Co-occurrence of antibiotic and disinfectant resistance genes in extensively drug-resistant Escherichia coli isolated from broilers in Ilorin, North Central Nigeria

Al-Mustapha, Ahmad Ibrahim; Alada, Shafi Abdullah; Ibrahim, Rasheed Bolaji; Lawal, Nurudeen Adedeji; Eskola, Katarina; Brouwer, Michael S.M.; Adetunji, Victoria O.; Heikinheimo, Annamari

doi:10.1016/j.jgar.2022.11.002

Cited by 6 publications

(2 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the 15 antibiotics used in this study, all E. coli strains were resistant to at least five types of antibiotics, which is similar to the results of a study by Zhang et al who strained more than 95% of E. coli as multidrug-resistant bacteria in marketed retail food in 39 cities in China [49]. Moreover, alarming MDR has also been observed in previous studies from broiler chicken farms in other countries [50][51][52]. Therefore, there is a need for increased surveillance of antimicrobial resistance in E. coli to prevent the further spread of multidrug-resistant E. coli.…”

Section: Discussionsupporting

confidence: 90%

Characteristics, Whole-Genome Sequencing and Pathogenicity Analysis of Escherichia coli from a White Feather Broiler Farm

Wu,

Cui,

Han

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Avian colibacillosis, caused by avian Escherichia coli (E. coli), has historically been one of the most prevalent infectious diseases in large-scale poultry production, causing growth delays and mortality in chickens, resulting in huge economic losses. In recent years, the widespread use of antibiotics has led to the emergence of multidrug resistance in E. coli as a significant global problem and long-term challenge. Resistant E. coli can be transmitted to humans through animal products or the environment, which presents significant public health concerns and food safety issues. In this study, we analyzed the features of 135 E. coli strains obtained from a white feather broiler farm in Shandong, China, including antimicrobial susceptibility tests, detection of class 1 integrons, drug resistance genes, virulence genes, and phylogenetic subgroups. It is particularly worrying that all 135 E. coli strains were resistant to at least five antibiotic agents, and 100% of them were multidrug-resistant (MDR). Notably, the resistance genes of blaTEM, blaCTX-M, qnrS, aaC4, tetA, and tetB exhibited a high prevalence of carriage among the tested resistance genes. However, mcr-2~mcr-9 were not detected, while the prevalence of mcr-1 was found to be 2.96%. The most common virulence genes detected were EAST1 (14.07%, encoding enterotoxins) and fyuA (14.81%, encoding biofilm formation). Phylogenetic subgroup analysis revealed that E. coli belonging to groups B2 and D, which are commonly associated with high virulence, constituted 2.22% and 11.11%, respectively. The positive rate of class 1 integrons was 31.1%. Whole-genome sequencing (WGS) and animal experiments were performed on a unique isolated strain called 21EC78 with an extremely strong membrane-forming capacity. The WGS results showed that 21EC78 carried 11 drug resistance genes and 16 virulence genes. Animal experiments showed that intraperitoneal injection with 2 × 105 CFU could cause the death of one-day-old SPF chickens in 3 days. However, the mortality of Luhua chickens was comparatively lower than that of SPF chickens. This study reports the isolation of multidrug-resistant E. coli strains in poultry, which may pose a potential threat to human health via the food chain. Furthermore, the findings of this study enhance our comprehension of the frequency and characteristics of multidrug-resistant E. coli in poultry farms, emphasizing the urgent need for improved and effective continuous surveillance to control its dissemination.

show abstract

Section: Discussionsupporting

confidence: 90%

Characteristics, Whole-Genome Sequencing and Pathogenicity Analysis of Escherichia coli from a White Feather Broiler Farm

Wu,

Cui,

Han

et al. 2023

Microorganisms

View full text Add to dashboard Cite

show abstract

“…The higher the effective chlorine content, the stronger the disinfection effect. However, frequent use of high-concentration disinfectants for disinfection may result in disinfectant resistance [ 21 , 22 ]. Moreover, chlorine-containing disinfectant has corrosive and bleaching effects and can irritate the skin [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

The effect of different concentrations of chlorine-containing disinfectants on high-frequency contact table in intensive care units: A quasi-experimental study

Zhang

et al. 2023

PLoS ONE

View full text Add to dashboard Cite

Background The hospital environment, particularly the intensive care unit (ICU), contributes to the transmission of several nosocomial pathogens, which can survive in this setting for a longer period of time and, in turn, contaminate the surfaces or the medical tools. Thus, appropriate disinfection of these areas and devices are crucial for controlling and preventing further infection. In this study, we examined the effect of different concentrations of chlorine-containing disinfectants (500mg/L, 1000mg/L, and 2000mg/L) on the ICU environment. Methods This quasi-experimental study was based on a convenient sampling method. In this study, High-frequency objects were selected as subjects in ICU, with a total sample of 216.A hall including 6 beds was examined,selecting 4 high-frequency surfaces per bed unit:a bed gear, infusion system, bed end table, and monitor were disinfected with 500, 1000, and 2000 mg/L of chlorine (as Cl2), respectively.The surface dissection was performed at 21:00 o’clock daily, after which ATP fluorescence monitoring and bacterial count detection were performed. Results There was no significant difference in ATP bioluminescence (F = 2.03, P > 0.05) and bacterial counting (χ2 = 2.03, P > 0.05) when using different concentrations of chlorine-containing disinfectant in the ICU. Yet, compared with high concentration (2000mg/L), a low concentration disinfectant reduced the hospital cost. Conclusion By reducing the concentration of ICU high-frequency contact table disinfectants, it is possible to reduce the risk of long-term contamination with chlorine-containing disinfectants and reduce the cost of using ICU chlorine-containing disinfectants.

show abstract

Antibiotic resistance genes, mobile elements, virulence genes, and phages in cultivated ESBL-producing Escherichia coli of poultry origin in Kwara State, North Central Nigeria

Al-Mustapha

Raufu

Ogundijo

et al. 2023

International Journal of Food Microbiology

View full text Add to dashboard Cite

Co-occurrence of antibiotic and disinfectant resistance genes in extensively drug-resistant Escherichia coli isolated from broilers in Ilorin, North Central Nigeria

Cited by 6 publications

References 52 publications

Characteristics, Whole-Genome Sequencing and Pathogenicity Analysis of Escherichia coli from a White Feather Broiler Farm

Characteristics, Whole-Genome Sequencing and Pathogenicity Analysis of Escherichia coli from a White Feather Broiler Farm

The effect of different concentrations of chlorine-containing disinfectants on high-frequency contact table in intensive care units: A quasi-experimental study

Antibiotic resistance genes, mobile elements, virulence genes, and phages in cultivated ESBL-producing Escherichia coli of poultry origin in Kwara State, North Central Nigeria

Contact Info

Product

Resources

About