Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb

Zheng, Ning; Wang, Zhizhong; Wang, Songwei; Yang, Fangjia; Zhu, Xutao; Chen, Lü; Manyande, Anne; Rao, Xiaoping; Xu, Fuqiang

doi:10.24272/j.issn.2095-8137.2020.020

Cited by 6 publications

(2 citation statements)

References 31 publications

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“…The MeA receives direct projections from the AOB (Zheng et al., 2020). We therefore wanted to examine whether Foxp2 ‐ and Dbx1 ‐lineage neurons in the AOB project to the MeA.…”

Section: Resultsmentioning

confidence: 99%

Connectivity and molecular profiles of Foxp2‐ and Dbx1‐lineage neurons in the accessory olfactory bulb and medial amygdala

Prakash,

Matos,

Sebaoui

et al. 2023

J of Comparative Neurology

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In terrestrial vertebrates, the olfactory system is divided into main (MOS) and accessory (AOS) components that process both volatile and nonvolatile cues to generate appropriate behavioral responses. While much is known regarding the molecular diversity of neurons that comprise the MOS, less is known about the AOS. Here, focusing on the vomeronasal organ (VNO), the accessory olfactory bulb (AOB), and the medial amygdala (MeA), we reveal that populations of neurons in the AOS can be molecularly subdivided based on their ongoing or prior expression of the transcription factors Foxp2 or Dbx1, which delineate separate populations of GABAergic output neurons in the MeA. We show that a majority of AOB neurons that project directly to the MeA are of the Foxp2 lineage. Using single‐neuron patch‐clamp electrophysiology, we further reveal that in addition to sex‐specific differences across lineage, the frequency of excitatory input to MeA Dbx1‐ and Foxp2‐lineage neurons differs between sexes. Together, this work uncovers a novel molecular diversity of AOS neurons, and lineage and sex differences in patterns of connectivity.

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“…The MeA receives direct projections from the AOB (Zheng et al., 2020). We therefore wanted to examine whether Foxp2 ‐ and Dbx1 ‐lineage neurons in the AOB project to the MeA.…”

Section: Resultsmentioning

confidence: 99%

Connectivity and molecular profiles of Foxp2‐ and Dbx1‐lineage neurons in the accessory olfactory bulb and medial amygdala

Prakash,

Matos,

Sebaoui

et al. 2023

J of Comparative Neurology

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“…The vomeronasal sensory neurons (VSNs) are distributed in the vomeronasal epithelium of the VNO, and their targets are in glomeruli in the accessory olfactory bulb (AOB) (Firestein, 2001;Halpern and Martinez-Marcos, 2003;Yokosuka, 2012). The secondary and tertiary centers of the VNO include the bed nucleus of stria terminalis (BNST), bed nucleus of the accessory olfactory tract, the posteroventral medial amygdala (MePV) and posterodorsal medial amygdala (MePD) (Mohedano-Moriano et al, 2007;Yokosuka, 2012;Zheng et al, 2020). In contrast, the GG is a part of main olfactory system (MOS), and is an arrow-shaped neuronal structure and situates underneath the dorsal cartilage of the anterior tip of the nasal cavity.…”

Section: Introductionmentioning

confidence: 99%

Neuropeptide S Attenuates the Alarm Pheromone-Evoked Defensive and Risk Assessment Behaviors Through Activation of Cognate Receptor-Expressing Neurons in the Posterior Medial Amygdala

Shao

Wang

Rao

et al. 2021

Front. Mol. Neurosci.

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Neuropeptide S (NPS) acts by activating its cognate receptor (NPSR). High level expression of NPSR in the posterior medial amygdala suggests that NPS-NPSR system should be involved in regulation of social behaviors induced by social pheromones. The present study was undertaken to investigate the effects of central administration of NPS or with NPSR antagonist on the alarm pheromone (AP)-evoked defensive and risk assessment behaviors in mice. Furthermore, H129-H8, a novel high-brightness anterograde multiple trans-synaptic virus, c-Fos and NPSR immunostaining were employed to reveal the involved neurocircuits and targets of NPS action. The mice exposed to AP displayed an enhancement in defensive and risk assessment behaviors. NPS (0.1–1 nmol) intracerebroventricular (i.c.v.) injection significantly attenuated the AP-evoked defensive and risk assessment behaviors. NPSR antagonist [D-Val5]NPS at the dose of 40 nmol completely blocked the effect of 0.5 nmol of NPS which showed the best effective among dose range. The H129-H8-labeled neurons were observed in the bilateral posterodorsal medial amygdala (MePD) and posteroventral medial amygdala (MePV) 72 h after the virus injection into the unilateral olfactory bulb (OB), suggesting that the MePD and MePV receive olfactory information inputs from the OB. The percentage of H129-H8-labeled neurons that also express NPSR were 90.27 ± 3.56% and 91.67 ± 2.46% in the MePD and MePV, respectively. NPS (0.5 nmol, i.c.v.) remarkably increased the number of Fos immunoreactive (-ir) neurons in the MePD and MePV, and the majority of NPS-induced Fos-ir neurons also expressed NPSR. The behavior characteristic of NPS or with [D-Val5]NPS can be better replicated in MePD/MePV local injection within lower dose. The present findings demonstrated that NPS, via selective activation of the neurons bearing NPSR in the posterior medial amygdala, attenuates the AP-evoked defensive and risk assessment behaviors in mice.

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Longitudinal neural connection detection using a ferritin‐encoding adeno‐associated virus vector and in vivo MRI method

Cai

Zheng

Thompson

et al. 2021

Human Brain Mapping

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The investigation of neural circuits is important for interpreting both healthy brain function and psychiatric disorders. Currently, the architecture of neural circuits is always investigated with fluorescent protein encoding neurotropic virus and ex vivo fluorescent imaging technology. However, it is difficult to obtain a whole-brain neural circuit connection in living animals, due to the limited fluorescent imaging depth.Herein, the noninvasive, whole-brain imaging technique of MRI and the hypotoxicity virus vector AAV (adeno-associated virus) were combined to investigate the wholebrain neural circuits in vivo. AAV2-retro are an artificially-evolved virus vector that permits access to the terminal of neurons and retrograde transport to their cell bodies. By expressing the ferritin protein which could accumulate iron ions and influence the MRI contrast, the neurotropic virus can cause MRI signal changes in the infected regions. For mice injected with the ferritin-encoding virus vector (rAAV2-retro-CAG-Aoling Cai and Ning Zheng contributed equally to this work.

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Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb

Cited by 6 publications

References 31 publications

Connectivity and molecular profiles of Foxp2‐ and Dbx1‐lineage neurons in the accessory olfactory bulb and medial amygdala

Connectivity and molecular profiles of Foxp2‐ and Dbx1‐lineage neurons in the accessory olfactory bulb and medial amygdala

Neuropeptide S Attenuates the Alarm Pheromone-Evoked Defensive and Risk Assessment Behaviors Through Activation of Cognate Receptor-Expressing Neurons in the Posterior Medial Amygdala

Longitudinal neural connection detection using a ferritin‐encoding adeno‐associated virus vector and in vivo MRI method

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