Co-Occurrence of Colistin and Meropenem Resistance Determinants in a <i>Stenotrophomonas</i> Strain Isolated from Sewage Water

Li, Jun; Liu, Shuyan; Fu, Jiafang; Yin, Jianhua; Zhao, Jia; Zhong, Chunlong; Cao, Guangxiang

doi:10.1089/mdr.2018.0418

Cited by 30 publications

(28 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…www.nature.com/scientificreports www.nature.com/scientificreports/ newly reported in Raoultella spp. 24 was also identified and appears to be present in Stenotrophomonas spp.. As a confirmation of this hypothesis, this latter has been recently described in Stenotrophomonas strain from sewage water 30 . Interestingly, whilst MCR-8 has only been reported in less than five studies to date, we identified 22 MCR-8 in already sequenced genomes of Klebsiella and Raoultella spp.. All these newly described MCR variants are real true witnesses of our results which predict putative 5'265 MCR-like sequences that could emerged soon in common pathogenic bacteria.…”

Section: Discussionsupporting

confidence: 70%

Massive analysis of 64,628 bacterial genomes to decipher water reservoir and origin of mobile colistin resistance genes: is there another role for these enzymes?

Khedher

Baron

Riziki

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Since 2015, new worrying colistin resistance mechanism, mediated by mcr-1 gene has been reported worldwide along with eight newly described variants but their source(s) and reservoir(s) remain largely unexplored. Here, we conducted a massive bioinformatic analysis of bacterial genomes to investigate the reservoir and origin of mcr variants. We identified 13'658 MCR-1 homologous sequences in 494 bacterial genera. Moreover, analysis of 64'628 bacterial genomes (60 bacterial genera and 1'047 species) allows identifying a total of 6'651 significant positive hits (coverage >90% and similarity >50%) with the nine MCR variants from 39 bacterial genera and more than 1'050 species. A high number of MCR-1 was identified in Escherichia coli (n = 862). Interestingly, while almost all variants were identified in bacteria from different sources (i.e. human, animal, and environment), the last variant, MCR-9, was exclusively detected in bacteria from human. Although these variants could be identified in bacteria from human and animal sources, we found plenty MCR variants in unsuspected bacteria from environmental origin, especially from water sources. The ubiquitous presence of mcr variants in bacteria from water likely suggests another role in the biosphere of these enzymes as an unknown defense system against natural antimicrobial peptides and/or bacteriophage predation.

show abstract

Section: Discussionsupporting

confidence: 70%

Massive analysis of 64,628 bacterial genomes to decipher water reservoir and origin of mobile colistin resistance genes: is there another role for these enzymes?

Khedher

Baron

Riziki

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…15 was also identified and appears to be present in Stenotrophomonas spp. As a confirmation of this hypothesis, this latter has been recently described in Stenotrophomonas strain from sewage water 34 . Interestingly, whilst MCR-8 has only been reported in less than five studies to date, we identified 22 MCR-8 in already sequenced genomes of Klebsiella and Raoultella spp.…”

Section: Discussionsupporting

confidence: 66%

“…It has recently been reported that the aquatic bacteria of the genus Shewanella are a reservoir/progenitor for mcr -4 encoding gene 47 . MCR-5 and MCR-8 were described from the same Stenotrophomonas strain isolated from sewage water in China 34 . Overall, urban wastewater treatment plants are one of the most common reservoir and source of antibiotic resistance genes (ARGs) against various antibiotic classes (β-lactams, aminoglycosides, quinolones, tetracyclines, sulfonamides, macrolides) 48 .…”

Section: Discussionmentioning

confidence: 99%

Massive analysis of 64’628 bacterial genomes to decipher a water reservoir and origin of mobile colistin resistance (mcr) gene variants: is there another role for this family of enzymes?

