Co-overexpression of genes for nitrogen transport, assimilation, and utilization boosts rice grain yield and nitrogen use efficiency

Luo, Jie; HANG, Jun-nan; Wu, Bilong; Wei, Xilin; Zhao, Quanzhi; Fang, Zhongming

doi:10.1016/j.cj.2023.01.005

Cited by 15 publications

(5 citation statements)

References 66 publications

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“…The following steps refer to Luo et al (2023). 0.5 g of dry leaf blade, leaf sheath or rice powder was added to 0.5 g of copper sulfate pentahydrate and 4.5 g potassium sulfate, and then 8 mL of concentrated sulfuric acid was added.…”

Section: Total Nitrogen Measurementmentioning

confidence: 99%

Blocking of awn development‐related gene OsGAD1 coordinately boosts yield and quality of Kam Sweet Rice

Luo,

Amin,

et al. 2024

Physiologia Plantarum

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Kam Sweet Rice is a high‐quality local variety of Guizhou province in China, but most varieties have awns on lemma. In this study, we aimed to obtain awnless varieties of Kam Sweet Rice by blocking the awn development‐related gene OsGAD1 using CRISPR/Cas9 technology. We determined that natural variations of the OsGAD1 triggered different lengths of awns of Kam Sweet Rice. We found that the awning rate of the CRISPR lines of OsGAD1 in Guxiangnuo, Goujingao and Gouhuanggang decreased by over 65%, and the number of grains per panicle and yield per plant increased by more than 17% and 20% compared to the wild‐types. Furthermore, we indicated that blocking OsGAD1 resulted in an increase of over 2% in the brown rice rate and milled rice rate in these varieties. In addition, the analysis of the transcriptome revealed that the regulation of awn development and yield formation in CRISPR lines of OsGAD1 may involve genes associated with phytohormone and nitrogen pathways. These results suggest that blocking OsGAD1 in Kam Sweet Rice using CRISPR/Cas9 technology can be used for breeding programs seeking high yield and grain quality of Kam Sweet Rice.

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Section: Total Nitrogen Measurementmentioning

confidence: 99%

Blocking of awn development‐related gene OsGAD1 coordinately boosts yield and quality of Kam Sweet Rice

Luo,

Amin,

et al. 2024

Physiologia Plantarum

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“…Another instance is the GeMo rice which enhances the expression of genes that code for root cell nutrient transporters. 18 , 77 , 78 , 90 , 91 The GeMo rice plants are capable of absorbing and transferring nutrients more effectively, increasing yields and improving nutritional quality. This is achieved by increasing the activity of these transporters.…”

Section: Case Studies and Examples Of Using Genetic Intervention To I...mentioning

confidence: 99%

The recent genetic modification techniques for improve soil conservation, nutrient uptake and utilization

Sadikiel Mmbando,

Ngongolo

2024

GM Crops & Food

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“…ZmDof1 overexpression elevates N and C levels with improved photosynthetic rate and biomass in transgenic rice (Kurai et al, 2011). Additionally, co-overexpression of the N transport, assimilation, and utilization genes (OsAMT1;2, OsNPF8.9a, The Plant Genome OsNR2, and OsAS1) increases rice grain yield and NUE (Luo et al, 2023). In connection with this, it was revealed that the overexpression of OsAMT1;2 promoted an increase in rice biomass and NH 4 + uptake under NH 4 + -limited conditions (Konishi et al, 2021).…”

Section: Transgenic Approachesmentioning

confidence: 99%

Mineral nutrients in plants under changing environments: A road to future food and nutrition security

et al. 2023

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Plant nutrition is an important aspect that contributes significantly to sustainable agriculture, whereas minerals enrichment in edible source implies global human health; hence, both strategies need to be bridged to ensure “One Health” strategies. Abiotic stress‐induced nutritional imbalance impairs plant growth. In this context, we discuss the molecular mechanisms related to the readjustment of nutrient pools for sustained plant growth under harsh conditions, and channeling the minerals to edible source (seeds) to address future nutritional security. This review particularly highlights interventions on (i) the physiological and molecular responses of mineral nutrients in crop plants under stressful environments; (ii) the deployment of breeding and biotechnological strategies for the optimization of nutrient acquisition, their transport, and distribution in plants under changing environments. Furthermore, the present review also infers the recent advancements in breeding and biotechnology‐based biofortification approaches for nutrient enhancement in crop plants to optimize yield and grain mineral concentrations under control and stress‐prone environments to address food and nutritional security.

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Co-overexpression of genes for nitrogen transport, assimilation, and utilization boosts rice grain yield and nitrogen use efficiency

Cited by 15 publications

References 66 publications

Blocking of awn development‐related gene OsGAD1 coordinately boosts yield and quality of Kam Sweet Rice

Blocking of awn development‐related gene OsGAD1 coordinately boosts yield and quality of Kam Sweet Rice

The recent genetic modification techniques for improve soil conservation, nutrient uptake and utilization

Mineral nutrients in plants under changing environments: A road to future food and nutrition security

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