Co-overexpression of two Heat Shock Factors results in enhanced seed longevity and in synergistic effects on seedling tolerance to severe dehydration and oxidative stress

Personat, José-María; Tejedor-Cano, Javier; Prieto‐Dapena, Pilar; Almoguera, Concepción; Jordano, Juan

doi:10.1186/1471-2229-14-56

Cited by 53 publications

(42 citation statements)

References 38 publications

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“…During embryogenesis, seed cells experience large changes in structure and composition that are coincident with changes in cellular responses to desiccation. Numerous gene products are hypothesized to confer protection during desiccation and storage, for example, structural proteins and house-keeping or regulatory genes (Mouillon et al 2008;Mène-Saffrané et al 2010;Chatelain et al 2012;Verdier et al 2013;Personat et al 2014;Dekkers et al 2015).…”

Section: Response To Desiccation Stress From a Structural Perspectivementioning

confidence: 99%

Orthodoxy, recalcitrance and in-between: describing variation in seed storage characteristics using threshold responses to water loss

Walters¹

2015

Planta

171

167

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Discrete categories of seed physiology can be explained through a unified concept of the structural and molecular mobility responses within cells to drying. Tolerance of desiccation is typically described by a threshold or low water content limit to survival. This convention provides fairly good distinction between orthodox and recalcitrant seeds, which show thresholds of less than about 0.07 and greater than about 0.2 g H2O g DW(-1), respectively. Threshold water contents, however, are not direct measures of the intensity of water stress tolerated by seeds, nor are they measures of cell response to water stress. More direct criteria, that accommodate both spatial and temporal effects of water loss, are required to explain variation of desiccation tolerance and longevity in seeds from diverse genetic backgrounds and growth conditions. This essay presents the argument that changes in cellular volume directly quantify primary responses to desiccating stress in a context that also links damage, as cellular constituents compress, and protection, as compressed molecules form stabilizing structure. During desiccation, fluid cytoplasm solidifies, and the newly formed spatial relationships among molecules determine whether and how long viability is maintained. The diversity of seed behaviors suggests complexity and opportunity to discover molecules and mechanisms that regulate survival and perception of time in cells that lack metabolic function.

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Section: Response To Desiccation Stress From a Structural Perspectivementioning

confidence: 99%

Orthodoxy, recalcitrance and in-between: describing variation in seed storage characteristics using threshold responses to water loss

Walters¹

2015

Planta

171

167

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“…According to Huang et al (2012), desiccation tolerance loss in maize seeds is also associated with a reduction in the content of hydrophilic proteins during germination. In a study with sunflowers, Personat et al (2014) verified that the simultaneous overexpression of two genes that transcribes heat-stable protein, increased the tolerance to desiccation, reaching to drastic levels of dehydration.…”