Khedher

Baron

Riziki

et al. 2019

Preprint

View full text Add to dashboard Cite

20Since 2015, new worrying colistin resistance mechanism, mediated by mcr-1 21 gene has been reported worldwide along with eight newly described variants (mcr-2 to mcr-9) 22 but their source(s) and reservoir(s) remain largely unexplored. Here, we conducted a massive 23 bioinformatic analysis of 64'628 downloaded bacterial genomes to investigate the reservoir 24 and origin of these mcr variants. We identified a total of 6'651 significant positive hits (aa 25 sequence coverage > 90 % and similarity >50%) with the nine MCR variants from these 26 genomes that include 39 bacterial genera and more than 1050 species. Although these variants 27 could be identified in bacteria from human and animal sources, we found plenty MCR 28 variants in unsuspected bacteria from environmental origin, especially from water sources. 29The ubiquitous presence of mcr variants in bacteria from water likely suggests another role in 30 the biosphere of these enzymes as an unknown defense system against natural antimicrobial 31 peptides and/or bacteriophage predation. 32 21.Ling, Z. et al. Chromosome-mediated mcr-3 variants in aeromonas veronii from chicken meat. 440Antimicrob. Agents Chemother. 61, (2017). 441 22. Huang, M. et al. Action and mechanism of the colistin resistance enzyme MCR-4. Commun. 442 Biol. 2, (2019).443 23. Carroll, L. M. et al. Identification of Novel Mobilized Colistin Resistance Gene mcr-9 in a 595Suppl. Figure S2. Phylogenetic tree performed from MCR variant hits (n=6'648 proteins) with aa identity between 50% and 100% and 596 alignment ≥ 90%. 597

show abstract

“…Restrict treatment choices are feasible for the therapy of infections related to S. maltophilia isolates as a result of its instinctive resistance to various antibiotics owning to many kinds of β-lactamase or aminoglycosides, especially it is resistant to certain carbapenem and colistin antibiotics (25,26). In view of a systematic review and meta-analysis of non-randomized researches, trimethoprim-sulfamethoxazole manifested commensurate outcomes on rehabilitation of S. maltophilia infection to fluoroquinolones that is considered to be the most effective antibiotics for S. maltophilia (27).…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance Surveillance of Clinical Isolates of Stenotrophomonas maltophilia Strains in the Central South of China from 2016 to 2019

Yang

Yan

Chen

et al. 2020

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Due to the difficulty of treatment caused by its numerous mechanisms of resistance, only four kinds of antibiotics are recommended for the therapy of Stenotrophomonas maltophilia infections associated with significant morbidity and mortality. Objectives: In this four-year study, we aimed to determine the evolution of drug resistance to S. maltophilia base on drug classification guidelines recommended by the Institute of Clinical and Laboratory Standards. Methods: A total of 1876 strains of S. maltophilia was separated from multifarious clinical specimens among January 2016 and December 2019. VITEK 2 Compact microbial system was used for speciation level identification and antibiotic sensitivity test. Results: A total of 1876 strains of S. maltophilia strains were separated from sputum specimen type (70.63%), Followed by bronchial (6.18%), blood (4.16%), and bronchoalveolar lavage samples (4.32%). Moreover, 695 strains of S. maltophilia strains were separated from intensive care unit (ICU) department (37.05%), and then neurosurgery ward (10.66%), integrative Chinese and western medicine ward (7.25%), general surgery ward (6.66%). The results of minocycline antibiotic of S. maltophilia with a drug resistance rate of 0.3%. From 2016 to 2019, the resistance rate of cefoperazone/sulbactam decreased from 20.8% to 15.2%, the resistance rate of trimethoprimsulfametoxasole decreased from 7.9% to 4.5%. The resistance rate of minocycline fluctuate in 0.0%% between 0.7%. However, the resistance rate of levofloxacin increased from 7.7% to 8.0%. Conclusions: In this study, S. maltophilia was detected in a variety of specimen types of different clinical departments, with the most detected in ICU patients and sputum specimen. S. maltophilia was sensitive to minocycline and levofloxacin, but the situation of cefoperazone/sulbactam resistance was not optimistic. The results of this study indicate that minocycline is considered to be the most effective antibiotic for the treatment of S. maltophilia. Therefore, we still need to strengthen the drug resistance monitoring, timely acquisition of S. maltophilia drug resistance changes, and actively take effective measures to deal with the drug resistance of S. maltophilia.

show abstract

Co-Occurrence of Colistin and Meropenem Resistance Determinants in a Stenotrophomonas Strain Isolated from Sewage Water

Cited by 30 publications

References 40 publications

Massive analysis of 64,628 bacterial genomes to decipher water reservoir and origin of mobile colistin resistance genes: is there another role for these enzymes?

Massive analysis of 64,628 bacterial genomes to decipher water reservoir and origin of mobile colistin resistance genes: is there another role for these enzymes?

Massive analysis of 64’628 bacterial genomes to decipher a water reservoir and origin of mobile colistin resistance (mcr) gene variants: is there another role for this family of enzymes?

Antibiotic Resistance Surveillance of Clinical Isolates of Stenotrophomonas maltophilia Strains in the Central South of China from 2016 to 2019

Contact Info

Product

Resources

About