Co-Overexpression of Two Key Source Genes, <i>OsBMY4</i> and <i>OsISA3</i>, Improves Multiple Key Traits of Rice Seeds

Wang, Zhen; Zhou, Yu; Ren, Xinyu; Wei, Ke; Fan, Xiaolei; Huang, Li‐Chun; Zhao, Dongsheng; Zhang, Lin; Zhang, Changquan; Liu, Qiaoquan

doi:10.1021/acs.jafc.2c06039

Cited by 17 publications

(13 citation statements)

References 50 publications

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“…In this study, RNA‐seq analysis revealed that the Osga2ox5 mutant shows altered expression of numerous genes involved in carbohydrate metabolism. For example, OsISA3 , which has been shown to play an essential role in improving seed germination under drought stress (Wang et al., 2023), was down‐regulated in the Osga2ox5 mutant. Collectively, these findings demonstrated that OsGA2ox5 might regulate drought tolerance during seed germination by modulating GA homeostasis and subsequently influencing carbohydrate metabolism.…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide association studies for rice seed germination under drought stress using 3VmrMLM

Yang,

Chen,

Zheng

et al. 2024

Food and Energy Security

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Improving seed germination under drought stress has the potential to increase crop yield in dry direct‐seeded rice. In this study, a genome‐wide association study (GWAS) was conducted to determine the genetic basis of seed germination under drought stress in a panel of 165 rice accessions using a multi‐locus compressed variance‐component mixed model, 3VmrMLM. A total of 33 quantitative trait nucleotides (QTNs) were identified in association with drought tolerance during seed germination. Around these QTNs, eight were found to be co‐localized with known drought‐related genes. Furthermore, a causal candidate gene, OsGA2ox5, which encodes a gibberellin (GA) 2‐oxidase, was identified for QTN‐Chr07_242906 by integrating linkage disequilibrium (LD) analysis and gene expression analysis. Knockout mutation of OsGA2ox5 resulted in delayed seed germination and retarded seedling growth under drought stress. RNA‐seq analysis revealed that OsGA2ox5 regulates drought tolerance during seed germination mainly by modulating carbohydrate metabolism. Additionally, a non‐synonymous coding variant in OsGA2ox5, SNP‐Chr07_218893, was found to be strongly associated with drought tolerance level during seed germination. These findings provide valuable information for further exploration of novel drought‐related genes and genetic improvement of rice drought tolerance in the future.

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Section: Discussionmentioning

confidence: 99%

Genome‐wide association studies for rice seed germination under drought stress using 3VmrMLM

Yang,

Chen,

Zheng

et al. 2024

Food and Energy Security

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“…According to reports, GH family-related genes in grapes promote the synthesis of β-amylase, which promotes starch degradation ( Zhou et al, 2023 ). In addition, overexpression of the gene of GH family in rice could accelerate starch degradation efficiency and increase the sugar supply for sink organs ( Wang et al, 2022 ). Therefore, the upregulated expression of LOC107771686, LOC107773095, LOC107774582 and LOC107798981 in BS3 treatment group colud accelerate starch degradation.…”

Section: Discussionmentioning

confidence: 99%

Screening, identification, and mechanism analysis of starch-degrading bacteria during curing process in tobacco leaf

Zhang,

Jiang,

et al. 2024

Front. Bioeng. Biotechnol.

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Tobacco, a vital economic crop, had its quality post-curing significantly influenced by starch content. Nonetheless, the existing process parameters during curing were inadequate to satisfy the starch degradation requirements. Microorganisms exhibit inherent advantages in starch degradation, offering significant potential in the tobacco curing process. Our study concentrated on the microbial populations on the surface of tobacco leaves and in the rhizosphere soil. A strain capable of starch degradation, designated as BS3, was successfully isolated and identified as Bacillus subtilis by phylogenetic tree analysis based on 16SrDNA sequence. The application of BS3 on tobacco significantly enhanced enzyme activity and accelerated starch degradation during the curing process. Furthermore, analyses of the metagenome, transcriptome, and metabolome indicated that the BS3 strain facilitated starch degradation by regulating surface microbiota composition and affecting genes related to starch hydrolyzed protein and key metabolites in tobacco leaves. This study offered new strategies for efficiently improving the quality of tobacco leaves.

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“…The thermodynamic properties of rice were determined using a differential scanning calorimeter (DSC 200 F3, Netzsch Instruments NA LLC; Burlington, MA) [ 49 ]. The viscosity of rice was measured using a Rapid Visco Analyzer (RVA) (Techmaster; Newport Scientific, Australia), and the data obtained were analyzed using the TCE software [ 50 ].…”

Section: Methodsmentioning

confidence: 99%

RGA1 regulates grain size, rice quality and seed germination in the small and round grain mutant srg5

Yang,

Lu,

Shi

et al. 2024

BMC Plant Biol

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Background Generating elite rice varieties with high yield and superior quality is the main goal of rice breeding programs. Key agronomic traits, including grain size and seed germination characteristics, affect the final yield and quality of rice. The RGA1 gene, which encodes the α-subunit of rice G-protein, plays an important role in regulating rice architecture, seed size and abiotic stress responses. However, whether RGA1 is involved in the regulation of rice quality and seed germination traits is still unclear. Results In this study, a rice mutant small and round grain 5 (srg5), was identified in an EMS-induced rice mutant library. Systematic analysis of its major agronomic traits revealed that the srg5 mutant exhibited a semi-dwarf plant height with small and round grain and reduced panicle length. Analysis of the physicochemical properties of rice showed that the difference in rice eating and cooking quality (ECQ) between the srg5 mutant and its wild-type control was small, but the appearance quality was significantly improved. Interestingly, a significant suppression of rice seed germination and shoot growth was observed in the srg5 mutant, which was mainly related to the regulation of ABA metabolism. RGA1 was identified as the candidate gene for the srg5 mutant by BSA analysis. A SNP at the splice site of the first intron disrupted the normal splicing of the RGA1 transcript precursor, resulting in a premature stop codon. Additional linkage analysis confirmed that the target gene causing the srg5 mutant phenotype was RGA1. Finally, the introduction of the RGA1 mutant allele into two indica rice varieties also resulted in small and round rice grains with less chalkiness. Conclusions These results indicate that RGA1 is not only involved in the control of rice architecture and grain size, but also in the regulation of rice quality and seed germination. This study sheds new light on the biological functions of RGA1, thereby providing valuable information for future systematic analysis of the G-protein pathway and its potential application in rice breeding programs.

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Co-Overexpression of Two Key Source Genes, OsBMY4 and OsISA3, Improves Multiple Key Traits of Rice Seeds

Cited by 17 publications

References 50 publications

Genome‐wide association studies for rice seed germination under drought stress using 3VmrMLM

Genome‐wide association studies for rice seed germination under drought stress using 3VmrMLM

Screening, identification, and mechanism analysis of starch-degrading bacteria during curing process in tobacco leaf

RGA1 regulates grain size, rice quality and seed germination in the small and round grain mutant srg5

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