Co-localization of NG2 proteoglycan and PDGF ?-receptor on O2A progenitor cells in the developing rat brain

Abstract

A detailed comparison in the developing rat central nervous system between the distribution of the NG2 proteoglycan and the α‐receptor for platelet‐derived growth factor (PDGF) shows that these two molecules are co‐expressed by glial progenitor cells of the O2A lineage and can serve as reliable markers for identification of O2A cells in vivo. Our mapping experiments indicate that NG2‐positive, PDGF α‐receptor positive O2A cells are abundant throughout the developing central nervous system in both white and gray matter. The earliest cells immunoreactive for either of the two markers are found adjacent to the central canal of the embryonic day 15 (E15) spinal cord. These cells express only PDGF α‐receptor and not NG2. By E17, process‐bearing cells expressing both NG2 and PDGF α‐receptor in a highly co‐localized fashion are found throughout the central nervous system. The first postnatal week marks the peak in the number of NG2 and PDGF α‐receptor immunoreactive cells, as well as the peak in the level of expression and the extent of co‐localization of the two molecules. After the first week, the level of expression of both NG2 and PDGF α‐receptor declines, although both molecules continue to be expressed in the adult brain. On O2A cells in the mature brain, NG2 and PDGF α‐receptor are not as well co‐localized at the subcellular level as they are on O2A cells in the younger brain. The functional consequences of co‐localization and subsequent dissociation of NG2 and PDGF α‐receptor on maturing O2A progenitors are investigated in the accompanying paper (Nishiyama et al.: J Neurosci Res 43:315–330, 1996). © 1996 Wiley‐Liss, Inc.

Expression of Neural RNA-Binding Proteins in the Postnatal CNS: Implications of Their Roles in Neuronal and Glial Cell Development

“…3G-J, arrows), indicating that astrocytes retain expression of m-Msi-1, whereas it is not expressed in differentiated or immature neurons and oligodendrocytes. Some antigenic markers have been used for analysis of the sequential differentiation of oligodendrocytes from dividing precursors, including O-2A progenitor cells, into quiescent oligodendrocytes in vitro and in vivo (Hardy and Reynolds, 1991;Nishiyama et al, 1996). PDGF␣-R is expressed in the developing and adult rodent CNS by the O-2A oligodendrocyte progenitors and preoligodendrocytes (Pringle et al, 1992;Ellison and de Vellis, 1994;Nishiyama et al, 1996).…”

“…Some antigenic markers have been used for analysis of the sequential differentiation of oligodendrocytes from dividing precursors, including O-2A progenitor cells, into quiescent oligodendrocytes in vitro and in vivo (Hardy and Reynolds, 1991;Nishiyama et al, 1996). PDGF␣-R is expressed in the developing and adult rodent CNS by the O-2A oligodendrocyte progenitors and preoligodendrocytes (Pringle et al, 1992;Ellison and de Vellis, 1994;Nishiyama et al, 1996). Similarly, NG2, a core membrane protein associated with chondroitin sulfate proteoglycan, is also expressed in mitotic O-2A progenitor cells localized in the gray and white matter of perinatal to adult rat brain and disappears as these cells differentiate into oligodendrocytes (Stallcup and Beasley, 1987;Nishiyama et al, 1996).…”

Stable Methylation at Promoters Distinguishes Epiblast Stem Cells from Embryonic Stem Cells and the In Vivo Epiblasts

“…Of these, Abcb1a is a membrane transporter whereas the others are involved in development [41]. Chrna3, Cspg4, Daam2, and Gfra3 play roles in nervous system development [42][43][44][45][46], while Aebp1 is required for smooth muscle formation [47][48][49][50]. We verified the low level of DNA methylation in two different ESC lines, which contrasted with the high DNA methylation level (90%-98% methylated CpGs) in two EpiSC lines (Fig.…”

Oligodendrocyte progenitor cells can act as cell of origin for experimental glioma

“…Studies on experimental glioma using the RCAS/tv-a model have shown that neural/glial stem cells in Ntv-a mice and neural stem cells/astrocytes in Gtv-a mice have the capacity to generate gliomas Holland and Vermus, 1998). The majority of induced tumors in both Ntv-a and Gtv-a mice were positive for NG2 chondroitin sulfate proteoglycan (NG2), a marker of early oligodendrocyte progenitor cells (OPCs) (Stallcup and Beasley, 1987;Pringle and Richardson, 1993;Nishiyama et al, 1996;Polito and Reynolds, 2005). This implied that the OPCs could represent the optimal level of cellular differentiation for oncogenic transformation to occur.…”