yeast, especially in combination with mannan-degrading enzymes. Analyses of the chitin and mannan content of yeast cell walls revealed that less chitin was produced under anaerobic than aerobic conditions; however, the levels of mannan, known for its low permeability, remained the same. The latter phenomenon, as well as reduced production of the bacterial enzymes and prodigiosin, may contribute to anaerobic growth and survival of C. albicans in the presence of bacteria.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Binary Interactions of Antagonistic Bacteria with Candida albicans Under Aerobic and Anaerobic Conditions

et al. 2015

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“…24 isolates were positive for lipase, 19 each for protease and xylanase, 14 for amylase, 11 for pectinase, and 9 for cellulase ( Figure 1(b)). Production of extracellular cell wall degrading enzymes has been associated with biocontrol abilities in plant growth promoting microbes [13,37]. The influence due to the presence of plant growth promoting microbes was demonstrable when the soil was used in form of inoculum representing a "consortium" of beneficial microbes.…”

Section: Resultsmentioning

confidence: 99%

Microbial Diversity in Soil under Potato Cultivation from Cold Desert Himalaya, India

2013

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Mana village (Chamoli district, Uttarakhand, India), situated in high altitudes (3,238 m above mean sea level) of Indian Himalayan region, represents cold desert climatic conditions. At Mana, potato is grown from May to September, while the site remains snow clad for approximately six months (from October to April). Soil samples, collected from Mana potato fields, were analyzed for cultivable microbial diversity along with the chemical and enzymatic properties. The analysis revealed colonization of soil by microflora in moderate numbers (up to 10 7 CFU/g soil) with limited species level. 25 morphologically distinct microbial isolates belonging to Gram +ve and Gram −ve bacteria, actinomycetes, and fungi including yeast were isolated. The bacteria were tentatively identified as species of Bacillus and Pseudomonas, while the majority of the fungal isolates belonged to the species of Penicillium. These microbial isolates possessed plant growth promotion and biocontrol properties assessed mainly in terms of production of indole acetic acid and hydrolytic enzymes and phosphate solubilization. The soil, when used as "inoculum" in plant based bioassays, exhibited positive influence on plant growth related parameters. The limited diversity of cold tolerant microbial species also extends opportunity to understand the resilience possessed by these organisms under low temperature environment.

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“…These enzymes were also identified at high levels in the laticifer cells of rubber tree 101 . Microbial chitinases have been shown to exhibit antifungal activity and used for biological control against plant fungal diseases 102–105 . Chitinases of insect origin also exhibit toxic effects on other insects upon oral ingestion 106, 107 .…”

Section: Discussionmentioning

confidence: 99%

Comparative morphology and transcriptome analysis reveals distinct functions of the primary and secondary laticifer cells in the rubber tree

et al. 2017

Sci Rep

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Laticifers are highly specialized cells that synthesize and store natural rubber. Rubber trees (Hevea brasiliensis Muell. Arg.) contain both primary and secondary laticifers. Morphological and functional differences between the two types of laticifers are largely unknown, but such information is important for breeding and cultivation practices. Morphological comparison using paraffin sections revealed only distribution differences: the primary laticifers were distributed randomly, while the secondary laticifers were distributed in concentric rings. Using isolated laticifer networks, the primary laticifers were shown to develop via intrusive “budding” and formed necklace-like morphology, while the secondary laticifers developed straight and smooth cell walls. Comparative transcriptome analysis indicated that genes involved in cell wall modification, such as pectin esterase, lignin metabolic enzymes, and expansins, were highly up-regulated in the primary laticifers and correspond to its necklace-like morphology. Genes involved in defense against biotic stresses and rubber biosynthesis were highly up-regulated in the primary laticifers, whereas genes involved in abiotic stresses and dormancy were up-regulated in the secondary laticifers, suggesting that the primary laticifers are more adequately prepared to defend against biotic stresses, while the secondary laticifers are more adequately prepared to defend against abiotic stresses. Therefore, the two types of laticifers are morphologically and functionally distinct.

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Co-inoculation of an Antibiotic-Producing Bacterium and a Lytic Enzyme-Producing Bacterium for the Biocontrol of Tomato Wilt Caused by Fusarium oxysporum f. sp. lycopersici

Cited by 26 publications

References 24 publications

Binary Interactions of Antagonistic Bacteria with Candida albicans Under Aerobic and Anaerobic Conditions

Binary Interactions of Antagonistic Bacteria with Candida albicans Under Aerobic and Anaerobic Conditions

Microbial Diversity in Soil under Potato Cultivation from Cold Desert Himalaya, India

Comparative morphology and transcriptome analysis reveals distinct functions of the primary and secondary laticifer cells in the rubber tree

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