Co-localization of PTEN and E-cadherin in canine mammary hyperplasias and benign and malignant mammary tumors

Abstract

Fifty-four canine mammary lesions (15 hyperplasias, 7 adenomas and 32 carcinomas) were submitted to immunohistochemical analysis for the evaluation of PTEN and E-cadherin co-expression. Subjects bearing mammary carcinomas were also submitted to a 2-year follow-up study to compare immunohistochemical results with overall survival. All the hyperplastic samples stained positive for both markers, 100% of adenomas were positive for PTEN and 86% for E-cadherin, and 69% and 34% of carcinomas were positive for PTEN and E-cadherin, respectively. Statistical analysis showed a positive correlation between these two proteins both considering all (p b 0.01) or malignant tumors (p < 0.05). The female dogs bearing tumors positively-stained for both markers had a longer overall survival (p < 0.05) and absence of lymphatics invasion (p < 0.05). Simultaneous double immunofluorescence confirmed the co-localization of the two proteins in neoplastic cells. Results reported in this study confirm the tumor suppressor effect of these two molecules.

Promotive effect of Talin‐1 protein on gastric cancer progression through PTK2‐PXN‐VCL‐E‐Cadherin‐CAPN2‐MAPK1 signaling axis

“…E‐cadherin protein is an important tumor metastasis inhibited gene involving in maintaining the normal epithelium integrity and polarity. When the cell integrity is destroyed and the polarity is disturbed, the tumor cells become more active and their activity range is enlarged, which makes it easier for tumor cells to break away from the primary site to invade and metastasize 9 . CAPN2 protein is involved in cytoskeleton remodeling and signal transduction, while MAPK1 is involved in cell proliferation and differentiation 10 .…”

Specific cellular biomarkers for canine s breast cancer: literature review

“…The undifferentiated canine's mammary carcinomas, both invasive and metastatic, may present loss of E-cadherin expression in some subpopulations of tumor cells, when compared to well differentiated carcinoma, suggesting that altered expression of E-cadherin could be an important process for malignant transformation. This hypothesis is supported by the observation that loss of E-cadherin expression is correlated with a shorter overall survival and free of diseases (Klopfleisch et al, 2011;Asproni et al, 2015). P-cadherin is a glycoprotein with a structure similar to cadherin.…”

“…In dogs, there is a significant correlation between the expression of P-cadherin and the high degree of the tumor, suggesting that their aberrant expression is a marker of biologic aggressiveness of these tumors. In addition, aberrant expression of P-cadherin in these tumors is associated with the infiltrative growth pattern of the tumor (Walls et al, 2007;Klopfleisch et al, 2011;Asproni et al, 2015).…”

Current invasive and non-invasive biomarkers in canine mammary tumors

“…Immunohistochemical analysis exhibited that E-cadherin membrane expression reduces in canine mammary carcinoma (Asproni et al, 2015). This result was associated with aggressive clinical features observed in canine mammary carcinomas such as high mitotic index, large tumor size, infiltration, invasion, and lymph node metastasis (Brunetti et al, 2003;Matos et al, 2006;.…”