Co-production of Amylase and Protease by Locally Isolated Thermophilic Bacterium Anoxybacillus rupiensis T2 in Sterile and Non-sterile Media Using Waste Potato Peels as Substrate

Tuysuz, Elanur; Gonul-Baltaci, Nurdan; Omeroglu, Mehmet Akif; Adıgüzel, Ahmet; Taşkın, Mesut; Özkan, Hakan

doi:10.1007/s12649-020-00936-3

Cited by 24 publications

(6 citation statements)

References 31 publications

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“…In addition, the accumulation of certain products of enzymatic reactions leads to a repression effect [ 34 ]. Many studies use a variety of organic sources as a substrate in enzyme production such as wheat bran, oat flour production, and potato peel powder [ [34] , [35] , [36] , [37] ]. Indeed, the production of enzymes is inducible and strongly affected by the types of carbon sources (induced or repressed).…”

Section: Resultsmentioning

confidence: 99%

Stable and effective eco-enzyme cocktails in powder and liquid form of Stachybotrys microspora used as detergent additives

Ben Hmad,

Gargouri

2024

Heliyon

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Section: Resultsmentioning

confidence: 99%

Stable and effective eco-enzyme cocktails in powder and liquid form of Stachybotrys microspora used as detergent additives

Ben Hmad,

Gargouri

2024

Heliyon

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“…Furthermore, the ability of the isolates to produce two or more enzymes suggests that there might be synergistic activities between these enzymes on the overall metabolism of the cell. Hence, co-production of these two enzymes is being pursued [2][3] . These also suggest that the isolates from this study which were able to produce both enzymes may be tapped in the co-production of amylase and protease.…”

Section: Pharmaceutical Sciences Asiamentioning

confidence: 99%

“…Owing to their conditions, soils harbor a diverse plethora of microbial species, thereby resulting in a wide range of enzymes with varying characteristics 2 . Among these species, the genus Bacillus has been widely regarded as "factory" of different bioactive compounds including enzymes 3 . Some of the commonly produced enzymes of Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Screening for production of amylase and protease by locally-isolated Bacillus spp. from soil collected in Taguig City and Clark Freeport Zone, Philippines

Guzman¹,

Mantaring²,

Panerio³

2022

Pharm Sci Asia

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Amylase and protease are two of the most commonly used enzymes in the pharmaceutical industry. Particularly, Bacillus spp. is widely regarded as a "factory" of these enzymes. In this study, a total of 40 isolates were collected from four soil samples from different metropolitan sites namely, near Chemicals and Energy Division (CED), and National Metrology Laboratory (NML) oil tankers parking lot inside the Department of Science and Technology (DOST) Compound in Taguig City, and two metropolitan-volcanic sites in Clark Freeport Zone, Pampanga (CRK1, CRK2). Of these, 33 isolates were deemed putative Bacillus spp. via phenotypic assays and were screened for the production of amylase and protease. Results showed that 26 of the screened isolates were able to produce protease and 12 were positive for amylase production. Molecular identification revealed that the enzyme-producing isolates were Bacillus spp., B. cereus, B. aryabhattai, B. amyloliquefaciens, B. firmus, B. velezensis, and Fictibacillus sp. No isolate was able to produce amylase alone. These results show the potential of Bacillus spp. from metropolitan soil as sources of pharmaceutically-important enzymes.

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“…Producing different enzymes from distinct sources is not cost-effective for any industry that needs multiple enzymes [14]. Hence, the concomitant production of two or more enzymes by microbial fermentation is an adroit and putative technique which can reduce time, energy and labor as well [15]. From the literature survey, we found that laccase and lignin peroxidase [16], amylase and xylanase [17], cellulase and xylanase [18], amylase, lipase and protease [19], protease and amylase [5], xylanase and alkaline protease [20], endoglucanase and xylanase [21], amylase and protease [15], pectinase, cellulase and xylanase [22] were few of the coproduced enzymes through bacterial submerged fermentation.…”

Section: Introductionmentioning

confidence: 99%

Agarase, Amylase and Xylanase from Halomonas meridiana: A Study on Optimization of Coproduction for Biomass Saccharification

Veerakumar

Manian

2022

Fermentation

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Coproduction of multienzymes from single potential microbe has captivated contemplation in industries. Bacterial strain, Halomonas meridiana VITSVRP14, isolated from seaweed was labored to produce amylase, agarase and xylanase conjointly using submerged fermentation. The optimum production conditions clinched by classical optimization were: pH 8; 1.5% inoculum; 24 h incubation, 40 °C; 8% NaCl (sodium chloride); 1% lactose and NaNO3 (sodium nitrate). The preponderant variables (pH, temperature, lactose) and their interaction effect on enzyme production were studied by Plackett-Burman design and Response Surface Methodology (RSM). There were 3.29, 1.81 and 2.08 fold increase in enzyme activity with respect to agarase, amylase and xylanase after optimization against basal medium. After 24 h of enzymatic treatment, the saccharification rates of the coproduced enzyme mixture were 38.96% on rice bran, 49.85% on wheat bran, 61.2% on cassava bagasse and 57.82% on corn cob. Thus, the coproduced enzyme mixture from a bacterium with halotolerance is plausible in pretreated lignocellulose degradation. The ability of this single microbe Halomonas meridiana VITSVRP14, in coproducing agarase, amylase and xylanase give the nod for its application in biomass saccharification by subsiding cost, energy and time involved in the process.

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Co-production of Amylase and Protease by Locally Isolated Thermophilic Bacterium Anoxybacillus rupiensis T2 in Sterile and Non-sterile Media Using Waste Potato Peels as Substrate

Cited by 24 publications

References 31 publications

Stable and effective eco-enzyme cocktails in powder and liquid form of Stachybotrys microspora used as detergent additives

Stable and effective eco-enzyme cocktails in powder and liquid form of Stachybotrys microspora used as detergent additives

Screening for production of amylase and protease by locally-isolated Bacillus spp. from soil collected in Taguig City and Clark Freeport Zone, Philippines

Agarase, Amylase and Xylanase from Halomonas meridiana: A Study on Optimization of Coproduction for Biomass Saccharification

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