Co-production of ethanol-hydrogen by genetically engineered Escherichia coli in sustainable biorefineries for lignocellulosic ethanol production

Lopez-Hidalgo, Angel Mario; Magaña, G.; Rodríguez, Fernando Martín; León‐Rodríguez, Antonio De; Sánchez, Arturo

doi:10.1016/j.cej.2020.126829

Cited by 36 publications

(10 citation statements)

References 55 publications

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“…ND199 ( Romero Soto et al, 2021 ), and xylitol and ethanol by yeast strains ( Shankar et al, 2020 ). Lopez-Hidalgo et al reported that the engineered strain increased 30% the coproduction of ethanol and hydrogen used wheat straw and corn stover as feedstock ( Lopez-Hidalgo et al, 2021 ). And 11.0 g/L polyhydroxybutyrate and 1.5 g/L violacein pigment were successfully co-synthesized in Iodobacter sp.…”

Section: Resultsmentioning

confidence: 99%

Coproduction of 5-Aminovalerate and δ-Valerolactam for the Synthesis of Nylon 5 From L-Lysine in Escherichia coli

Cheng

Luo

et al. 2021

Front. Bioeng. Biotechnol.

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The compounds 5-aminovalerate and δ-valerolactam are important building blocks that can be used to synthesize bioplastics. The production of 5-aminovalerate and δ-valerolactam in microorganisms provides an ideal source that reduces the cost. To achieve efficient biobased coproduction of 5-aminovalerate and δ-valerolactam in Escherichia coli, a single biotransformation step from L-lysine was constructed. First, an equilibrium mixture was formed by L-lysine α-oxidase RaiP from Scomber japonicus. In addition, by adjusting the pH and H2O2 concentration, the titers of 5-aminovalerate and δ-valerolactam reached 10.24 and 1.82 g/L from 40 g/L L-lysine HCl at pH 5.0 and 10 mM H2O2, respectively. With the optimized pH value, the δ-valerolactam titer was improved to 6.88 g/L at pH 9.0 with a molar yield of 0.35 mol/mol lysine. The ratio of 5AVA and δ-valerolactam was obviously affected by pH value. The ratio of 5AVA and δ-valerolactam could be obtained in the range of 5.63:1–0.58:1 at pH 5.0–9.0 from the equilibrium mixture. As a result, the simultaneous synthesis of 5-aminovalerate and δ-valerolactam from L-lysine in Escherichia coli is highly promising. To our knowledge, this result constitutes the highest δ-valerolactam titer reported by biological methods. In summary, a commercially implied bioprocess developed for the coproduction of 5-aminovalerate and δ-valerolactam using engineered Escherichia coli.

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Section: Resultsmentioning

confidence: 99%

Coproduction of 5-Aminovalerate and δ-Valerolactam for the Synthesis of Nylon 5 From L-Lysine in Escherichia coli

Cheng

Luo

et al. 2021

Front. Bioeng. Biotechnol.

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“…Although the success of hydrothermal pretreatment does not exclusively involve the release of xylan, XOOs has a potential market niche, and a good correlation with enzymatic saccharification has also been observed [ 30 , 31 ]. Furthermore, to have HP operating at rigorous isothermal conditions is fundamental to reach hydrogen and ethanol co-production via dark fermentation [ 32 ] and other eco-friendly fuel precursors [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Polysaccharides Release in a Laboratory-Scale Batch Hydrothermal Pretreatment of Wheat Straw under Rigorous Isothermal Operation

et al. 2021

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Hydrothermal pretreatment (HP) is an eco-friendly process for deconstructing lignocellulosic biomass (LCB) that plays a key role in ensuring the profitability of producing biofuels or bioproducts in a biorefinery. At the laboratory scale, HP is usually carried out under non-isothermal regimes with poor temperature control. In contrast, HP is usually carried out under isothermal conditions at the commercial scale. Consequently, significant discrepancies in the values of polysaccharide releases are found in the literature. Therefore, laboratory-scale HP data are not trustworthy if scale-up or retrofitting of HP at larger scales is required. This contribution presents the results of laboratory-scale batch HP for wheat straw in terms of xylan and glucan release that were obtained with rigorous temperature control under isothermal conditions during the reaction stage. The heating and cooling stages were carried out with fast rates (43 and −40 °C/min, respectively), minimizing non-isothermal reaction periods. Therefore, the polysaccharide release results can be associated exclusively with the isothermic reaction stage and can be considered as a reliable source of information for HP at commercial scales. The highest amount of xylan release was 4.8 g/L or 43% obtained at 180 °C and 20 min, while the glucan release exhibited a maximum of 1.2 g/L or 5.5%. at 160 °C/180 °C and 30 min.

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“…Biorefineries would perform a cost-effective alternative at which bio-based compounds are sided commodities concerning liquid combustible [63,64]. Imminent biorefineries would be suitable to imitate the energy performance of new oil purifying by massive heat mixture and side creation improvement.…”

Section: Structure and Applications Of Biorefineriesmentioning

confidence: 99%

Recent advances in bio-electrochemical system analysis in biorefineries

Siwal

Zhang

Saini

et al. 2021

Journal of Environmental Chemical Engineering

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Co-production of ethanol-hydrogen by genetically engineered Escherichia coli in sustainable biorefineries for lignocellulosic ethanol production

Cited by 36 publications

References 55 publications

Coproduction of 5-Aminovalerate and δ-Valerolactam for the Synthesis of Nylon 5 From L-Lysine in Escherichia coli

Coproduction of 5-Aminovalerate and δ-Valerolactam for the Synthesis of Nylon 5 From L-Lysine in Escherichia coli

Polysaccharides Release in a Laboratory-Scale Batch Hydrothermal Pretreatment of Wheat Straw under Rigorous Isothermal Operation

Recent advances in bio-electrochemical system analysis in biorefineries

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