Co-localization of vasoactive intestinal polypeptide, γ-aminobutyric acid and choline acetyltransferase in neocortical interneurons of the adult rat

Bayraktar, Tan; Staiger, Jochen F.; Acsády, László; Cozzari, Costantino; Freund, Tamás F.; Zilles, Karl

doi:10.1016/s0006-8993(97)00218-7

Cited by 99 publications

(103 citation statements)

References 7 publications

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“…5G,H ). In VIP, ChAT, and 5-HT triple-labeled sections (n ϭ 230 cells from 3 rats), 40.1 Ϯ 2.7% of VIP neurons coexpress ChAT in line with previous reports (Chédotal et al, 1994a;Bayraktar et al, 1997). Although there was no difference between VIP and VIP/ChAT cell bodies or dendrites in their innervation by 5-HT afferents, a significantly greater proportion of VIP/ChAT than VIP cells was contacted by ACh varicosities on both their somata (35.5 vs 24.5%; p Ͻ 0.05) and dendrites (39.8 vs 22.1%; p Ͻ 0.05) (Fig.…”

Section: Vip Neuronssupporting

confidence: 90%

Cortical GABA Interneurons in Neurovascular Coupling: Relays for Subcortical Vasoactive Pathways

Cauli¹,

Tong²,

Rancillac³

et al. 2004

J. Neurosci.

518

530

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Section: Vip Neuronssupporting

confidence: 90%

Cortical GABA Interneurons in Neurovascular Coupling: Relays for Subcortical Vasoactive Pathways

Cauli¹,

Tong²,

Rancillac³

et al. 2004

J. Neurosci.

518

530

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“…The results of the present study showed that, in ferrets, virtually all vagal preganglionic neurons innervating trachea and intrapulmonary airways are cholinergic in nature and coexpress VIP, indicating that acetylcholine and neuropeptide VIP are coexisting messenger molecules in AVPNs. Contrary to AVPNs, only one-third of all cortical intrinsic cholinergic neurons contain immunoreactivity for VIP (5,11).…”

Section: Discussionmentioning

confidence: 85%

Chemical profile of vagal preganglionic motor cells innervating the airways in ferrets: the absence of noncholinergic neurons

Mayer

Haxhiu

2004

Journal of Applied Physiology

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Kc, Prabha, Catherine A. Mayer, and Musa A. Haxhiu. Chemical profile of vagal preganglionic motor cells innervating the airways in ferrets: the absence of noncholinergic neurons. J Appl Physiol 97: 1508 -1517, 2004; 10.1152/japplphysiol.00282.2004.-In ferrets, we investigated the presence of choline acetyltransferase (ChAT), vasoactive intestinal peptide (VIP), and markers for nitric oxide synthase (NOS) in preganglionic parasympathetic neurons innervating extrathoracic trachea and intrapulmonary airways. Cholera toxin ␤-subunit, a retrograde axonal transganglionic tracer, was used to identify airway-related vagal preganglionic neurons. Double-labeling immunohistochemistry and confocal microscopy were employed to characterize the chemical nature of identified airway-related vagal preganglionic neurons at a single cell level. Physiological experiments were performed to determine whether activation of the VIP and ChAT coexpressing vagal preganglionic neurons plays a role in relaxation of precontracted airway smooth muscle tone after muscarinic receptor blockade. The results showed that 1) all identified vagal preganglionic neurons innervating extrathoracic and intrapulmonary airways are acetylcholine-producing cells, 2) cholinergic neurons innervating the airways coexpress ChAT and VIP but do not contain NOS, and 3) chemical stimulation of the rostral nucleus ambiguus had no significant effect on precontracted airway smooth muscle tone after muscarinic receptor blockade. These studies indicate that vagal preganglionic neurons are cholinergic in nature and coexpress VIP but do not contain NOS; their stimulation increases cholinergic outflow, without activation of inhibitory nonadrenergic, noncholinergic ganglionic neurons, stimulation of which induces airway smooth muscle relaxation. Furthermore, these studies do not support the possibility of direct inhibitory innervation of airway smooth muscle by vagal preganglionic fibers that contain VIP.central nervous system control of the airways; parasympathetic preganglionic neurons; choline acetyl transferase; vasoactive intestinal peptide; nitric oxide synthase; nucleus ambiguus; airway constriction; airway dilation IN MANY SPECIES, INCLUDING HUMANS, airway smooth muscle (ASM) tone critically relies on the balance between central excitatory cholinergic control (3,19,33,49) and the inhibitory nonadrenergic, noncholinergic (iNANC) influences (2,12,17,47,56). Activation of cholinergic preganglionic neurons produces airway constriction, submucosal gland secretion and increased blood flow using acetylcholine as a neurotransmitter (23,25,26). In the presence of cholinergic and sympathetic blockade, stimulation of vagal nerves, either by electrical current or via neural reflex pathways, elicits relaxation of preconstricted ASM cells via activation of iNANC pathways (2,12,17,56). Recent studies indicate that the iNANC system employs vasoactive intestinal peptide (VIP) (7,16,22,38,47,57), nitric oxide (NO) (4, 30, 36, 44, 51), or both (6, 14 -16, 64) to elicit ASM relaxation. Ho...