Section: Heat-stable Proteinsmentioning

confidence: 99%

Physiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germination

2017

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During germination, orthodox seeds become gradually intolerant to desiccation, and for this reason, they are a good model for recalcitrance studies. In the present work, physiological, biochemical, and ultrastructural aspects of the desiccation tolerance were characterized during the germination process of Anadenanthera colubrina seeds. The seeds were imbibed during zero (control), 2, 8, 12 (no germinated seeds), and 18 hours (germinated seeds with 1 mm protruded radicle); then they were dried for 72 hours, rehydrated and evaluated for survivorship. Along the imbibition, cytometric and ultrastructural analysis were performed, besides the extraction of the heat-stable proteins. Posteriorly to imbibition and drying, the evaluation of ultrastructural damages was performed. Desiccation tolerance was fully lost after root protrusion. There was no increase in 4C DNA content after the loss of desiccation tolerance. Ultrastructural characteristics of cells from 1mm roots resembled those found in the recalcitrant seeds, in both hydrated and dehydrated states. The loss of desiccation tolerance coincided with the reduction of heat-stable proteins.Keywords: desiccation tolerance, forest seeds, drying.Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação ResumoDurante a germinação, sementes ortodoxas tornam-se gradualmente intolerantes à dessecação, e por isso podem ser utilizadas como modelo para o estudo da recalcitrância. No presente trabalho realizou-se uma caracterização dos aspectos fisiológicos, bioquímicos e ultraestruturais da perda da tolerância à dessecação de sementes de Anadenanthera colubrina em processo germinativo. Para isso as sementes foram embebidas durante 0 (controle), 2,8,12 e aproximadamente 18 horas (sementes germinadas com 1 mm de radícula), secas por 72 horas, reidratadas e a sobrevivência avaliada. Ao longo da embebição foram realizadas análises citométricas, ultraestruturais e extração de proteínas resistentes ao calor e após embebição e secagem foram avaliados danos ultraestruturais. A tolerância à dessecação foi totalmente perdida após a protrusão radicular. Não houve aumento do conteúdo de DNA 4C quando a tolerância à dessecação foi perdida. Características ultraestruturais de células de radículas de 1 mm assemelharam-se às encontradas em sementes recalcitrantes tanto no estado hidratado quanto desidratado. A perda da tolerância à dessecação coincidiu com a redução do conteúdo de proteínas resistentes ao calor.Palavras-chave: tolerância à dessecação, sementes floretais, secagem.

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“…Limiting factors for successful transformation include prolonged tissue culture time, low rate of plant regeneration, precocious flowering, poor rooting, and abnormal morphogenesis (Radonic et al 2006;Sujatha et al 2012). As a result, only a few silencing or overexpression experiments have been reported for this crop (Hu et al 2003;Manavella and Chan 2009;Guan et al 2014) and most of the functional studies of sunflower genes have relied on the use of heterologous systems, particularly Arabidopsis thaliana (Dezar et al 2005;Manavella et al 2006;Manavella et al 2008;Manning et al 2008;Dezar et al 2011;Personat et al 2014). In addition to the advantages associated with the use of model species, A. thaliana has been demonstrated to provide a suitable system to study plant-pathogen interactions for both biotrophic and necrotrophic fungi, including S. sclerotiorum (Dai et al 2006;Guo and Stotz 2007;Perchepied et al 2010;Foley et al 2013).…”

Section: Introductionmentioning

confidence: 98%

Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana

et al. 2015

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The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection against Sclerotinia sclerotiorum and Rhizoctonia solani. Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoproteins belonging to the Cupin super family. These proteins have been widely studied because of their diverse roles in important plant processes, including defence. The novel sunflower gene HaGLP1 encodes the first germin-like protein characterized from the family Asteraceae. To analyse whether constitutive in vivo expression of the HaGLP1 gene may lead to disease tolerance, we developed transgenic Arabidopsis plants that were molecularly characterized and biologically assessed after inoculation with Sclerotinia sclerotiorum or Rhizoctonia solani. HaGLP1 expression in Arabidopsis plants conferred tolerance to S. sclerotiorum at the first stages of disease and interfered with R. solani infection, thus giving rise to significant protection against the latter. Furthermore, HaGLP1 expression in Arabidopsis plants elevated endogenous ROS levels. HaGLP1-induced tolerance does not appear to be related to a constitutive induction of the plant defence or the ROS-related genes examined here. In conclusion, our data suggest that HaGLP1 is an interesting candidate for the engineering of plants with increased fungal tolerance and that this gene could also be useful for the selection of naturally overexpressing sunflower genotypes for conventional breeding purposes.

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Co-overexpression of two Heat Shock Factors results in enhanced seed longevity and in synergistic effects on seedling tolerance to severe dehydration and oxidative stress

Cited by 53 publications

References 38 publications

Orthodoxy, recalcitrance and in-between: describing variation in seed storage characteristics using threshold responses to water loss

Orthodoxy, recalcitrance and in-between: describing variation in seed storage characteristics using threshold responses to water loss

Physiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germination

Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana

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