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“…Thus, the presence of calretinin (Suárez-Solá et al, 2009), calbindin (Yan et al, 1996), VIP (Bayraktar et al, 1997(Bayraktar et al, , 2000, NPY (Adrian et al, 1983;Chan-Palay et al, 1985), cholecystokinin (Innis et al, 1979), substance P (Shults et al, 1984), somatostatin (Somogyi et al, 1984), and nNOS (Sandell, 1986;Tao et al, 1999; Suárez-Solá et al, 2009) was reported for WMICs. Of note, in the transgenic mice employed here, EGFP expression in the corpus callosum was confined by and large to VIP-, calretinin-, and calbindin-positive interneurons.…”

Section: Discussionmentioning

confidence: 96%

“…Basic morphological features of WMICs were indicative of a neuronal phenotype (Neuburger, 1922;Lund et al, 1975;Kostovic and Rakic, 1980;Chun and Shatz, 1989;Muller, 1994;Shering and Lowenstein, 1994;Eastwood and Harrison, 2005;Friedlander and Torres-Reveron, 2009;Loup et al, 2009), a notion that was also supported by electron microscopy data (Valverde and Facal-Valverde, 1988). While there is ample information about the immunohistochemical signature of WMICs, such as the expression of GABA and specific GABAergic interneuron markers (Innis et al, 1979;Adrian et al, 1983;Schmechel et al, 1984;Shults et al, 1984;Somogyi et al, 1984;Chan-Palay et al, 1985;Sandell, 1986;Yan et al, 1996;Bayraktar et al, 1997;Tao et al, 1999;Suárez-Solá et al, 2009), only a few studies focused on electrophysiological properties of WMICs (Clancy et al, 2001;Torres-Reveron and Friedlander, 2007;Friedlander and Torres-Reveron, 2009). The main hindrance for the identification of WMICs in acute slices is their scarcity.…”

Section: Introductionmentioning

confidence: 93%

5-HT_3AReceptor-Bearing White Matter Interstitial GABAergic Interneurons Are Functionally Integrated into Cortical and Subcortical Networks

et al. 2011

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In addition to axons and surrounding glial cells, the corpus callosum also contains interstitial neurons that constitute a heterogeneous cell population. There is growing anatomical evidence that white matter interstitial cells (WMICs) comprise GABAergic interneurons, but so far there is little functional evidence regarding their connectivity. The scarcity of these cells has hampered electrophysiological studies. We overcame this hindrance by taking recourse to transgenic mice in which distinct WMICs expressed enhanced green fluorescence protein (EGFP). The neuronal phenotype of the EGFP-labeled WMICs was confirmed by their NeuN positivity. The GABAergic phenotype could be established based on vasoactive intestinal peptide and calretinin expression and was further supported by a firing pattern typical for interneurons. Axons and dendrites of many EGFP-labeled WMICs extended to the cortex, hippocampus, and striatum. Patch-clamp recordings in acute slices showed that they receive excitatory and inhibitory input from cortical and subcortical structures. Moreover, paired recordings revealed that EGFP-labeled WMICs inhibit principal cells of the adjacent cortex, thus providing unequivocal functional evidence for their GABAergic phenotype and demonstrating that they are functionally integrated into neuronal networks.

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Co-localization of vasoactive intestinal polypeptide, γ-aminobutyric acid and choline acetyltransferase in neocortical interneurons of the adult rat

Cited by 99 publications

References 7 publications

Cortical GABA Interneurons in Neurovascular Coupling: Relays for Subcortical Vasoactive Pathways

Cortical GABA Interneurons in Neurovascular Coupling: Relays for Subcortical Vasoactive Pathways

Chemical profile of vagal preganglionic motor cells innervating the airways in ferrets: the absence of noncholinergic neurons

5-HT_3AReceptor-Bearing White Matter Interstitial GABAergic Interneurons Are Functionally Integrated into Cortical and Subcortical Networks

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Co-localization of vasoactive intestinal polypeptide, γ-aminobutyric acid and choline acetyltransferase in neocortical interneurons of the adult rat

Cited by 99 publications

References 7 publications

Cortical GABA Interneurons in Neurovascular Coupling: Relays for Subcortical Vasoactive Pathways

Cortical GABA Interneurons in Neurovascular Coupling: Relays for Subcortical Vasoactive Pathways

Chemical profile of vagal preganglionic motor cells innervating the airways in ferrets: the absence of noncholinergic neurons

5-HT3AReceptor-Bearing White Matter Interstitial GABAergic Interneurons Are Functionally Integrated into Cortical and Subcortical Networks

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5-HT_3AReceptor-Bearing White Matter Interstitial GABAergic Interneurons Are Functionally Integrated into Cortical and Subcortical Networks